浙江农业学报 ›› 2023, Vol. 35 ›› Issue (6): 1315-1327.DOI: 10.3969/j.issn.1004-1524.2023.06.09
张新业1,2,3(), 李文静1,2,3, 朱姝1, 孙艳香1, 王聪艳1, 闫训友1, 周志国1,*(
)
收稿日期:
2022-06-06
出版日期:
2023-06-25
发布日期:
2023-07-04
通讯作者:
*周志国,E-mail: zhiguo_zhou@163.com
作者简介:
张新业(1986—),男,山东潍坊人,博士研究生,讲师,主要从事植物分子育种研究。E-mail:zhigancao@126.com
基金资助:
ZHANG Xinye1,2,3(), LI Wenjing1,2,3, ZHU Shu1, SUN Yanxiang1, WANG Congyan1, YAN Xunyou1, ZHOU Zhiguo1,*(
)
Received:
2022-06-06
Online:
2023-06-25
Published:
2023-07-04
摘要:
在全基因组水平鉴定胡萝卜(Daucus carota)、芹菜(Apium graveolens)、香菜(Coriandrum sativum)3种伞形科蔬菜作物的棕榈酰基转移酶(protein S-acyltransferase, PAT),对其家族成员进行生物信息学和表达分析,为深入探究该类蛋白在3种伞形科作物生长发育及抵御逆境胁迫中的作用提供参考。利用Basic Local Alignment Search Tool(BLAST)和hmmsearch程序,从胡萝卜、芹菜、香菜基因组中筛选、鉴定PAT家族成员;利用TBtools绘制PAT基因染色体分布图;利用Expasy分析PAT蛋白的等电点、分子量;利用TMHMM-2.0预测PAT蛋白跨膜区;利用CELLO v.2.5、WoLF PSORT预测PAT蛋白的亚细胞定位情况;利用MEGA6构建系统发育进化树;利用MEME分析PAT蛋白保守基序;基于荧光定量PCR实验结果及转录组数据,利用TBtools绘制基因表达热图,分析PAT基因的组织表达特性及胁迫响应情况。结果表明,在胡萝卜、芹菜及香菜基因组中各存在27、25、27个PAT基因,分别命名为DcPAT1~27、AgPAT1~25、CsPAT1~27。这些PAT成员不均匀地分布于染色体上,理化性质存在差异,且均为跨膜蛋白。系统进化分析将伞形科及来源于其他物种的PAT蛋白分为7个类群(Group1~7),伞形科作物PAT蛋白在7个类群中均有分布。结构分析表明,所有的PAT蛋白均含有DHHC-CRD结构域,该序列内的保守氨基酸残基在不同物种间已发生变异。荧光定量PCR和转录组数据分析表明,PAT基因在胡萝卜、芹菜、香菜不同组织间的表达水平有差异,但有部分成员在不同组织间均呈现高水平或低水平表达,另有部分DcPAT基因在盐胁迫和低温胁迫处理后,表达水平发生改变。
中图分类号:
张新业, 李文静, 朱姝, 孙艳香, 王聪艳, 闫训友, 周志国. 三种伞形科蔬菜作物棕榈酰基转移酶基因家族的鉴定与分析[J]. 浙江农业学报, 2023, 35(6): 1315-1327.
ZHANG Xinye, LI Wenjing, ZHU Shu, SUN Yanxiang, WANG Congyan, YAN Xunyou, ZHOU Zhiguo. Identification and analysis of PAT gene family in three kinds of Apiaceae vegetable crops[J]. Acta Agriculturae Zhejiangensis, 2023, 35(6): 1315-1327.
