脱落酸通过提高抗氧化酶活性与基因表达参与富氢水增强番茄幼苗抗旱性

doi:10.3969/j.issn.1004-1524.2022.09.09

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (9): 1901-1910.DOI: 10.3969/j.issn.1004-1524.2022.09.09

脱落酸通过提高抗氧化酶活性与基因表达参与富氢水增强番茄幼苗抗旱性

闫梅(), 姚彦东, 牟开萍, 淡媛媛, 李伟泰, 廖伟彪()

甘肃农业大学园艺学院,甘肃兰州 730070

收稿日期:2021-03-24 出版日期:2022-09-25 发布日期:2022-09-30
通讯作者: 廖伟彪
作者简介:*廖伟彪,E-mail: liaowb@gsau.edu.cn
闫梅(1996—),女,甘肃陇南人,硕士研究生,研究方向为园艺植物抗逆性。E-mail: 493099042@qq.com
基金资助:
国家重点研发计划(2018YFD1000800);国家自然科学基金(32072559);国家自然科学基金(31860568);国家自然科学基金(31560563);国家自然科学基金(31160398);中国博士后科学基金(20100470887);中国博士后科学基金(2012T50828);甘肃省自然科学基金(1606RJZA073);甘肃省自然科学基金(1606RJZA077);甘肃省教育厅创新创业团队项目(2018C-14)

Involvement of abscisic acid in hydrogen gas-enhanced drought resistance by improving antioxidant enzyme activity and gene expression in tomato seedlings

YAN Mei(), YAO Yandong, MOU Kaiping, DAN Yuanyuan, LI Weitai, LIAO Weibiao()

College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China

Received:2021-03-24 Online:2022-09-25 Published:2022-09-30
Contact: LIAO Weibiao

摘要/Abstract

摘要：

以15%聚乙二醇6000(PEG-6000)处理模拟干旱胁迫,研究了氢气(H₂)和脱落酸(ABA)在番茄(Solanum lycopersicum L.)幼苗抵抗干旱胁迫中的作用及其相互关系。结果表明,外源H₂和ABA均可促进番茄在干旱胁迫下的生长,其中富氢水(HRW,H₂供体)和ABA最适浓度分别为75%和150 μmol·L^-1。干旱胁迫下,HRW处理能显著增加番茄幼苗叶片内源ABA含量及其关键基因表达。同时,HRW和ABA单独及其共同处理的番茄幼苗叶片丙二醛(MDA)含量较PEG处理分别降低了34.8%、32.6%和37.0%;而超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)的活性及其基因表达显著高于PEG处理。另外,ABA抑制剂氟啶酮(FLU)抑制了HRW对干旱胁迫下番茄幼苗生长的促进作用,说明H₂促进干旱胁迫下番茄幼苗的生长可能需要ABA的参与。FLU抑制了HRW对干旱胁迫下叶片MDA含量的降低作用。此外,FLU抑制了HRW对干旱胁迫下叶片SOD、CAT和APX的活性及其基因表达的增加作用,说明H₂增强番茄幼苗抗氧化酶活性及其基因表达需要ABA的参与。可见,ABA参与了H₂增强番茄幼苗的抗旱性,这一过程是通过抑制MDA积累和提高抗氧化酶活性及其基因表达来实现的。

关键词: 干旱, 富氢水, 番茄, 丙二醛含量, 抗氧化酶活性, 基因表达

Abstract:

The 15% macrogol 6000 (PEG-6000) was used to investigate the effect of hydrogen gas (H₂) and abscisic acid (ABA) on drought resistance of tomato (Solanum lycopersicum L.) seedlings and their interaction. The results showed that both H₂ and ABA promoted the growth of tomato seedlings under drought stress, and the optimal concentrations of HRW and ABA were 75% and 150 μmol·L^-1, respectively. HRW treatment could significantly increase endogenous ABA content and the related gene expression in tomato seedling leaves under drought stress. Meanwhile, HRW and ABA alone or together significantly decreased malondialdehyde (MDA) content by 34.8%, 32.6% and 37.0%, whereas HRW and ABA significantly increased the superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activity and their gene expression in comparison with PEG treatment. Besides, fluridone (FLU), a synthetic inhibitor of ABA, significantly diminished the positive effect of HRW on tomato seedlings growth, showing that ABA was required for H₂-promoted growth of tomato seedlings under drought stress. FLU suppressed the roles of HRW in MDA content of tomato leaves under drought stress. In addition, FLU suppressed the promotive roles of HRW in the activity of SOD, CAT and APX and their gene expression, indicating that ABA was involved in H₂-enhanced the antioxidant enzyme activities and the realted gene expression under drought stress. Together,the resultsed showed that ABA might be involved in H₂-enhanced the drought resistance of tomato seedlings by decreasing the content of MDA and increasing antioxidant enzyme activities and their gene expression.

