不同贮藏条件对3种花粉源蜂王浆品质的影响

doi:10.3969/j.issn.1004-1524.2023.05.20

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (5): 1161-1167.DOI: 10.3969/j.issn.1004-1524.2023.05.20

不同贮藏条件对3种花粉源蜂王浆品质的影响

肖立涵¹^,²(), 辛美果¹^,^*(), 卢文静², 叶沁², 张岑², 肖朝耿², 谌迪²^,^*()

1.佛山科学技术学院食品科学与工程学院,广东佛山 528231
2.浙江省农业科学院食品科学研究所,浙江杭州 310021

收稿日期:2022-04-12 出版日期:2023-05-25 发布日期:2023-06-01
作者简介:肖立涵(1998—),男,广东潮汕人,硕士研究生,研究方向为食品营养与功能。E-mail: 957607991@qq.com
通讯作者: *辛美果,E-mail: 1684577665@qq.com;谌迪,E-mail: cd0sindy@163.com
基金资助:
国家自然科学基金(32201930);浙江省重点研发计划项目(2019C02069)

Effects of different storage conditions on quality of royal jelly from three pollen sources

XIAO Lihan¹^,²(), XIN Meiguo¹^,^*(), LU Wenjing², YE Qin², ZHANG Cen², XIAO Chaogeng², CHEN Di²^,^*()

1. College of Food Science and Engineering, Foshan University, Foshan 528231, Guangdong, China
2. Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2022-04-12 Online:2023-05-25 Published:2023-06-01

摘要/Abstract

摘要：

为研究贮藏温度和贮藏时间对蜂王浆品质的影响,以椴树浆、油菜浆、洋槐浆3种不同来源的蜂王浆为原料,通过测定其总蛋白、10-羟基-2-癸烯酸(10-HDA)、游离糖、游离氨基酸和游离脂肪酸含量,同时分析其羟自由基和DPPH自由基清除能力的变化规律。结果表明,不同蜜源蜂王浆之间总蛋白含量和10-HDA含量存在显著差异,短期贮藏不会导致其发生显著变化,但会导致油菜浆中葡萄糖和果糖等单糖含量的升高以及蔗糖含量的显著减少(P<0.05)。不同花粉源蜂王浆中氨基酸和游离脂肪酸组成类似,短期贮藏后油菜浆中的谷氨酸、棕榈酸、油酸、亚油酸和α-亚麻酸显著增加,椴树浆中丙氨酸、油酸及洋槐浆中亮氨酸和组氨酸则出现降低的现象。3种不同来源的蜂王浆的羟自由基和DPPH自由基清除能力在贮藏后显著降低(P<0.05)。本研究可以为蜂王浆在化学成分及抗氧化活性变化等方面的品质评价提供理论参考。

关键词: 蜂王浆, 贮藏, 温度, 品质, 抗氧化能力

Abstract:

To study the effect of storage temperature and storage time on the quality of royal jelly, the contents of total protein, 10-HDA, free sugar, free amino acid and free fatty acid of royal jelly were determined on linden tree royal jelly, cole royal jelly and robinia royal jelly. The scavenging ability of hydroxyl radical and DPPH radical were also analyzed. The results showed that there were significant differences in total protein content and 10-HDA content among different nectar sources. Short-term storage did not cause significant changes in total protein content and 10-HDA content, but the content of glucose and fructose of cole royal jelly increased and the content of sucrose decreased significantly (P<0.05). The contents of amino acids and free fatty acids were similar. After short-term storage, the contents of glutamic acid, palmitic acid, oleic acid, linoleic acid and α-linolenic acid in cole royal jelly were significantly increased, while alanine, oleic acid in linden tree royal jelly and leucine and histidine in robinia royal jelly were decreased. The scavenging abilities of hydroxyl radical and DPPH free radical in three kinds of royal jelly from different sources were significantly decreased after storage (P<0.05). This study can provide theoretical reference for the quality evaluation of royal jelly in chemical composition and antioxidant activity.

Key words: royal jelly, storage, temperature, quality, antioxidant activity

中图分类号:

S896.3

肖立涵, 辛美果, 卢文静, 叶沁, 张岑, 肖朝耿, 谌迪. 不同贮藏条件对3种花粉源蜂王浆品质的影响[J]. 浙江农业学报, 2023, 35(5): 1161-1167.

XIAO Lihan, XIN Meiguo, LU Wenjing, YE Qin, ZHANG Cen, XIAO Chaogeng, CHEN Di. Effects of different storage conditions on quality of royal jelly from three pollen sources[J]. Acta Agriculturae Zhejiangensis, 2023, 35(5): 1161-1167.

