辣椒抗番茄斑萎病毒研究进展

doi:10.3969/j.issn.1004-1524.20231156

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (10): 2416-2425.DOI: 10.3969/j.issn.1004-1524.20231156

辣椒抗番茄斑萎病毒研究进展

郭娜纳(), 李伟, 黄立娟, 张涛, 魏兵强^*()

甘肃农业大学园艺学院,甘肃兰州 730070

收稿日期:2023-10-07 出版日期:2024-10-25 发布日期:2024-10-30
作者简介:郭娜纳(2000—),女,甘肃平凉人,硕士研究生,研究方向为蔬菜遗传与分子育种。E-mail:g18093367179@163.com
通讯作者: ^*魏兵强,E-mail:bqwei@gsau.edu.cn
基金资助:
甘肃省重点研发计划(21YF5NA091);兰州市人才项目(2021-RC-65);甘肃农业大学青年导师扶持项目(GAU-KYQD-2018-17)

Research progress on resistance of pepper to Tomato spotted wilt virus (TSWV)

GUO Nana(), LI Wei, HUANG Lijuan, ZHANG Tao, WEI Bingqiang^*()

College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China

Received:2023-10-07 Online:2024-10-25 Published:2024-10-30

摘要/Abstract

摘要：

番茄斑萎病毒属(Tospovirus)是布尼亚病毒科(Bunyaviridae)中唯一能够侵染植物的病毒属,其核心成员番茄斑萎病毒(Tomato spotted wilt virus,TSWV)对全球农业构成了严峻挑战。TSWV作为一种负单链RNA型病毒,主要通过蓟马持久性增殖传播。辣椒受TSWV危害尤为严重,常表现为落叶、新叶黄化畸形、生长点坏死等多种复合症状,造成了巨大的经济损失。近年来,随着生物信息学研究的不断深入和辣椒全基因组测序的完成,研究者对TSWV的致病机理与辣椒抗TSWV机制的研究取得了较好的进展。PCR和逆转录PCR(RT-PCR)技术已被广泛应用于TSWV的精准检测,特别是针对核壳体蛋白基因(N-gene)设计的特异性引物,显著提升了检测效率与准确性。同时,抗性分子标记已广泛应用于TSWV抗性种质资源的检测与鉴定。选育抗病品种是防治TSWV最有效的途径,通过广泛筛选国内外抗性种质资源,并结合分子标记技术,已成功培育出中椒115等抗TSWV辣椒新品种。此外,TSWV还可通过抗原结构变异或宿主免疫抑制等免疫逃逸机制,成功规避植物防御系统,从而加剧防控难度。因此,未来研究需聚焦于抗性遗传资源的深度挖掘、育种技术体系的完善与新型抗性基因的发掘,以培育出更广泛、更持久抗性的辣椒新品种。

关键词: 辣椒, 番茄斑萎病毒, 抗性, 种质创新

Abstract:

Tospovirus, the only genus of viruses within the Bunyaviridae family that can invade plants, poses a significant challenge to global agriculture. Its primary member, Tomato spotted wilt virus (TSWV), is particularly detrimental to the pepper industry. TSWV, a negative single-stranded RNA virus, is primarily transmitted through the persistent proliferation of thrips. Peppers frequently exhibit a range of complex symptoms, including leaf shedding, yellowing of new leaves, and necrosis at the growth point when infected with TSWV. In recent years, as bioinformatics research has advanced and the whole-genome sequencing of pepper has been completed, researchers have made significant progress in understanding the pathogenesis of TSWV and the mechanisms of resistance to TSWV in pepper plants. In terms of TSWV detection, PCR and RT-PCR technologies have been widely used for the precise detection of TSWV, especially the specific primers designed for the nuclear capsid protein gene (N-gene), which have significantly improved the detection efficiency and accuracy. Concurrently, molecular markers associated with resistance have been extensively applied for detecting and characterizing germplasm resources resistant to TSWV. The most effective strategy for preventing and controlling TSWV is to develop disease-resistant varieties. New varieties of resistant to TSWV in pepper such as ‘Zhongjiao 115’ have been successfully cultivated via extensive screening of resistant germplasm resources at home and abroad, combined with molecular marker technology. In addition, the virus can effectively evade the plant defense system through immune evasion strategies, such as antigenic structural mutations or host immunosuppression, thereby increasing the challenge of prevention and control. So, future research needs to focus on the in-depth mining of resistance genetic resources, improvement of breeding technology system and discovery of novel resistance genes, in order to breed pepper varieties with broader and more durable resistance.

