铁皮石斛叶多糖结构及其益生性质初探

doi:10.3969/j.issn.1004-1524.2022.11.19

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (11): 2504-2511.DOI: 10.3969/j.issn.1004-1524.2022.11.19

铁皮石斛叶多糖结构及其益生性质初探

林雨晴¹^,²(), 陆胜民², 周万怡², 邢建荣², 杨颖²^,^*()

1.南京农业大学食品科技学院,江苏南京 210095
2.浙江省农业科学院食品科学研究所,浙江省果蔬保鲜与加工技术研究重点实验室,浙江杭州 310021

收稿日期:2022-03-28 出版日期:2022-11-25 发布日期:2022-11-29
作者简介:*杨颖,E-mail: ying_yang4535@sina.com
林雨晴(1997—),女,湖南邵阳人,硕士,研究方向为多糖益生特性。E-mail: 215930763@qq.com
通讯作者: 杨颖
基金资助:
浙江省重点研发计划(2020C02034)

Preliminary investigation about structure and probiotic properties of polysaccharides from Dendrobium officinale leaves

LIN Yuqing¹^,²(), LU Shengmin², ZHOU Wanyi², XING Jianrong², YANG Ying²^,^*()

1. College of Food Science and Technonlogy, Nanjing Agricultural University, Nanjing 210095, China
2. Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2022-03-28 Online:2022-11-25 Published:2022-11-29
Contact: YANG Ying

摘要/Abstract

摘要：

初步分析雁荡山地区3种石斛叶多糖的结构与益生特性,为其高值利用提供依据。以雁斛1号、雁斛3号和圣兰8号铁皮石斛的叶片为原料,提取纯化获得多糖,依次命名为DOP1、DOP2和DOP3,其平均分子量分别为2.56×10⁵、1.35×10⁵、3.26×10⁵,均由阿拉伯糖、半乳糖、葡萄糖和甘露糖组成,但其物质的量比不同。DOP1在水溶液中的组织结构最为紧密,DOP3次之,DOP2最为疏松。三者均能促进植物乳杆菌、戊糖乳杆菌和鼠李糖乳杆菌的增殖,DOP3效果最佳;DOP2和DOP3显著抑制大肠埃希菌和单增李斯特菌生长,DOP1仅对单增李斯特菌有抑制作用。三者均能提高人体结肠菌群中副拟杆菌和考拉杆菌属相对丰度,降低克雷伯氏菌属、嗜胆菌属等条件致病菌相对丰度,DOP3还可显著抑制摩根氏菌属和假单胞菌属增长。三者均可促进结肠菌群产生乙酸、丙酸及丁酸,其中,DOP2和DOP3效果优于DOP1。3种多糖均有一定的体外益生功能,DOP3的综合效果较好,具有作为新型益生因子应用的潜力。

关键词: 石斛叶, 多糖, 结肠菌群, 短链脂肪酸, 益生特性

Abstract:

To investigate the preliminary structure and probiotic properties of three Dendrobium officinale leaf polysaccharides in the Yandang Mountain area, and provide basis for high-value utilization of D. officinale leaf resources, leaves of D. officinale Yanhu No.1, D. officinale Yanhu No.3 and D. officinale Shenglan No.8 were used as raw materials, and pure polysaccharides were obtained, which were named as DOP1, DOP2 and DOP3. The relative molecular weight of DOP1, DOP2, and DOP3 were 2.56×10⁵, 1.35×10⁵ and 3.26×10⁵, respectively. All of them were composed of arabinose, galactose, glucose and mannose, but with different molar ratios. DOP1 had the tightest organizational structure in aqueous solution, followed by DOP3, and DOP2 was the loosest. The proliferation of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus rhamnosus were promoted by all three polysaccharides, among which DOP3 had the most significant effect. DOP2 and DOP3 significantly inhibited the growth of Escherichia coli and Listeria monocytogenes, while DOP1 only inhibited the growth of L. monocytogenes. The relative abundance of Parabacteroides and Koalabacteria were increased and the relative abundance of conditional pathogenic bacteria such as Klebsiella and Bliophila were decreased under the treatment of three polysaccharides in human colon flora. In addition, DOP3 also significantly inhibited the growth of Morganella and Pseudomonas. All three polysaccharides could promote the production of acetic acid, propionic acid and butyric acid, among which DOP2 and DOP3 had better effects than DOP1. The results showed all of these three polysaccharides had probiotic functions, among which DOP3 had a better comprehensive effect and had the potential to be used as a new probiotic factor.

