铁皮石斛叶多糖结构及其益生性质初探

doi:10.3969/j.issn.1004-1524.2022.11.19

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (11): 2504-2511.DOI: 10.3969/j.issn.1004-1524.2022.11.19

铁皮石斛叶多糖结构及其益生性质初探

林雨晴¹^,²(), 陆胜民², 周万怡², 邢建荣², 杨颖²^,^*()

1.南京农业大学食品科技学院,江苏南京 210095
2.浙江省农业科学院食品科学研究所,浙江省果蔬保鲜与加工技术研究重点实验室,浙江杭州 310021

收稿日期:2022-03-28 出版日期:2022-11-25 发布日期:2022-11-29
通讯作者: 杨颖
作者简介:*杨颖,E-mail: ying_yang4535@sina.com
林雨晴(1997—),女,湖南邵阳人,硕士,研究方向为多糖益生特性。E-mail: 215930763@qq.com
基金资助:
浙江省重点研发计划(2020C02034)

Preliminary investigation about structure and probiotic properties of polysaccharides from Dendrobium officinale leaves

LIN Yuqing¹^,²(), LU Shengmin², ZHOU Wanyi², XING Jianrong², YANG Ying²^,^*()

1. College of Food Science and Technonlogy, Nanjing Agricultural University, Nanjing 210095, China
2. Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2022-03-28 Online:2022-11-25 Published:2022-11-29
Contact: YANG Ying

摘要/Abstract

摘要：

初步分析雁荡山地区3种石斛叶多糖的结构与益生特性,为其高值利用提供依据。以雁斛1号、雁斛3号和圣兰8号铁皮石斛的叶片为原料,提取纯化获得多糖,依次命名为DOP1、DOP2和DOP3,其平均分子量分别为2.56×10⁵、1.35×10⁵、3.26×10⁵,均由阿拉伯糖、半乳糖、葡萄糖和甘露糖组成,但其物质的量比不同。DOP1在水溶液中的组织结构最为紧密,DOP3次之,DOP2最为疏松。三者均能促进植物乳杆菌、戊糖乳杆菌和鼠李糖乳杆菌的增殖,DOP3效果最佳;DOP2和DOP3显著抑制大肠埃希菌和单增李斯特菌生长,DOP1仅对单增李斯特菌有抑制作用。三者均能提高人体结肠菌群中副拟杆菌和考拉杆菌属相对丰度,降低克雷伯氏菌属、嗜胆菌属等条件致病菌相对丰度,DOP3还可显著抑制摩根氏菌属和假单胞菌属增长。三者均可促进结肠菌群产生乙酸、丙酸及丁酸,其中,DOP2和DOP3效果优于DOP1。3种多糖均有一定的体外益生功能,DOP3的综合效果较好,具有作为新型益生因子应用的潜力。

关键词: 石斛叶, 多糖, 结肠菌群, 短链脂肪酸, 益生特性

Abstract:

To investigate the preliminary structure and probiotic properties of three Dendrobium officinale leaf polysaccharides in the Yandang Mountain area, and provide basis for high-value utilization of D. officinale leaf resources, leaves of D. officinale Yanhu No.1, D. officinale Yanhu No.3 and D. officinale Shenglan No.8 were used as raw materials, and pure polysaccharides were obtained, which were named as DOP1, DOP2 and DOP3. The relative molecular weight of DOP1, DOP2, and DOP3 were 2.56×10⁵, 1.35×10⁵ and 3.26×10⁵, respectively. All of them were composed of arabinose, galactose, glucose and mannose, but with different molar ratios. DOP1 had the tightest organizational structure in aqueous solution, followed by DOP3, and DOP2 was the loosest. The proliferation of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus rhamnosus were promoted by all three polysaccharides, among which DOP3 had the most significant effect. DOP2 and DOP3 significantly inhibited the growth of Escherichia coli and Listeria monocytogenes, while DOP1 only inhibited the growth of L. monocytogenes. The relative abundance of Parabacteroides and Koalabacteria were increased and the relative abundance of conditional pathogenic bacteria such as Klebsiella and Bliophila were decreased under the treatment of three polysaccharides in human colon flora. In addition, DOP3 also significantly inhibited the growth of Morganella and Pseudomonas. All three polysaccharides could promote the production of acetic acid, propionic acid and butyric acid, among which DOP2 and DOP3 had better effects than DOP1. The results showed all of these three polysaccharides had probiotic functions, among which DOP3 had a better comprehensive effect and had the potential to be used as a new probiotic factor.