基因名称 Gene ID | 正向引物 Sequence of forward primer (5'→3') | 反向引物 Sequence of revere primer (5'→3') | 退火温度 Annealing temperature/℃ |
---|---|---|---|
DcPAT10 | CATGTATGGATATCAGGCAACACA | TGGAACGTCTGGATCTGCAT | 60 |
DcPAT13 | CACTTGCATGCTTTACCGCC | AGTACCGGTAGTTGCGCTGT | 60 |
DcPAT17 | CATGGAAGGGCAGCAATGTTT | GCCACAAAAGATGGCTACAGG | 60 |
DcPAT20 | ACAAGTGTCCTTTGGCTCCT | TGCAAATGGAGCACGAGAGA | 63 |
DcPAT25 | ATGCAGGCACAAAGGAGGAA | ATGCTGCGCTCTAAGAAGGA | 60 |
DcActin | CGGTATTGTGTTGGACTCTGGTGAT | CAGCAAGGTCAAGACGGAGTATGG | 60/63 |
表1 引物信息
Table 1 Primers information
基因名称 Gene ID | 正向引物 Sequence of forward primer (5'→3') | 反向引物 Sequence of revere primer (5'→3') | 退火温度 Annealing temperature/℃ |
---|---|---|---|
DcPAT10 | CATGTATGGATATCAGGCAACACA | TGGAACGTCTGGATCTGCAT | 60 |
DcPAT13 | CACTTGCATGCTTTACCGCC | AGTACCGGTAGTTGCGCTGT | 60 |
DcPAT17 | CATGGAAGGGCAGCAATGTTT | GCCACAAAAGATGGCTACAGG | 60 |
DcPAT20 | ACAAGTGTCCTTTGGCTCCT | TGCAAATGGAGCACGAGAGA | 63 |
DcPAT25 | ATGCAGGCACAAAGGAGGAA | ATGCTGCGCTCTAAGAAGGA | 60 |
DcActin | CGGTATTGTGTTGGACTCTGGTGAT | CAGCAAGGTCAAGACGGAGTATGG | 60/63 |
基因名称 Gene ID | 蛋白ID Protein ID | 基因长度 Gene length/bp | 外显子数目 Number of exon | 氨基酸数目 Number of amino acids/aa | 蛋白分子量 Molecular weight/ku | 等电点 Isoelectric point | 跨膜区个数 Number of TMD | 亚细胞定位 Subcellular localization |
---|---|---|---|---|---|---|---|---|
DcPAT1 | XP_017231957.1 | 5 544 | 10 | 391 | 44.78 | 8.91 | 6 | 质膜Plasma membrane |
DcPAT2 | XP_017232727.1 | 3 079 | 9 | 505 | 57.64 | 9.40 | 5 | 质膜Plasma membrane |
DcPAT3 | XP_017234967.1 | 5 170 | 7 | 281 | 31.97 | 9.12 | 4 | 质膜Plasma membrane |
DcPAT4 | XP_017231515.1 | 5 299 | 7 | 307 | 34.39 | 6.65 | 4 | 质膜Plasma membrane |
DcPAT5 | XP_017235454.1 | 5 088 | 9 | 627 | 69.43 | 8.92 | 4 | 质膜Plasma membrane |
DcPAT6 | XP_017234510.1 | 3 110 | 5 | 418 | 46.43 | 6.88 | 4 | 质膜Plasma membrane |
DcPAT7 | XP_017231049.1 | 4 286 | 7 | 307 | 34.65 | 6.13 | 4 | 质膜Plasma membrane |
DcPAT8 | XP_017241016.1 | 4 035 | 5 | 407 | 46.85 | 9.43 | 4 | 质膜Plasma membrane |
DcPAT9 | XP_017237722.1 | 5 752 | 5 | 413 | 47.87 | 6.17 | 4 | 质膜Plasma membrane |