Key words: Dendrobium officinale leaf, polysaccharide, colonic flora, short-chain fatty acid, probiotic property

中图分类号:

林雨晴, 陆胜民, 周万怡, 邢建荣, 杨颖. 铁皮石斛叶多糖结构及其益生性质初探[J]. 浙江农业学报, 2022, 34(11): 2504-2511.

LIN Yuqing, LU Shengmin, ZHOU Wanyi, XING Jianrong, YANG Ying. Preliminary investigation about structure and probiotic properties of polysaccharides from Dendrobium officinale leaves[J]. Acta Agriculturae Zhejiangensis, 2022, 34(11): 2504-2511.

图/表 8

参考文献 28

[1]	孙卓然, 刘圆, 李晓云, 等. 石斛不同种、不同药用部位中多糖含量测定[J]. 时珍国医国药, 2009, 20(8): 1886-1888.
	SUN Z R, LIU Y, LI X Y, et al. The determination of polysaccharides from several herba dendrobii and different medical parts of D. nobile Lindl, D. fimbriatum Hook, D. denneanum Kerr and D. chrysotoxum Lindl[J]. Lishizhen Medicine and Materia Medica Research, 2009, 20(8): 1886-1888. (in Chinese with English abstract)
[2]	张又元, 陈乃伟, 丁重阳, 等. 铁皮石斛茎部和叶部多糖的性质和活性[J]. 食品与生物技术学报, 2017, 36(9): 959-965.
	ZHANG Y Y, CHEN N W, DING C Y, et al. Characterization and bioactivity analysis of Dendrobium officinale stem and leaf polysacchride[J]. Journal of Food Science and Biotechnology, 2017, 36(9): 959-965. (in Chinese with English abstract)
[3]	鲍素华, 查学强, 郝杰, 等. 不同分子量铁皮石斛多糖体外抗氧化活性研究[J]. 食品科学, 2009, 30(21): 123-127.
	BAO S H, ZHA X Q, HAO J, et al. In vitro antioxidant activity of polysaccharides with different molecular weights from Dendrobium candidum[J]. Food Science, 2009, 30(21): 123-127. (in Chinese with English abstract)
[4]	LI Z, NIE K, WANG Z, et al. Quantitative structure activity relationship models for the antioxidant activity of polysaccharides[J]. PLoS One, 2016, 11(9): e0163536. DOI URL
[5]	CANFORA E E, MEEX R C R, VENEMA K, et al. Gut microbial metabolites in obesity, NAFLD and T2DM[J]. Nature Reviews Endocrinology, 2019, 15(5): 261-273. DOI PMID
[6]	RYU S T, PARK B S, BANG H T, et al. Effects of anti-heat diet and inverse lighting on growth performance, immune organ, microorganism and short chain fatty acids of broiler chickens under heat stress[J]. Journal of Environmental Biology, 2016, 37(2): 185-192.
[7]	ROOKS M G, GARRETT W S. Gut microbiota, metabolites and host immunity[J]. Nature Reviews Immunology, 2016, 16(6): 341-352. DOI PMID
[8]	邱现创, 赵宁, 李晨, 等. 铁皮石斛多糖提取工艺优化及对果蝇抗氧化能力的影响[J]. 食品科学, 2018, 39(2): 273-280.
	QIU X C, ZHAO N, LI C, et al. Optimization of extraction of polysaccharide from Dendrobium officinale and its antioxidant effect on Drosophila melanogaster[J]. Food Science, 2018, 39(2): 273-280. (in Chinese with English abstract)
[9]	焦宇知, 汪艳芝, 朱云, 等. 茶籽粕多糖的提纯及单糖组成分析[J]. 食品研究与开发, 2016, 37(22): 51-55.
	JIAO Y Z, WANG Y Z, ZHU Y, et al. Studies on purification of polysaccharides from oil-tea-cake and monosaccharides’ composition[J]. Food Research and Development, 2016, 37(22): 51-55. (in Chinese with English abstract)
[10]	刘卫宝. 黄芪多糖、寡糖的制备及其发酵益生作用的研究[D]. 无锡: 江南大学, 2020.
	LIU W B. Study on preparation of Astragalus polysaccharides and oligosaccharides and their probiotic effects[D]. Wuxi: Jiangnan University, 2020. (in Chinese with English abstract)
[11]	WICHIENCHOT S, JATUPORNPIPAT M, RASTALL R A. Oligosaccharides of pitaya (dragon fruit) flesh and their prebiotic properties[J]. Food Chemistry, 2010, 120(3): 850-857. DOI URL
[12]	ZHENG Y, XIANG S, ZHANG H, et al. Vitamin B12 enriched in spinach and its effects on gut microbiota[J]. Journal of Agricultural and Food Chemistry, 2021, 69(7): 2204-2212. DOI PMID
[13]	陈军奎, 刘伟, 王欣, 等. 成年人不同阶段肠道菌群及其代谢差异的研究[J]. 胃肠病学和肝病学杂志, 2019, 28(3): 276-281.
	CHEN J K, LIU W, WANG X, et al. Study on intestinal flora and metabolic differences in adults at different stages[J]. Chinese Journal of Gastroenterology and Hepatology, 2019, 28(3): 276-281. (in Chinese with English abstract)