Key words: pepper, Tomato spotted wilt virus, germplasm innovation, resistance

中图分类号:

S436.418.12

郭娜纳, 李伟, 黄立娟, 张涛, 魏兵强. 辣椒抗番茄斑萎病毒研究进展[J]. 浙江农业学报, 2024, 36(10): 2416-2425.

GUO Nana, LI Wei, HUANG Lijuan, ZHANG Tao, WEI Bingqiang. Research progress on resistance of pepper to Tomato spotted wilt virus (TSWV)[J]. Acta Agriculturae Zhejiangensis, 2024, 36(10): 2416-2425.

图/表 2

参考文献 70

[1]	KABAŞ A, FIDAN H, BATUHAN DEMIRELLI M. Identification of new sources of resistance to resistance-breaking isolates of Tomato spotted wilt virus[J]. Saudi Journal of Biological Sciences, 2021, 28(5): 3094-3099.
[2]	WHITFIELD A E, KUMAR N K K, ROTENBERG D, et al. A soluble form of the Tomato spotted wilt virus(TSWV) glycoprotein G(N) (G(N)-S) inhibits transmission of TSWV by Frankliniella occidentalis[J]. Phytopathology, 2008, 98(1): 45-50.
[3]	LEE J D, HAN J H, YOON J B. A set of allele-specific markers linked to L locus resistant to tobamovirus in Capsicum spp[J]. Korean Journal of Horticultural Science and Technology, 2012, 30(3): 286-293.
[4]	PARRELLA G, GOGNALONS P, GEBRE-SELASSIÈ K, et al. An update of the host range of Tomato spotted wilt virus[J]. Journal of Plant Pathology, 2003, 85(4): 227-264.
[5]	DE ASSIS FILHO F M, DEOM C M, SHERWOOD J L. Acquisition of Tomato spotted wilt virus by adults of two Thrips species[J]. Phytopathology, 2004, 94(4): 333-336.
[6]	YANG H B, LIU W Y, KANG W H, et al. Development of SNP markers linked to the L locus in Capsicum spp. by a comparative genetic analysis[J]. Molecular Breeding, 2009, 24(4): 433-446.
[7]	习凤妮. 番茄斑萎病毒实时荧光定量PCR快速检测方法的建立[D]. 长沙: 中南大学, 2013.
	XI F N. Development of a real-time PCR for rapid detection and quantificantion of Tomato spotted wilt virus[D]. Changsha: Central South University, 2013. (in Chinese with English abstract)
[8]	ÇELIK I, ÖZALP R, ÇELIK N, et al. Development of long pepper (Capsicum annuum L.) lines resistant to Tomato spotted wilt virus(TSWV)[J]. Acta Horticulturae, 2020(1282): 37-42.
[9]	FERRAND L, ALMEIDA M M S, ORÍLIO A F, et al. Biological and molecular characterization of Tomato spotted wilt virus(TSWV) resistance-breaking isolates from Argentina[J]. Plant Pathology, 2019, 68(9): 1587-1601.
[10]	ZHU M, VAN GRINSVEN I L, KORMELINK R, et al. Paving the way to tospovirus infection: multilined interplays with plant innate immunity[J]. Annual Review of Phytopathology, 2019, 57: 41-62.
[11]	张振甲. 番茄斑萎病毒(TSWV)的分子进化及其亚基因组翻译调控的研究[D]. 泰安: 山东农业大学, 2016.
	ZHANG Z J. Evolutional analysis of Tomoto spotted wilt virus and translational characteristics of its subgenomic RNAs[D]. Taian: Shandong Agricultural University, 2016. (in Chinese with English abstract)
[12]	MANDAL B, MANDAL S, CSINOS A S, et al. Biological and molecular analyses of the acibenzolar-S-methyl-induced systemic acquired resistance in flue-cured tobacco against Tomato spotted wilt virus[J]. Phytopathology, 2008, 98(2): 196-204.
[13]	POLAT I, CELIK I, CELIK N, et al. Biological and molecular determination for resistance to Tomato spotted wilt virus(TSWV) in F₂ population of long-type pepper (Capsicum annuum L.)[J]. Acta Horticulturae, 2016(1145): 115-120.