图/表 5

参考文献 26

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氨基酸种类 Amino acid type	椴树浆Linden tree/(g·kg^-1)		油菜浆Cole/(g·kg^-1)		洋槐浆Robinia/(g·kg^-1)
氨基酸种类 Amino acid type	贮藏前 Before storage	贮藏后 After storage	贮藏前 Before storage	贮藏后 After storage	贮藏前 Before storage	贮藏后 After storage
天门冬氨酸Aspartic acid	0.145	0.151	0.150	00.153	0.162	0.166
苏氨酸Threonine	0	0	0	0	0	0
丝氨酸Serine	0.010	0.009	0.009	0.009	0.007	0.007
谷氨酸Glutamic acid	0.428	0.439	0.094	0.462	0.431	0.437
甘氨酸Glycine	0.024	0.023	0.025	0.024	0.025	0.024
丙氨酸Alanine	0.021	0.016	0.020	0.019	0.024	0.024
缬氨酸Valine	0.023	0.022	0.026	0.029	0.036	0.037
蛋氨酸Methionine	0	0	0	0	0	0
异亮氨酸Isoleucine	0.020	0.021	0.021	0.022	0.018	0.017
亮氨酸Leucine	0.017	0.016	0.018	0.016	0.017	0.015
酪氨酸Tyrosine	0	0	0	0	0	0
苯丙氨酸Phenylalanine	0	0	0	0	0	0
组氨酸Histidine	0.100	0.091	0.102	0.092	0.125	0.117
赖氨酸Lysine	2.899	2.873	2.699	2.680	2.259	2.253
精氨酸Arginine	0.265	0.265	0.267	0.273	0.255	0.253
脯氨酸Proline	3.516	3.584	3.646	3.675	4.226	4.256

氨基酸种类 Amino acid type	椴树浆Linden tree/(g·kg^-1)		油菜浆Cole/(g·kg^-1)		洋槐浆Robinia/(g·kg^-1)
氨基酸种类 Amino acid type	贮藏前 Before storage	贮藏后 After storage	贮藏前 Before storage	贮藏后 After storage	贮藏前 Before storage	贮藏后 After storage
天门冬氨酸Aspartic acid	0.145	0.151	0.150	00.153	0.162	0.166
苏氨酸Threonine	0	0	0	0	0	0
丝氨酸Serine	0.010	0.009	0.009	0.009	0.007	0.007
谷氨酸Glutamic acid	0.428	0.439	0.094	0.462	0.431	0.437
甘氨酸Glycine	0.024	0.023	0.025	0.024	0.025	0.024
丙氨酸Alanine	0.021	0.016	0.020	0.019	0.024	0.024
缬氨酸Valine	0.023	0.022	0.026	0.029	0.036	0.037
蛋氨酸Methionine	0	0	0	0	0	0
异亮氨酸Isoleucine	0.020	0.021	0.021	0.022	0.018	0.017
亮氨酸Leucine	0.017	0.016	0.018	0.016	0.017	0.015
酪氨酸Tyrosine	0	0	0	0	0	0
苯丙氨酸Phenylalanine	0	0	0	0	0	0
组氨酸Histidine	0.100	0.091	0.102	0.092	0.125	0.117
赖氨酸Lysine	2.899	2.873	2.699	2.680	2.259	2.253
精氨酸Arginine	0.265	0.265	0.267	0.273	0.255	0.253
脯氨酸Proline	3.516	3.584	3.646	3.675	4.226	4.256

脂肪酸种类 Fatty acid type	椴树浆Linden tree/(g·kg^-1)		油菜浆Cole/(g·kg^-1)		洋槐浆Robinia/(g·kg^-1)
脂肪酸种类 Fatty acid type	贮藏前 Before storage	贮藏后 After storage	贮藏前 Before storage	贮藏后 After storage	贮藏前 Before storage	贮藏后 After storage
辛酸Caprylic acid	0.001 950	0.001 910	0.002 130	0.002 220	0.001 570	0.001 550
癸酸Capric acid	0.000 833	0.000 900	0.000 706	0.000 878	0.000 780	0.001 020
棕榈酸Palmitic acid	0.015 900	0.014 200	0.014 300	0.020 500	0.013 700	0.014 200
硬脂酸Stearic acid	—	—	—	0.007 130	—	—
油酸Oleic acid	0.011 700	0.007 870	0.006 980	0.010 300	0.008 220	0.007 990
亚油酸Linoleic acid	0.004 060	0.006 380	0.004 500	0.005 600	0.004 550	0.004 440
α-亚麻酸α-linolenic acid	0.004 120	0.004 980	0.003 050	0.004 280	0.004 290	0.004 440
二十二碳酸	0.022 500	0.024 600	0.030 600	0.036 500	0.034 400	0.030 900
Behenic acid(C22:0)

脂肪酸种类 Fatty acid type	椴树浆Linden tree/(g·kg^-1)		油菜浆Cole/(g·kg^-1)		洋槐浆Robinia/(g·kg^-1)
脂肪酸种类 Fatty acid type	贮藏前 Before storage	贮藏后 After storage	贮藏前 Before storage	贮藏后 After storage	贮藏前 Before storage	贮藏后 After storage
辛酸Caprylic acid	0.001 950	0.001 910	0.002 130	0.002 220	0.001 570	0.001 550
癸酸Capric acid	0.000 833	0.000 900	0.000 706	0.000 878	0.000 780	0.001 020
棕榈酸Palmitic acid	0.015 900	0.014 200	0.014 300	0.020 500	0.013 700	0.014 200
硬脂酸Stearic acid	—	—	—	0.007 130	—	—
油酸Oleic acid	0.011 700	0.007 870	0.006 980	0.010 300	0.008 220	0.007 990
亚油酸Linoleic acid	0.004 060	0.006 380	0.004 500	0.005 600	0.004 550	0.004 440
α-亚麻酸α-linolenic acid	0.004 120	0.004 980	0.003 050	0.004 280	0.004 290	0.004 440
二十二碳酸	0.022 500	0.024 600	0.030 600	0.036 500	0.034 400	0.030 900
Behenic acid(C22:0)

不同贮藏条件对3种花粉源蜂王浆品质的影响

Effects of different storage conditions on quality of royal jelly from three pollen sources

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