Key words: Dendrobium officinale leaf, polysaccharide, colonic flora, short-chain fatty acid, probiotic property

中图分类号:

林雨晴, 陆胜民, 周万怡, 邢建荣, 杨颖. 铁皮石斛叶多糖结构及其益生性质初探[J]. 浙江农业学报, 2022, 34(11): 2504-2511.

LIN Yuqing, LU Shengmin, ZHOU Wanyi, XING Jianrong, YANG Ying. Preliminary investigation about structure and probiotic properties of polysaccharides from Dendrobium officinale leaves[J]. Acta Agriculturae Zhejiangensis, 2022, 34(11): 2504-2511.

图/表 8

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名称 Name	保留时间 Retention time/min	数均分子量 Number-average molecular weight	重均分子量 Weight-average molecular weight	峰位分子量 Peak molecular weight
DOP1	15.22	7.16×10⁴	2.56×10⁵	1.77×10⁵
DOP2	16.44	4.14×10⁴	1.35×10⁵	4.56×10⁴
DOP3	15.61	8.74×10⁴	3.26×10⁵	1.14×10⁵

名称 Name	保留时间 Retention time/min	数均分子量 Number-average molecular weight	重均分子量 Weight-average molecular weight	峰位分子量 Peak molecular weight
DOP1	15.22	7.16×10⁴	2.56×10⁵	1.77×10⁵
DOP2	16.44	4.14×10⁴	1.35×10⁵	4.56×10⁴
DOP3	15.61	8.74×10⁴	3.26×10⁵	1.14×10⁵

名称 Name	葡萄糖 Glucose	甘露糖 Mannose	半乳糖 Galactose	阿拉伯糖 Arabinose
DOP1	252	210	1	0.78
DOP2	370	245	1	0.87
DOP3	278	169	1	0.90

名称 Name	葡萄糖 Glucose	甘露糖 Mannose	半乳糖 Galactose	阿拉伯糖 Arabinose
DOP1	252	210	1	0.78
DOP2	370	245	1	0.87
DOP3	278	169	1	0.90

碳源 Carbon source	菌落总数 Total viable count
碳源 Carbon source	植物乳杆菌 Lactobacillus plantarum	戊糖乳杆菌 Lactobacillus pentosus	干酪乳杆菌 Lactobacillus casei	鼠李糖乳杆菌 Lactobacillus rhamnosus
Glu(CK)	1.8±0.6 b	12.9±1.2 b	6.4±0.4 b	1.3±0.1 de
DOP1	3.2±0.5 b	7.0±1.2 c	2.3±0.5 d	1.2±0.1 de
DOP2	3.3±0.2 b	4.6±0.8 d	3.5±0.4 d	2.9±0.8 bc
DOP3	2.8.±6.0 b	6.1±1.4 cd	2.7±0.3 d	1.1±0.1 e
Glu+DOP1	2.7±0.6 b	13.0±1.4 b	9.1±1.2 a	2.9±0.5 ab
Glu+DOP2	3.9±0.3 b	15.8±4.7 b	7.4±1.1 b	3.2±0.7 a
Glu+DOP3	14.9±0.4 a	18.7±0.9 a	6.7±0.5 b	2.0±0.1 cd

铁皮石斛叶多糖结构及其益生性质初探

Preliminary investigation about structure and probiotic properties of polysaccharides from Dendrobium officinale leaves

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碳源 Carbon source	菌落总数 Total viable count
碳源 Carbon source	大肠埃希菌 Escherichia coli	金黄色葡萄球菌 Staphylococcus aureus	单增李斯特菌 Listeria monocytogenes
Glu(CK)	6.5±0.7 a	2.8±0.1 a	10.0±0.9 a
DOP1	5.4±0.6 a	3.0±0.1 a	8.0±0.4 b
DOP2	3.2±0.6 b	3.5±0.5 a	7.0±0.1 b
DOP3	1.6±0.2 c	3.8±0.8 a	6.6±0.8 b

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