[14]	HAN J H, LEE W P, LEE J D, et al. Symptom and resistance of cultivated and wild Capsicum accessions to Tomato spotted wilt virus[J]. Research in Plant Disease, 2011, 17(1): 59-65.
[15]	QI S M, ZHANG S J, ISLAM M M, et al. Natural resources resistance to Tomato spotted wilt virus(TSWV) in tomato (Solanum lycopersicum)[J]. International Journal of Molecular Sciences, 2021, 22(20): 10978.
[16]	雷蕾, 林清, 吕中华, 等. 重庆市辣椒病毒病毒原种类鉴定及株系确认[J]. 西南园艺, 2001(2): 28-29.
	LEI L, LIN Q, LÜ Z H, et al. Virus species and its strans identification of chilli virus diseases in Chongqing[J]. Southwest Horticulture, 2001(2): 28-29. (in Chinese)
[17]	林清, 吕中华, 黄任中, 等. 重庆市辣椒TMV和CMV的鉴定[J]. 中国蔬菜, 2001(3): 13-15.
	LIN Q, LÜ Z H, HUANG R Z, et al. Identification of TMV and CMV in peper in Chongqing area[J]. China Vegetables, 2001(3): 13-15. (in Chinese with English abstract)
[18]	刘勇, 李媛媛, 吴竹妍, 等. 广州辣椒上番茄斑萎病毒属病毒的鉴定[J]. 植物保护学报, 2010, 37(4): 383-384.
	LIU Y, LI Y Y, WU Z Y, et al. Identification of tospovirus on pepper in Guangzhou[J]. Journal of Plant Protection, 2010, 37(4): 383-384. (in Chinese with English abstract)
[19]	吕要斌, 张治军, 吴青君, 等. 外来入侵害虫西花蓟马防控技术研究与示范[J]. 应用昆虫学报, 2011, 48(3): 488-496.
	LV Y B, ZHANG Z J, WU Q J, et al. Research progress of the monitoring, forecast and sustainable management of invasive alien pest Frankliniella occidentalis in China[J]. Chinese Journal of Applied Entomology, 2011, 48(3): 488-496. (in Chinese with English abstract)
[20]	郭思瑶, 童艳, 黄娅, 等. 重庆辣椒病毒病病原初步鉴定和分析[J]. 园艺学报, 2015, 42(2): 263-270.
	GUO S Y, TONG Y, HUANG Y, et al. Preliminary identification and analyses of viruses causing pepper virus disease in Chongqing, China[J]. Acta Horticulturae Sinica, 2015, 42(2): 263-270. (in Chinese with English abstract)
[21]	高苇, 王勇, 张春祥, 等. 天津地区辣椒病毒病调查及毒源种类初步鉴定[J]. 山东农业科学, 2016, 48(3): 91-94.
	GAO W, WANG Y, ZHANG C X, et al. Investigation and pathogen preliminary identification of pepper virus disease in Tianjin[J]. Shandong Agricultural Sciences, 2016, 48(3): 91-94. (in Chinese with English abstract)
[22]	刘佳, 陈东亮, 梁玉镯, 等. 北京辣椒和番茄上番茄斑萎病毒的鉴定[J]. 植物检疫, 2021, 35(2): 44-48.
	LIU J, CHEN D L, LIANG Y Z, et al. Identification of Tomato spotted wilt virus on Beijing pepper and tomato[J]. Plant Quarantine, 2021, 35(2): 44-48. (in Chinese with English abstract)
[23]	王昆, 张宝玺, 张正海, 等. 辣椒抗番茄斑萎病毒病研究进展及展望[J]. 中国蔬菜, 2019(12): 18-24.
	WANG K, ZHANG B X, ZHANG Z H, et al. Research progress and prospect on pepper resistance to Tomato spot wilt virus disease[J]. China Vegetables, 2019(12): 18-24. (in Chinese with English abstract)
[24]	TSENG Y C, TILLMAN B L, PENG Z, et al. Identification of major QTLs underlying Tomato spotted wilt virus resistance in peanut cultivar Florida-EP(TM) ‘113’[J]. BMC Genetics, 2016, 17(1): 128.
[25]	WANG J H, ZHANG S Y, GONG D, et al. First report of chilli ringspot virus on chili pepper in China[J]. Plant Disease, 2012, 96(3): 462.
[26]	ROTENBERG D, JACOBSON A L, SCHNEWEIS D J, et al. Thrips transmission of tospoviruses[J]. Current Opinion in Virology, 2015, 15: 80-89.
[27]	LI Y Y, TAN G L, XIAO L, et al. A multiyear survey and identification of pepper-and tomato-infecting viruses in Yunnan Province, China[J]. Frontiers in Microbiology, 2021, 12: 623875.
[28]	郝志军. 辣椒病毒病发生的特点及防治技术[J]. 蔬菜, 2012(6): 32-33.