DcPAT10 | XP_017242796.1 | 12 229 | 13 | 624 | 68.66 | 6.64 | 4 | 质膜Plasma membrane |
DcPAT11 | XP_017242953.1 | 8 840 | 11 | 346 | 39.90 | 8.79 | 4 | 质膜Plasma membrane |
DcPAT12 | XP_017248113.1 | 6 759 | 13 | 632 | 69.45 | 6.41 | 4 | 质膜Plasma membrane |
DcPAT13 | XP_017247520.1 | 6 889 | 5 | 424 | 48.71 | 7.57 | 4 | 质膜Plasma membrane |
DcPAT14 | XP_017246994.1 | 6 851 | 10 | 633 | 69.33 | 6.80 | 4 | 质膜Plasma membrane |
DcPAT15 | XP_017245764.1 | 2 421 | 5 | 401 | 45.12 | 9.47 | 4 | 质膜Plasma membrane |
DcPAT16 | XP_017246280.1 | 6 834 | 9 | 653 | 71.33 | 8.10 | 2 | 质膜Plasma membrane |
DcPAT17 | XP_017253762.1 | 5 261 | 5 | 441 | 49.57 | 8.22 | 4 | 质膜Plasma membrane |
DcPAT18 | XP_017252794.1 | 4 594 | 4 | 345 | 39.35 | 8.99 | 3 | 质膜Plasma membrane |
DcPAT19 | XP_017249993.1 | 4 864 | 12 | 344 | 38.59 | 8.60 | 6 | 质膜Plasma membrane |
DcPAT20 | XP_017258526.1 | 9 185 | 10 | 731 | 79.22 | 8.58 | 4 | 质膜Plasma membrane |
DcPAT21 | XP_017214813.1 | 5 759 | 5 | 447 | 49.99 | 8.91 | 4 | 质膜Plasma membrane |
DcPAT22 | XP_017254573.1 | 5 903 | 5 | 439 | 49.30 | 8.78 | 4 | 质膜Plasma membrane |
DcPAT23 | XP_017216415.1 | 4 688 | 4 | 356 | 39.87 | 9.10 | 4 | 质膜Plasma membrane |
DcPAT24 | XP_017220920.1 | 3 730 | 7 | 277 | 31.20 | 8.25 | 4 | 质膜Plasma membrane |
DcPAT25 | XP_017226581.1 | 10 201 | 13 | 538 | 59.90 | 6.30 | 4 | 质膜Plasma membrane |
DcPAT26 | XP_017224127.1 | 5 827 | 4 | 364 | 40.54 | 7.46 | 4 | 质膜Plasma membrane |
DcPAT27 | XP_017224090.1 | 5 681 | 9 | 627 | 69.16 | 8.56 | 4 | 质膜Plasma membrane |
AgPAT1 | Ag1G00678.1 | 16 983 | 14 | 566 | 63.21 | 6.48 | 4 | 质膜Plasma membrane |
AgPAT2 | Ag1G01444.1 | 5 264 | 9 | 627 | 69.42 | 8.56 | 4 | 质膜Plasma membrane |
AgPAT3 | Ag2G01570.1 | 1 651 | 3 | 330 | 37.67 | 8.41 | 2 | 质膜Plasma membrane |
AgPAT4 | Ag3G00580.1 | 5 851 | 9 | 725 | 79.76 | 8.66 | 4 | 质膜Plasma membrane |
AgPAT5 | Ag3G01929.1 | 6 092 | 5 | 424 | 48.77 | 8.35 | 4 | 质膜Plasma membrane |
AgPAT6 | Ag3G02080.1 | 9 965 | 11 | 348 | 40.26 | 8.80 | 4 | 质膜Plasma membrane |
AgPAT7 | Ag3G02578.1 | 6 182 | 10 | 631 | 69.41 | 7.54 | 4 | 质膜Plasma membrane |
AgPAT8 | Ag3G02732.1 | 11 809 | 13 | 631 | 69.47 | 6.31 | 4 | 质膜Plasma membrane |
