拟南芥N末端乙酰转移酶Naa50参与调控根细胞有丝分裂

doi:10.3969/j.issn.1004-1524.2022.12.03

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (12): 2603-2609.DOI: 10.3969/j.issn.1004-1524.2022.12.03

拟南芥N末端乙酰转移酶Naa50参与调控根细胞有丝分裂

冯金林(), 席晓宇, 赵世凤

山西师范大学生命科学学院,山西太原 030031

收稿日期:2022-03-22 出版日期:2022-12-25 发布日期:2022-12-26
作者简介:冯金林(1986—),男,河北泊头人,博士,副教授,研究方向为植物发育生物学。E-mail:jinlin_feng@163.com
基金资助:
山西省回国留学人员科研资助项目(2020-091);山西省留学人员科技活动择优资助项目(20210045)

Arabidopsis N-terminal acetyltransferase Naa50 is involved in regulation of root cell mitosis

FENG Jinlin(), XI Xiaoyu, ZHAO Shifeng

College of Life Science, Shanxi Normal University, Taiyuan 030031, China

Received:2022-03-22 Online:2022-12-25 Published:2022-12-26

摘要/Abstract

摘要：

N末端乙酰转移酶Naa50在调控拟南芥生长发育方面至关重要。为探究Naa50是否通过调控细胞有丝分裂来影响植物生长发育,将naa50-1突变体与H2B-YFP株系杂交,利用H2B-YFP标示细胞分裂过程中染色体的分离行为。结果表明,与野生型相比,拟南芥naa50-1突变体根尖分生区细胞有丝分裂指数下降,染色体畸变率明显增加,微核率上升,且处在分裂中期的细胞比例增加。将naa50-1突变体与CYCB1-CUS株系杂交,检测根细胞中CYCB1蛋白表达,发现naa50-1突变体中CYCB1蛋白表达量明显增加。PI染色分析表明,naa50-1突变体根部存在部分死细胞。以上结果表明,在细胞有丝分裂过程中,Naa50促进遗传物质平均分配到两个子细胞中,促进细胞从分裂中期向后期转换;Naa50通过调控细胞分裂进而参与植物生长发育的调控。

关键词: 拟南芥, N末端乙酰转移酶Naa50, 有丝分裂指数, 细胞分裂

Abstract:

N-terminal acetyltransferase Naa50 plays an important role in regulating growth and development in Arabidopsis. In order to explore whether Naa50 affects plant growth and development by regulating cell mitosis, the naa50-1 mutant was crossed with H2B-YFP line, and the segregation behavior of chromosomes during cell division was detected by H2B-YFP. The results showed that compared with the wild-type, the cell mitotic index in root meristem region of naa50-1 mutant decreased, chromosome aberration rate and micronucleus rate increased, and so as the proportion of cells in metaphase increased. The expression of CYCB1 protein in root cells was detected by crossing of naa50-1 mutant with CYCB1-CUS, and it was found that the expression of CYCB1 protein in naa50-1 mutant was increased. PI staining analysis showed that there were several dead cells in the root of naa50-1 mutant. These results indicated that Naa50 promotes the equal distribution of genetic material between two daughter cells during mitosis and the transition from metaphase to anaphase. Overall, Naa50 is involved in the regulation of plant growth and development by regulating cell division.

Key words: Arabidopsis, N-terminal acetyltransferase Naa50, mitotic index, cell mitosis

中图分类号:

Q945.3

冯金林, 席晓宇, 赵世凤. 拟南芥N末端乙酰转移酶Naa50参与调控根细胞有丝分裂[J]. 浙江农业学报, 2022, 34(12): 2603-2609.

FENG Jinlin, XI Xiaoyu, ZHAO Shifeng. Arabidopsis N-terminal acetyltransferase Naa50 is involved in regulation of root cell mitosis[J]. Acta Agriculturae Zhejiangensis, 2022, 34(12): 2603-2609.

图/表 5

图1 野生型和naa50-1突变体根尖细胞细胞核表型观察利用H2B-YFP对萌发5 d的拟南芥根尖细胞细胞核形态的观察。标尺=20 μm。

Fig.1 Observation of nuclear phenotype in root tip cells of wild-type and naa50-1 mutant Observation of nuclear morphology of root tip cells in 5-day-old Arabidopsis by using H2B-YFP. Bar=20 μm.

图2 拟南芥根尖分生区细胞不同时期有丝分裂与不同类型染色体畸变图A、B、C、D分别为野生型细胞分裂的前期、中期、后期、末期;图E、F、G、H为naa50-1突变体细胞分裂前期、中期、后期、末期;图I为微核;图J为染色体桥;图K为多束分裂;图L为不等分裂;图M为细胞板偏转;图N为双核现象。标尺=5 μm。

Fig.2 Mitosis and chromosomal aberration of Arabidopsis root apical meristem cells at different stages A, B, C and D are showing the wild-type cells at prophase, metaphase, anaphase and telophase respectively; E, F, G and H are showing the naa50-1 mutant cells at prophase, metaphase, anaphase and telophase respectively; I, Cell with micronucleus; J, Cell with chromosome bridge; K, Cell with multiple bundles of chromosome; L, Asymmetric division cell; M, Cell with plate migration; N, Cell with two nuclei; Bar=5 μm.

表1 Naa50对拟南芥根尖分生区细胞染色体畸变率的影响

Table 1 Effects of Naa50 on chromosome aberration rate and of Arabidopsis root apical meristem cells

基因型 Genotype	TNC	NAC	NCM	NCB	NCC	NAD	CPM	CTN	CFC	PCA/%	PCM/%
野生型Wild-type	3 254	66	3	12	1	10	18	1	20	2.0	0.09
naa50-1	2 265	253	14	17	14	35	130	2	41	11.2	0.62

表2 Naa50对拟南芥根尖分生区细胞有丝分裂率的影响

Table 2 Effects of Naa50 on mitotic rate of Arabidopsis root apical meristem cells

基因型Genotype	TNC	NMS	NCP	NCM	NCA	NCT	MI/%
野生型Wild-type	3254	426	47	20(0.6%)	164	195	13.0
naa50-1	2265	241	38	34(1.5%)	114	55	10.6

图3 拟南芥根尖细胞中CYCB1的表达和PI染色 A,利用CYCB1-GUS对生长5 d的拟南芥根中CYCB1的表达量进行检测;B,对生长5 d的拟南芥根尖进行PI染色;标尺=50 μm。

Fig.3 Expression of CYCB1 and PI staining in the root cells of Arabidopsis A, Detection of the expression of CYCB1 in the roots of 5-day-old Arabidopsis by using CYCB1-GUS; B, PI staining in the root tips of 5-day-old Arabidopsis; Bar=50 μm.

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拟南芥N末端乙酰转移酶Naa50参与调控根细胞有丝分裂

Arabidopsis N-terminal acetyltransferase Naa50 is involved in regulation of root cell mitosis

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