	HAO Z J. Occurrence characteristics and control techniques of pepper virus disease[J]. Vegetables, 2012(6): 32-33. (in Chinese)
[29]	汤亚飞, 裴凡, 李正刚, 等. 基于小RNA深度测序技术鉴定侵染广东辣椒的病毒种类[J]. 中国农业科学, 2019, 52(13): 2256-2267.
	TANG Y F, PEI F, LI Z G, et al. Identification of viruses infecting peppers in Guangdong by small RNA deep sequencing[J]. Scientia Agricultura Sinica, 2019, 52(13): 2256-2267. (in Chinese with English abstract)
[30]	CHUNG B N, LEE J H, KANG B C, et al. HR-mediated defense response is overcome at high temperatures in Capsicum species[J]. The Plant Pathology Journal, 2018, 34(1): 71-77.
[31]	OCAMPO OCAMPO T, GABRIEL PERALTA S M, BACHELLER N, et al. Antiviral RNA silencing suppression activity of Tomato spotted wilt virus NSs protein[J]. Genetics and Molecular Research, 2016, 15(2): gmr8625.
[32]	BOITEUX L S. Allelic relationships between genes for resistance to Tomato spotted wilt tospovirus in Capsicum chinense[J]. TAG Theoretical and Applied Genetics Theoretische und Angewandte Genetik, 1995, 90(1): 146-149.
[33]	NGOC HUY, HOANG, et al. Molecular mapping of the Tsw locus for resistance to Tomato spotted wilt virus(TSWV) in Capsicum[J]. Horticulture Abstracts, 2012:101-102.
[34]	OCHAR K, KO H C, WOO H J, et al. Pepper mild mottle virus: an infectious pathogen in pepper production and a potential indicator of domestic water quality[J]. Viruses, 2023, 15(2): 282.
[35]	蒋磊. 番茄免疫受体Sw-5b对Tospovirus广谱抗性的分子机制研究与我国TSWV Tsw抗性突破株系的遗传差异分析[D]. 南京: 南京农业大学, 2017.
	JIANG L. Molecular mechanism study of tomato immune receptor protein Sw-5b confers broad spectrum resistance to Tospovirus and genetic diversity analysis of Tsw resistance breaking isolates of TSWV in China[D]. Nanjing: Nanjing Agricultural University, 2017. (in Chinese with English abstract)
[36]	HATTA T, FRANCKI R I B. Cytopathic structures associated with tonoplasts of plant cells infected with cucumber mosaic and tomato aspermy viruses[J]. Journal of General Virology, 1981, 53(2): 343-346.
[37]	MARGARIA P, CIUFFO M, PACIFICO D, et al. Evidence that the nonstructural protein of Tomato spotted wilt virus is the avirulence determinant in the interaction with resistant pepper carrying the TSW gene[J]. Molecular Plant-Microbe Interactions, 2007, 20(5): 547-558.
[38]	ALMÁSI A, NEMES K, CSÖMÖR Z, et al. A single point mutation in Tomato spotted wilt virus NSs protein is sufficient to overcome Tsw-gene-mediated resistance in pepper[J]. The Journal of General Virology, 2017, 98(6): 1521-1525.
[39]	JIANG L, HUANG Y, SUN L, et al. Occurrence and diversity of Tomato spotted wilt virus isolates breaking the Tsw resistance gene of Capsicum chinense in Yunnan, Southwest China[J]. Plant Pathology, 2017, 66(6): 980-989.
[40]	桂敏, 高雪, 贾志强, 等. 云南辣椒正番茄斑萎病毒属病毒的分子鉴定[J]. 植物保护, 2022, 48(4): 286-292.
	GUI M, GAO X, JIA Z Q, et al. Molecular identification of Orthotospovirus on hot pepper in Yunnan[J]. Plant Protection, 2022, 48(4): 286-292. (in Chinese with English abstract)
[41]	付尚松. 贵州辣椒RNA病毒种类组成及株系变异研究[D]. 贵阳: 贵州大学, 2020.
	FU S S. Specises and strain variation of pepper RNA virus in Guizhou[D]. Guiyang: Guizhou University, 2020. (in Chinese with English abstract)