[14]	唐靖雯, 卢礼平, 王欢, 等. 铁皮石斛叶的研究进展[J]. 中国民族民间医药, 2020, 29(7): 63-67.
	TANG J W, LU L P, WANG H, et al. Research progress in leaves of Dendrobium officinale Kimura et Migo[J]. Chinese Journal of Ethnomedicine and Ethnopharmacy, 2020, 29(7): 63-67. (in Chinese with English abstract)
[15]	王轶帆, 邓媛元, 张雁, 等. 龙眼多糖与燕麦多糖的结构特征及其益生活性比较[J]. 中国食品学报, 2020, 20(12): 62-71.
	WANG Y F, DENG Y Y, ZHANG Y, et al. Comparison of structure characteristics and probiotic activity of Longan polysaccharides and oat polysaccharides[J]. Journal of Chinese Institute of Food Science and Technology, 2020, 20(12): 62-71. (in Chinese with English abstract)
[16]	CLARKE S F, MURPHY E F, O'SULLIVAN O, et al. Exercise and associated dietary extremes impact on gut microbial diversity[J]. Gut, 2014, 63(12): 1913-1920. DOI PMID
[17]	欧阳建, 李秀平, 周方, 等. ‘平阳黄汤’对高脂饮食大鼠肠道屏障和肠道菌群的影响[J]. 食品科学, 2021, 42(23): 170-181.
	OUYANG J, LI X P, ZHOU F, et al. Effect of Pingyang yellow tea on intestinal barrier and intestinal flora in rats fed high-fat diet[J]. Food Science, 2021, 42(23): 170-181. (in Chinese with English abstract)
[18]	WANG K, LIAO M F, ZHOU N, et al. Parabacteroides distasonis alleviates obesity and metabolic dysfunctions via production of succinate and secondary bile acids[J]. Cell Reports, 2019, 26(1): 222-235.e5. DOI URL
[19]	MUÑIZ PEDROGO D A, JENSEN M D, VAN DYKE C T, et al. Gut microbial carbohydrate metabolism hinders weight loss in overweight adults undergoing lifestyle intervention with a volumetric diet[J]. Mayo Clinic Proceedings, 2018, 93(8): 1104-1110. DOI PMID
[20]	MALDONADO-ARRIAGA B, SANDOVAL-JIMÉNEZ S, ROD-RÍGUEZ-SILVERIO J, et al. Gut dysbiosis and clinical phases of pancolitis in patients with ulcerative colitis[J]. MicrobiologyOpen, 2021, 10(2): e1181.
[21]	武爱荣, 杨乐. VITEK-2 Compact检测奇异变形杆菌、摩根摩根菌、铜绿假单胞菌的部分药敏结果准确性评价[J]. 现代检验医学杂志, 2020, 35(6): 106-110.
	WU A R, YANG L. Accuracy evaluation of partial drug sensitivity results of VITEK-2 Compact detection for Proteus singularis, morgan morgan and Pseudomonas aeruginosa[J]. Journal of Modern Laboratory Medicine, 2020, 35(6): 106-110. (in Chinese with English abstract)
[22]	MORTENSEN P B, CLAUSEN M R. Short-chain fatty acids in the human colon: relation to gastrointestinal health and disease[J]. Scandinavian Journal of Gastroenterology, 1996, 31(Suppl 216): 132-148.
[23]	TURNBAUGH P J, LEY R E, MAHOWALD M A, et al. An obesity-associated gut microbiome with increased capacity for energy harvest[J]. Nature, 2006, 444(7122): 1027-1031. DOI URL
[24]	AGUILERA A, SELGAS R, DIÉZ J J, et al. Anorexia in end-stage renal disease: pathophysiology and treatment[J]. Expert Opinion on Pharmacotherapy, 2001, 2(11): 1825-1838. PMID
[25]	WU D T, NIE X R, GAN R Y, et al. In vitro digestion and fecal fermentation behaviors of a pectic polysaccharide from okra (Abelmoschus esculentus) and its impacts on human gut microbiota[J]. Food Hydrocolloids, 2021, 114: 106577. DOI URL
[26]	DO PRADO S B R, MINGUZZI B T, HOFFMANN C, et al. Modulation of human gut microbiota by dietary fibers from unripe and ripe papayas: distinct polysaccharide degradation using a colonic in vitro fermentation model[J]. Food Chemistry, 2021, 348: 129071. DOI URL
[27]	CANFORA E E, JOCKEN J W, BLAAK E E. Short-chain fatty acids in control of body weight and insulin sensitivity[J]. Nature Reviews Endocrinology, 2015, 11(10): 577-591. DOI PMID
[28]	WU J, LIU Y, DOU Z, et al. Black garlic melanoidins prevent obesity, reduce serum LPS levels and modulate the gut microbiota composition in high-fat diet-induced obese C57BL/6J mice[J]. Food & Function, 2020, 11(11): 9585-9598.