AgPAT9 | Ag4G00005.1 | 5 085 | 5 | 441 | 49.62 | 7.53 | 4 | 质膜Plasma membrane |
AgPAT10 | Ag4G01427.1 | 5 150 | 5 | 398 | 45.72 | 9.20 | 4 | 质膜Plasma membrane |
AgPAT11 | Ag4G01428.1 | 6 853 | 6 | 413 | 48.01 | 6.02 | 4 | 质膜Plasma membrane |
AgPAT12 | Ag4G01654.1 | 19 854 | 13 | 625 | 68.63 | 6.49 | 4 | 质膜Plasma membrane |
AgPAT13 | Ag5G01814.1 | 4 227 | 3 | 230 | 25.85 | 9.29 | 2 | 质膜Plasma membrane |
AgPAT14 | Ag6G00122.1 | 4 968 | 7 | 307 | 34.52 | 5.98 | 4 | 质膜Plasma membrane |
AgPAT15 | Ag6G00420.1 | 5 916 | 10 | 626 | 69.17 | 9.00 | 4 | 质膜Plasma membrane |
AgPAT16 | Ag6G01499.1 | 3 608 | 5 | 384 | 43.87 | 8.36 | 4 | 质膜Plasma membrane |
AgPAT17 | Ag6G01663.1 | 6 690 | 7 | 307 | 34.59 | 6.65 | 4 | 质膜Plasma membrane |
AgPAT18 | Ag6G02949.1 | 11 924 | 10 | 392 | 45.05 | 9.08 | 6 | 质膜Plasma membrane |
AgPAT19 | Ag8G00168.1 | 3 545 | 7 | 277 | 31.27 | 8.38 | 4 | 质膜Plasma membrane |
AgPAT20 | Ag9G01243.1 | 5 048 | 5 | 437 | 49.33 | 8.68 | 4 | 质膜Plasma membrane |
AgPAT21 | Ag11G03538.1 | 11 445 | 9 | 727 | 78.74 | 8.74 | 4 | 质膜Plasma membrane |
AgPAT22 | Ag11G03938.1 | 2 801 | 9 | 500 | 57.00 | 9.55 | 5 | 质膜Plasma membrane |
AgPAT23 | AgUnG00368.1 | 5 493 | 5 | 443 | 49.67 | 8.83 | 4 | 质膜Plasma membrane |
AgPAT24 | AgUnG01143.1 | 11 544 | 13 | 631 | 69.42 | 6.31 | 4 | 质膜Plasma membrane |
AgPAT25 | AgUnG01289.1 | 4 578 | 7 | 283 | 32.37 | 9.15 | 4 | 质膜Plasma membrane |
CsPAT1 | Cs01G00487.1 | 3 053 | 9 | 504 | 57.63 | 9.45 | 4 | 质膜Plasma membrane |
CsPAT2 | Cs01G01377.1 | 4 658 | 5 | 441 | 49.57 | 7.95 | 4 | 质膜Plasma membrane |
CsPAT3 | Cs02G01000.1 | 5 130 | 10 | 250 | 27.89 | 8.68 | 3 | 质膜Plasma membrane |
CsPAT4 | Cs03G02877.1 | 4 546 | 5 | 413 | 48.07 | 6.47 | 4 | 质膜Plasma membrane |
CsPAT5 | Cs03G02879.1 | 3 500 | 5 | 408 | 47.25 | 9.36 | 4 | 质膜Plasma membrane |
CsPAT6 | Cs04G00058.1 | 6 781 | 9 | 720 | 78.10 | 8.68 | 4 | 质膜Plasma membrane |
CsPAT7 | Cs05G00045.1 | 4 286 | 9 | 277 | 31.19 | 8.56 | 4 | 质膜Plasma membrane |
CsPAT8 | Cs07G02759.1 | 4 664 | 4 | 361 | 40.52 | 8.80 | 5 | 质膜Plasma membrane |
CsPAT9 | Cs09G00033.1 | 9 462 | 10 | 392 | 44.93 | 9.08 | 6 | 质膜Plasma membrane |