[42]	ALMÁSI A, CSILLÉRY G, CSÖMÖR Z, et al. Phylogenetic analysis of Tomato spotted wilt virus(TSWV) NSs protein demonstrates the isolated emergence of resistance-breaking strains in pepper[J]. Virus Genes, 2015, 50(1): 71-78.
[43]	DE RONDE D, BUTTERBACH P, LOHUIS D, et al. Tsw gene-based resistance is triggered by a functional RNA silencing suppressor protein of the Tomato spotted wilt virus[J]. Molecular Plant Pathology, 2013, 14(4): 405-415.
[44]	张学慧. 新疆辣椒病毒病病原鉴定及检测[D]. 石河子: 石河子大学, 2022.
	ZHANG X H. Pathogen identification and detection of pepper virus disease in Xinjiang[D]. Shihezi: Shihezi University, 2022. (in Chinese with English abstract)
[45]	吴淑华, 涂丽琴, 咸文荣, 等. 青海辣椒上番茄斑萎病毒检测及鉴定[J]. 园艺学报, 2020, 47(7): 1391-1400.
	WU S H, TU L Q, XIAN W R, et al. Detection and identification of Tomato spotted wilt tospovirus on pepper from Qinghai Province[J]. Acta Horticulturae Sinica, 2020, 47(7): 1391-1400. (in Chinese with English abstract)
[46]	NEMES K, SALÁNKI K. A multiplex RT-PCR assay for the simultaneous detection of prevalent viruses infecting pepper (Capsicum annuum L.)[J]. Journal of Virological Methods, 2020, 278: 113838.
[47]	NISCHWITZ C. New host and location reports of Tomato spotted wilt virus(TSWV) and Iris yellow spot virus(IYSV)[M]. Glasgow: Scottish Academic Press, 2006.
[48]	孙淼, 荆陈沉, 楚成茹, 等. 重庆辣椒上番茄斑萎病毒的血清学检测及分子鉴定[J]. 园艺学报, 2017, 44(3): 487-494.
	SUN M, JING C C, CHU C R, et al. Serological detection and molecular identification of Tomato spotted wilt virus in pepper in Chongqing[J]. Acta Horticulturae Sinica, 2017, 44(3): 487-494. (in Chinese with English abstract)
[49]	韩帅, 张河庆, 吴婕, 等. 侵染四川辣椒的番茄斑萎病毒和辣椒轻斑驳病毒的小RNA测序检测及鉴定[J]. 植物病理学报, 2020, 50(2): 147-154.
	HAN S, ZHANG H Q, WU J, et al. Identification and detection of Tomato spotted wilt virus and Pepper mild mottle virus infecting peppers in Sichuan using small RNA sequencing[J]. Acta Phytopathologica Sinica, 2020, 50(2): 147-154. (in Chinese with English abstract)
[50]	HEDIL M, KORMELINK R. Viral RNA silencing suppression: the Enigma of bunyavirus NSs proteins[J]. Viruses, 2016, 8(7): 208.
[51]	SCHNETTLER E, HEMMES H, HUISMANN R, et al. Diverging affinity of tospovirus RNA silencing suppressor proteins, NSs, for various RNA duplex molecules[J]. Journal of Virology, 2010, 84(21): 11542-11554.
[52]	ZHAI Y, GNANASEKARAN P, PAPPU H R. Identification and characterization of plant-interacting targets of Tomato spotted wilt virus silencing suppressor[J]. Pathogens, 2021, 10(1): 27.
[53]	CHEN J, ZHAO Y X, LUO X J, et al. NLR surveillance of pathogen interference with hormone receptors induces immunity[J]. Nature, 2023, 613(7942): 145-152.
[54]	TAO H Z, JIA Z Q, GAO X, et al. Analysis of the miRNA expression profile involved in the tomato spotted wilt Orthotospovirus-pepper interaction[J]. Virus Research, 2022, 312: 198710.
[55]	李洪磊. 番茄斑萎病毒的鉴定、Sw-5基因SSR标记的开发及抗病种质资源筛选[D]. 银川: 宁夏大学, 2021.
	LI H L. Identification of Tomato spotted wilt virus, development of SSR markers of Sw-5 gene and selection of resistant germplasm resources[D]. Yinchuan: Ningxia University, 2021. (in Chinese with English abstract)
[56]	KWON S J, CHO I S, YOON J Y, et al. Incidence and occurrence pattern of viruses on peppers growing in fields in Korea[J]. Research in Plant Disease, 2018, 24(1): 66-74.