名称 Name	保留时间 Retention time/min	数均分子量 Number-average molecular weight	重均分子量 Weight-average molecular weight	峰位分子量 Peak molecular weight
DOP1	15.22	7.16×10⁴	2.56×10⁵	1.77×10⁵
DOP2	16.44	4.14×10⁴	1.35×10⁵	4.56×10⁴
DOP3	15.61	8.74×10⁴	3.26×10⁵	1.14×10⁵

名称 Name	保留时间 Retention time/min	数均分子量 Number-average molecular weight	重均分子量 Weight-average molecular weight	峰位分子量 Peak molecular weight
DOP1	15.22	7.16×10⁴	2.56×10⁵	1.77×10⁵
DOP2	16.44	4.14×10⁴	1.35×10⁵	4.56×10⁴
DOP3	15.61	8.74×10⁴	3.26×10⁵	1.14×10⁵

名称 Name	葡萄糖 Glucose	甘露糖 Mannose	半乳糖 Galactose	阿拉伯糖 Arabinose
DOP1	252	210	1	0.78
DOP2	370	245	1	0.87
DOP3	278	169	1	0.90

名称 Name	葡萄糖 Glucose	甘露糖 Mannose	半乳糖 Galactose	阿拉伯糖 Arabinose
DOP1	252	210	1	0.78
DOP2	370	245	1	0.87
DOP3	278	169	1	0.90

碳源 Carbon source	菌落总数 Total viable count
碳源 Carbon source	植物乳杆菌 Lactobacillus plantarum	戊糖乳杆菌 Lactobacillus pentosus	干酪乳杆菌 Lactobacillus casei	鼠李糖乳杆菌 Lactobacillus rhamnosus
Glu(CK)	1.8±0.6 b	12.9±1.2 b	6.4±0.4 b	1.3±0.1 de
DOP1	3.2±0.5 b	7.0±1.2 c	2.3±0.5 d	1.2±0.1 de
DOP2	3.3±0.2 b	4.6±0.8 d	3.5±0.4 d	2.9±0.8 bc
DOP3	2.8.±6.0 b	6.1±1.4 cd	2.7±0.3 d	1.1±0.1 e
Glu+DOP1	2.7±0.6 b	13.0±1.4 b	9.1±1.2 a	2.9±0.5 ab
Glu+DOP2	3.9±0.3 b	15.8±4.7 b	7.4±1.1 b	3.2±0.7 a
Glu+DOP3	14.9±0.4 a	18.7±0.9 a	6.7±0.5 b	2.0±0.1 cd

铁皮石斛叶多糖结构及其益生性质初探

Preliminary investigation about structure and probiotic properties of polysaccharides from Dendrobium officinale leaves