CsPAT10 | Cs09G01589.1 | 6 526 | 7 | 307 | 34.43 | 7.02 | 4 | 质膜Plasma membrane |
CsPAT11 | Cs09G02975.1 | 4 906 | 9 | 625 | 68.98 | 9.02 | 4 | 质膜Plasma membrane |
CsPAT12 | Cs09G03315.1 | 4 262 | 7 | 305 | 34.25 | 6.44 | 4 | 质膜Plasma membrane |
CsPAT13 | Cs09G04864.1 | 6 143 | 9 | 431 | 47.63 | 8.77 | 4 | 质膜Plasma membrane |
CsPAT14 | Cs09G05377.1 | 5 760 | 9 | 627 | 69.60 | 8.64 | 4 | 质膜Plasma membrane |
CsPAT15 | Cs10G00189.1 | 3 010 | 5 | 401 | 45.75 | 8.91 | 4 | 质膜Plasma membrane |
CsPAT16 | Cs10G00720.1 | 8 155 | 5 | 371 | 43.08 | 6.82 | 3 | 质膜Plasma membrane |
CsPAT17 | Cs10G01251.1 | 13 537 | 11 | 350 | 40.41 | 8.67 | 4 | 质膜Plasma membrane |
CsPAT18 | Cs10G01544.1 | 6 303 | 10 | 631 | 69.68 | 8.03 | 4 | 质膜Plasma membrane |
CsPAT19 | Cs10G01658.1 | 7 185 | 13 | 630 | 69.20 | 6.44 | 4 | 质膜Plasma membrane |
CsPAT20 | Cs10G01915.1 | 5 199 | 5 | 438 | 49.44 | 8.79 | 4 | 质膜Plasma membrane |
CsPAT21 | Cs10G02331.1 | 5 362 | 5 | 446 | 49.85 | 8.82 | 4 | 质膜Plasma membrane |
CsPAT22 | Cs10G03215.1 | 3 461 | 5 | 308 | 35.43 | 8.81 | 4 | 质膜Plasma membrane |
CsPAT23 | Cs11G00778.1 | 7 628 | 13 | 538 | 59.78 | 6.27 | 4 | 质膜Plasma membrane |
CsPAT24 | Cs11G01042.1 | 4 192 | 4 | 364 | 40.58 | 6.77 | 5 | 质膜Plasma membrane |
CsPAT25 | CsUnG00290.1 | 2 050 | 4 | 358 | 41.33 | 8.66 | 3 | 质膜Plasma membrane |
CsPAT26 | CsUnG01718.1 | 3 463 | 5 | 308 | 35.42 | 8.81 | 4 | 质膜Plasma membrane |
CsPAT27 | CsUnG03874.1 | 3 495 | 5 | 411 | 47.34 | 8.98 | 4 | 质膜Plasma membrane |
表2 胡萝卜、香菜、芹菜PAT基因家族成员信息
Table 2 Information of PAT gene family members in Daucus carota, Apium graveolens and Coriandrum sativum
基因名称 Gene ID | 蛋白ID Protein ID | 基因长度 Gene length/bp | 外显子数目 Number of exon | 氨基酸数目 Number of amino acids/aa | 蛋白分子量 Molecular weight/ku | 等电点 Isoelectric point | 跨膜区个数 Number of TMD | 亚细胞定位 Subcellular localization |
---|---|---|---|---|---|---|---|---|
DcPAT1 | XP_017231957.1 | 5 544 | 10 | 391 | 44.78 | 8.91 | 6 | 质膜Plasma membrane |
DcPAT2 | XP_017232727.1 | 3 079 | 9 | 505 | 57.64 | 9.40 | 5 | 质膜Plasma membrane |
DcPAT3 | XP_017234967.1 | 5 170 | 7 | 281 | 31.97 | 9.12 | 4 | 质膜Plasma membrane |