[57]	于海龙, 靳远, 刘婧, 等. 我国辣椒病毒病发生情况及发展趋势: 基于2018年和2019年辣椒主产区的调查[J]. 中国蔬菜, 2020(9): 25-30.
	YU H L, JIN Y, LIU J, et al. Occurrence and development trend of pepper virus disease in China: based on main pepper producing areas investigation in 2018 and 2019[J]. China Vegetables, 2020(9): 25-30. (in Chinese with English abstract)
[58]	JAHN M, PARAN I, HOFFMANN K, et al. Genetic mapping of the Tsw locus for resistance to the Tospovirus Tomato spotted wilt virus in Capsicum spp. and its relationship to the Sw-5 gene for resistance to the same pathogen in tomato[J]. Molecular Plant-Microbe Interactions, 2000, 13(6): 673-682.
[59]	KIM S B, KANG W H, HUY H N, et al. Divergent evolution of multiple virus-resistance genes from a progenitor in Capsicum spp[J]. The New Phytologist, 2017, 213(2): 886-899.
[60]	MOURY B, PFLIEGER S, BLATTES A, et al. A CAPS marker to assist selection of Tomato spotted wilt virus(TSWV) resistance in pepper[J]. Genome, 2000, 43(1): 137-142.
[61]	KIM S, PARK M, YEOM S I, et al. Genome sequence of the hot pepper provides insights into the evolution of pungency in Capsicum species[J]. Nature Genetics, 2014, 46(3): 270-278.
[62]	LYU J F, YANG Y Y, SUN X H, et al. Genetic variability and molecular evolution of Tomato mosaic virus populations in three Northern China Provinces[J]. Viruses, 2023, 15(7): 1617.
[63]	THOMAS-CARROLL M L, JONES R C. Selection, biological properties and fitness of resistance-breaking strains of Tomato spotted wilt virus in pepper[J]. Annals of Applied Biology, 2003, 142(2): 235-243.
[64]	王立浩, 张正海, 毛胜利, 等. 甜椒抗番茄斑点萎蔫病毒的种质创新[J]. 中国蔬菜, 2016(2): 19-23.
	WANG L H, ZHANG Z H, MAO S L, et al. Innovation of sweet pepper (Capsicum annuum L.) germplasm resource with resistance to Tomato spots wilt virus[J]. China Vegetables, 2016(2): 19-23. (in Chinese with English abstract)
[65]	王立浩, 车晋英, 毛胜利, 等. 辣椒分子育种研究新进展[J]. 辣椒杂志, 2012, 10(4): 1-5.
	WANG L H, CHE J Y, MAO S L, et al. Review of molecular breeding research in hot pepper[J]. Journal of China Capsicum, 2012, 10(4): 1-5. (in Chinese with English abstract)
[66]	王立浩, 张宝玺, 张正海, 等. 抗番茄斑点萎蔫病毒甜椒新品种中椒115号的选育[J]. 中国蔬菜, 2019(4): 80-81.
	WANG L H, ZHANG B X, ZHANG Z H, et al. A sweet pepper variety resistant to Tomato spotted wilt virus: ‘Zhongjiao No.115’[J]. China Vegetables, 2019(4): 80-81. (in Chinese with English abstract)
[67]	HOANG N H, YANG H B, KANG B C. Identification and inheritance of a new source of resistance against Tomato spotted wilt virus(TSWV) in Capsicum[J]. Scientia Horticulturae, 2013, 161: 8-14.
[68]	李宁, 宫丽媛, 高升华, 等. 辣椒种质的抗性基因分子标记检测[J]. 中国蔬菜, 2020(8): 19-32.
	LI N, GONG L Y, GAO S H, et al. Detection of resistance gene of Capsicum spp. germplasm by molecular markers[J]. China Vegetables, 2020(8): 19-32. (in Chinese with English abstract)
[69]	SOLER S, DEBRECZENI D E, VIDAL E, et al. A new Capsicum baccatum accession shows tolerance to wild-type and resistance-breaking isolates of Tomato spotted wilt virus[J]. Annals of Applied Biology, 2015, 167(3): 343-353.
[70]	TURINA M, KORMELINK R, RESENDE R O. Resistance to Tospoviruses in vegetable crops: epidemiological and molecular aspects[J]. Annual Review of Phytopathology, 2016, 54: 347-371.