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 28

相关文章 15

编辑推荐

Metrics

本文评价

碳源 Carbon source	菌落总数 Total viable count
碳源 Carbon source	大肠埃希菌 Escherichia coli	金黄色葡萄球菌 Staphylococcus aureus	单增李斯特菌 Listeria monocytogenes
Glu(CK)	6.5±0.7 a	2.8±0.1 a	10.0±0.9 a
DOP1	5.4±0.6 a	3.0±0.1 a	8.0±0.4 b
DOP2	3.2±0.6 b	3.5±0.5 a	7.0±0.1 b
DOP3	1.6±0.2 c	3.8±0.8 a	6.6±0.8 b

[1]	陈琳, 顾青. 柳叶蜡梅多糖对大鼠急性胃黏膜损伤的保护机制研究[J]. 浙江农业学报, 2025, 37(5): 1009-1016.
[2]	吴兴凤, 肖英平, 吕文涛, 马灵燕, 陈渠, 温洋, 徐娥. 丁酸梭菌通过调节结肠微生物结构和短链脂肪酸含量影响小鼠肌纤维类型[J]. 浙江农业学报, 2025, 37(3): 568-578.
[3]	乔慧茹, 房祥军, 吴伟杰, 刘瑞玲, 陈杭君, 邓尚贵, 沙浩, 郜海燕. 蓝莓与铁皮石斛叶复合乳酸菌发酵饮料工艺优化与品质分析[J]. 浙江农业学报, 2025, 37(3): 654-666.
[4]	黄浩, 汤涛, 许振岚, 赵学平. 吡唑醚菌酯对铁皮石斛中多糖和黄酮的影响研究[J]. 浙江农业学报, 2025, 37(1): 115-125.
[5]	张妮, 陶文扬, 罗梦帆, 周万怡, 郑晓杰, 李彦坡, 金火喜, 杨颖. 酶解辅助提取对铁皮石斛多糖结构和菌群调节功能的影响[J]. 浙江农业学报, 2024, 36(9): 2099-2109.
[6]	赵小亮, 鲁雲, 康兴兴, 龙则宇, 郑晓杰. 雁荡山铁皮石斛多糖的提取、结构表征与体外抗氧化活性[J]. 浙江农业学报, 2024, 36(8): 1898-1908.
[7]	曹乃馨, 罗阳兰, 阎勇, 解修超, 张雯龙. 桑树桑黄JM-1胞外多糖液态培养基优化及其抗氧化性研究[J]. 浙江农业学报, 2024, 36(6): 1245-1255.
[8]	温婉宁, 王晨, 胡连花, 房志家, 邓旗, 孙力军. 海蛾水提取物对小鼠系统低度炎症的干预作用[J]. 浙江农业学报, 2024, 36(12): 2784-2793.
[9]	吕国英, 王梦雨, 张作法. 猴头菇多糖对小鼠肠道菌群的影响[J]. 浙江农业学报, 2024, 36(12): 2794-2802.
[10]	李镜锐, 陶文扬, 杨颖, 周万怡, 陆胜民, 王阳光. 三种浙产铁皮石斛多糖的结构及免疫功效探究[J]. 浙江农业学报, 2023, 35(8): 1888-1895.
[11]	张璐, 李翘楚, 王增利, 丁强, 王鸿磊. 金耳类酵母型菌株分离与高产胞外多糖培养基优化[J]. 浙江农业学报, 2023, 35(5): 1154-1160.
[12]	夏伦斌, 马龙龙, 乔德亮, 何燕飞, 蒋平. 三角帆蚌多糖对肉仔鸡生长性能、抗氧化及免疫功能的影响[J]. 浙江农业学报, 2023, 35(3): 547-555.
[13]	马波, 陶震, 周瑞, 王雪, 吕茜茜, 孙士红, 王寒, 高金秋, 张楚涵, 陈凤清. 花叶万年青功能成分提取条件优化与活性探究[J]. 浙江农业学报, 2023, 35(2): 383-393.
[14]	陆玲鸿, 马媛媛, 古咸彬, 肖金平, 宋根华, 张慧琴. 猕猴桃果实软化过程中细胞壁多糖物质含量与果胶降解相关酶活性变化[J]. 浙江农业学报, 2022, 34(12): 2648-2658.
[15]	吴卫成, 戴建波, 曹艳, 夏其乐, 陈剑兵, 孟祥河. 物理改性对甘薯皮膳食纤维含量、多糖组成及其结构的影响[J]. 浙江农业学报, 2020, 32(3): 490-498.