DcPAT4 | XP_017231515.1 | 5 299 | 7 | 307 | 34.39 | 6.65 | 4 | 质膜Plasma membrane |
DcPAT5 | XP_017235454.1 | 5 088 | 9 | 627 | 69.43 | 8.92 | 4 | 质膜Plasma membrane |
DcPAT6 | XP_017234510.1 | 3 110 | 5 | 418 | 46.43 | 6.88 | 4 | 质膜Plasma membrane |
DcPAT7 | XP_017231049.1 | 4 286 | 7 | 307 | 34.65 | 6.13 | 4 | 质膜Plasma membrane |
DcPAT8 | XP_017241016.1 | 4 035 | 5 | 407 | 46.85 | 9.43 | 4 | 质膜Plasma membrane |
DcPAT9 | XP_017237722.1 | 5 752 | 5 | 413 | 47.87 | 6.17 | 4 | 质膜Plasma membrane |
DcPAT10 | XP_017242796.1 | 12 229 | 13 | 624 | 68.66 | 6.64 | 4 | 质膜Plasma membrane |
DcPAT11 | XP_017242953.1 | 8 840 | 11 | 346 | 39.90 | 8.79 | 4 | 质膜Plasma membrane |
DcPAT12 | XP_017248113.1 | 6 759 | 13 | 632 | 69.45 | 6.41 | 4 | 质膜Plasma membrane |
DcPAT13 | XP_017247520.1 | 6 889 | 5 | 424 | 48.71 | 7.57 | 4 | 质膜Plasma membrane |
DcPAT14 | XP_017246994.1 | 6 851 | 10 | 633 | 69.33 | 6.80 | 4 | 质膜Plasma membrane |
DcPAT15 | XP_017245764.1 | 2 421 | 5 | 401 | 45.12 | 9.47 | 4 | 质膜Plasma membrane |
DcPAT16 | XP_017246280.1 | 6 834 | 9 | 653 | 71.33 | 8.10 | 2 | 质膜Plasma membrane |
DcPAT17 | XP_017253762.1 | 5 261 | 5 | 441 | 49.57 | 8.22 | 4 | 质膜Plasma membrane |
DcPAT18 | XP_017252794.1 | 4 594 | 4 | 345 | 39.35 | 8.99 | 3 | 质膜Plasma membrane |
DcPAT19 | XP_017249993.1 | 4 864 | 12 | 344 | 38.59 | 8.60 | 6 | 质膜Plasma membrane |
DcPAT20 | XP_017258526.1 | 9 185 | 10 | 731 | 79.22 | 8.58 | 4 | 质膜Plasma membrane |
DcPAT21 | XP_017214813.1 | 5 759 | 5 | 447 | 49.99 | 8.91 | 4 | 质膜Plasma membrane |
DcPAT22 | XP_017254573.1 | 5 903 | 5 | 439 | 49.30 | 8.78 | 4 | 质膜Plasma membrane |
DcPAT23 | XP_017216415.1 | 4 688 | 4 | 356 | 39.87 | 9.10 | 4 | 质膜Plasma membrane |
DcPAT24 | XP_017220920.1 | 3 730 | 7 | 277 | 31.20 | 8.25 | 4 | 质膜Plasma membrane |
DcPAT25 | XP_017226581.1 | 10 201 | 13 | 538 | 59.90 | 6.30 | 4 | 质膜Plasma membrane |
DcPAT26 | XP_017224127.1 | 5 827 | 4 | 364 | 40.54 | 7.46 | 4 | 质膜Plasma membrane |
DcPAT27 | XP_017224090.1 | 5 681 | 9 | 627 | 69.16 | 8.56 | 4 | 质膜Plasma membrane |
AgPAT1 | Ag1G00678.1 | 16 983 | 14 | 566 | 63.21 | 6.48 | 4 | 质膜Plasma membrane |