Key words: drought stress, hydrogen-rich water, tomato, malondialdehyde content, antioxidant enzyme activity, gene expression

中图分类号:

S641.2

闫梅, 姚彦东, 牟开萍, 淡媛媛, 李伟泰, 廖伟彪. 脱落酸通过提高抗氧化酶活性与基因表达参与富氢水增强番茄幼苗抗旱性[J]. 浙江农业学报, 2022, 34(9): 1901-1910.

YAN Mei, YAO Yandong, MOU Kaiping, DAN Yuanyuan, LI Weitai, LIAO Weibiao. Involvement of abscisic acid in hydrogen gas-enhanced drought resistance by improving antioxidant enzyme activity and gene expression in tomato seedlings[J]. Acta Agriculturae Zhejiangensis, 2022, 34(9): 1901-1910.

图/表 8

表1 实时荧光定量引物序列

Table 1 Primer sequences for quantitative real-time PCR

基因名称 Gene name	序列号 Accession number	正向引物 Forward primer (5'→3')	反向引物 Reverse primer (5'→3')
SlNCED SlSOD	NM-001247526 M37151.1	ACTTGAACACCCTTTGCCGA CCGATGGCGTGGCTGAAGTG	GCCGGTGGGTGTTACCTTTA CTCAGTTGTTGGACGAGCACTTGT
SlCAT	NM_001247898	GCT CCCAGTTAATGCTCCCAAGTG	GTTTGATCCTTGTCGTCCTGCTGA
SlAPX	NM_001247702	GGGAGATCCA GACCAGAGCGTAG	CTGGAGGACAATCATGGACTGTGC
Actin	SGN-U580609	AATGAACTTCGTGTGGCTCCAGAG	ATGGCAGGGGTGTTGAAGGTTTC

图1 富氢水对干旱胁迫下番茄幼苗生物量的影响同一指标不同处理间没有相同字母表示差异显著(P<0.05)。下同。

Fig.1 Effect of hydrogen-rich water on tomato seedlings biomass under drought stress The bars of the same index with different letters showed the significant difference (P<0.05). The same as below.

图2 脱落酸对干旱胁迫下番茄幼苗生物量的影响

Fig.2 Effect of ABA on tomato seedlings biomass under drought stress

图3 不同处理对干旱胁迫下番茄幼苗生物量的影响

Fig.3 Effect of various treatments on tomato seedlings biomass under drought stress

图4 HRW处理对干旱胁迫下番茄幼苗ABA含量及关键酶基因表达的影响

Fig.4 Effect of HRW treatments on ABA content and gene expression of key enzymes in tomato seedlings under drought stress

图5 不同处理对干旱胁迫下番茄幼苗丙二醛含量的影响

Fig.5 Effect of various treatments on malondialdehyde content of tomato seedlings under drought stress

图6 不同处理对干旱胁迫下番茄幼苗抗氧化酶活性的影响

Fig.6 Effect of various treatments on antioxidant enzyme activity of tomato seedlings under drought stress

图7 不同处理对干旱胁迫下番茄幼苗抗氧化酶基因表达的影响

Fig.7 Effect of various treatments on the expression of antioxidant enzyme genes of tomato seedlings under drought stress

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脱落酸通过提高抗氧化酶活性与基因表达参与富氢水增强番茄幼苗抗旱性

Involvement of abscisic acid in hydrogen gas-enhanced drought resistance by improving antioxidant enzyme activity and gene expression in tomato seedlings

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