AgPAT2 | Ag1G01444.1 | 5 264 | 9 | 627 | 69.42 | 8.56 | 4 | 质膜Plasma membrane |
AgPAT3 | Ag2G01570.1 | 1 651 | 3 | 330 | 37.67 | 8.41 | 2 | 质膜Plasma membrane |
AgPAT4 | Ag3G00580.1 | 5 851 | 9 | 725 | 79.76 | 8.66 | 4 | 质膜Plasma membrane |
AgPAT5 | Ag3G01929.1 | 6 092 | 5 | 424 | 48.77 | 8.35 | 4 | 质膜Plasma membrane |
AgPAT6 | Ag3G02080.1 | 9 965 | 11 | 348 | 40.26 | 8.80 | 4 | 质膜Plasma membrane |
AgPAT7 | Ag3G02578.1 | 6 182 | 10 | 631 | 69.41 | 7.54 | 4 | 质膜Plasma membrane |
AgPAT8 | Ag3G02732.1 | 11 809 | 13 | 631 | 69.47 | 6.31 | 4 | 质膜Plasma membrane |
AgPAT9 | Ag4G00005.1 | 5 085 | 5 | 441 | 49.62 | 7.53 | 4 | 质膜Plasma membrane |
AgPAT10 | Ag4G01427.1 | 5 150 | 5 | 398 | 45.72 | 9.20 | 4 | 质膜Plasma membrane |
AgPAT11 | Ag4G01428.1 | 6 853 | 6 | 413 | 48.01 | 6.02 | 4 | 质膜Plasma membrane |
AgPAT12 | Ag4G01654.1 | 19 854 | 13 | 625 | 68.63 | 6.49 | 4 | 质膜Plasma membrane |
AgPAT13 | Ag5G01814.1 | 4 227 | 3 | 230 | 25.85 | 9.29 | 2 | 质膜Plasma membrane |
AgPAT14 | Ag6G00122.1 | 4 968 | 7 | 307 | 34.52 | 5.98 | 4 | 质膜Plasma membrane |
AgPAT15 | Ag6G00420.1 | 5 916 | 10 | 626 | 69.17 | 9.00 | 4 | 质膜Plasma membrane |
AgPAT16 | Ag6G01499.1 | 3 608 | 5 | 384 | 43.87 | 8.36 | 4 | 质膜Plasma membrane |
AgPAT17 | Ag6G01663.1 | 6 690 | 7 | 307 | 34.59 | 6.65 | 4 | 质膜Plasma membrane |
AgPAT18 | Ag6G02949.1 | 11 924 | 10 | 392 | 45.05 | 9.08 | 6 | 质膜Plasma membrane |
AgPAT19 | Ag8G00168.1 | 3 545 | 7 | 277 | 31.27 | 8.38 | 4 | 质膜Plasma membrane |
AgPAT20 | Ag9G01243.1 | 5 048 | 5 | 437 | 49.33 | 8.68 | 4 | 质膜Plasma membrane |
AgPAT21 | Ag11G03538.1 | 11 445 | 9 | 727 | 78.74 | 8.74 | 4 | 质膜Plasma membrane |
AgPAT22 | Ag11G03938.1 | 2 801 | 9 | 500 | 57.00 | 9.55 | 5 | 质膜Plasma membrane |
AgPAT23 | AgUnG00368.1 | 5 493 | 5 | 443 | 49.67 | 8.83 | 4 | 质膜Plasma membrane |
AgPAT24 | AgUnG01143.1 | 11 544 | 13 | 631 | 69.42 | 6.31 | 4 | 质膜Plasma membrane |
AgPAT25 | AgUnG01289.1 | 4 578 | 7 | 283 | 32.37 | 9.15 | 4 | 质膜Plasma membrane |
CsPAT1 | Cs01G00487.1 | 3 053 | 9 | 504 | 57.63 | 9.45 | 4 | 质膜Plasma membrane |
CsPAT2 | Cs01G01377.1 | 4 658 | 5 | 441 | 49.57 | 7.95 | 4 | 质膜Plasma membrane |
CsPAT3 | Cs02G01000.1 | 5 130 | 10 | 250 | 27.89 | 8.68 | 3 | 质膜Plasma membrane |
CsPAT4 | Cs03G02877.1 | 4 546 | 5 | 413 | 48.07 | 6.47 | 4 | 质膜Plasma membrane |
CsPAT5 | Cs03G02879.1 | 3 500 | 5 | 408 | 47.25 | 9.36 | 4 | 质膜Plasma membrane |
CsPAT6 | Cs04G00058.1 | 6 781 | 9 | 720 | 78.10 | 8.68 | 4 | 质膜Plasma membrane |
CsPAT7 | Cs05G00045.1 | 4 286 | 9 | 277 | 31.19 | 8.56 | 4 | 质膜Plasma membrane |
CsPAT8 | Cs07G02759.1 | 4 664 | 4 | 361 | 40.52 | 8.80 | 5 | 质膜Plasma membrane |
CsPAT9 | Cs09G00033.1 | 9 462 | 10 | 392 | 44.93 | 9.08 | 6 | 质膜Plasma membrane |
CsPAT10 | Cs09G01589.1 | 6 526 | 7 | 307 | 34.43 | 7.02 | 4 | 质膜Plasma membrane |
CsPAT11 | Cs09G02975.1 | 4 906 | 9 | 625 | 68.98 | 9.02 | 4 | 质膜Plasma membrane |
CsPAT12 | Cs09G03315.1 | 4 262 | 7 | 305 | 34.25 | 6.44 | 4 | 质膜Plasma membrane |
CsPAT13 | Cs09G04864.1 | 6 143 | 9 | 431 | 47.63 | 8.77 | 4 | 质膜Plasma membrane |
CsPAT14 | Cs09G05377.1 | 5 760 | 9 | 627 | 69.60 | 8.64 | 4 | 质膜Plasma membrane |
CsPAT15 | Cs10G00189.1 | 3 010 | 5 | 401 | 45.75 | 8.91 | 4 | 质膜Plasma membrane |
CsPAT16 | Cs10G00720.1 | 8 155 | 5 | 371 | 43.08 | 6.82 | 3 | 质膜Plasma membrane |
CsPAT17 | Cs10G01251.1 | 13 537 | 11 | 350 | 40.41 | 8.67 | 4 | 质膜Plasma membrane |
CsPAT18 | Cs10G01544.1 | 6 303 | 10 | 631 | 69.68 | 8.03 | 4 | 质膜Plasma membrane |
CsPAT19 | Cs10G01658.1 | 7 185 | 13 | 630 | 69.20 | 6.44 | 4 | 质膜Plasma membrane |
CsPAT20 | Cs10G01915.1 | 5 199 | 5 | 438 | 49.44 | 8.79 | 4 | 质膜Plasma membrane |
CsPAT21 | Cs10G02331.1 | 5 362 | 5 | 446 | 49.85 | 8.82 | 4 | 质膜Plasma membrane |
CsPAT22 | Cs10G03215.1 | 3 461 | 5 | 308 | 35.43 | 8.81 | 4 | 质膜Plasma membrane |
CsPAT23 | Cs11G00778.1 | 7 628 | 13 | 538 | 59.78 | 6.27 | 4 | 质膜Plasma membrane |
CsPAT24 | Cs11G01042.1 | 4 192 | 4 | 364 | 40.58 | 6.77 | 5 | 质膜Plasma membrane |
CsPAT25 | CsUnG00290.1 | 2 050 | 4 | 358 | 41.33 | 8.66 | 3 | 质膜Plasma membrane |
CsPAT26 | CsUnG01718.1 | 3 463 | 5 | 308 | 35.42 | 8.81 | 4 | 质膜Plasma membrane |
CsPAT27 | CsUnG03874.1 | 3 495 | 5 | 411 | 47.34 | 8.98 | 4 | 质膜Plasma membrane |
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