引物名称 Primer name	正向引物序列 Forward primer sequence (5’→3’)	反向引物序列 Reverse primer sequence(5’→3’)	片段大小 Fragment size/bp	参考文献 Literature
TSWV cp	ATGTCTAAGGTTAAGCTCAC	TCAAGCAAGTTCTGCGAGTT	777	[37]
TSNSs	GGCTGTAGCAGAGAGCAATTGTGTCATAATTTT	GGACATAGCAAGATTATTTTGATCCTG	1 404	[38]
TSWV	TTAAGCAAGTTCTGTGAG	ATGTCTAAGGTTAAGCTC	774	[39]
TSWV	ATGTYTAAGGTTAAGCTCACTAAG	TTAAGCAAGTTCTGTGAGTTTTGCC	750	[40]
TSWV cp	TCACTGTAATGTTCCATAGCAA	AGAGCAATTGTGTCATTTTATTC	839	[41]
TSWV-N	AATTTCTCCGCAATCTATTTCAGTTG	GGGGATCCAGAGCAATTGTGTCAATTTT	1 720	[42]
TSNSs	CCGTCGACATGTCTTCAAGTGTT	GGCGGCCGCTTATTTTGATCCTGAA	—	[43]
TSWV	CGCCCTAAGAAACGACTG	CAACAAGCCTGACCCTGA	406	[44]
TSWV	GCCATGGTCTTCTTCTGATGAA	AGTTATTGTCCCCTGACCCTTC	451	[45]
TSNSs	ATGTCTTCAAGTGTTTATGAGTC	TTATTTTGATCCTGAAGCATACG	1 401	[45]
TSWV	CCCAGCATTATGGCAAGCC	GGGCTAGCGGAAAACCTCGACCAGATCA	350	[46]
TSWV	TTAAGCAAGTTCTGTGAG	ATGTCTAAGGTTAAGCTC	774	[47]
TSWV cp	ATGTCTAAGGTTAAGCTCACTA	AGCAAGTTCTGCGAGTTTTGC	777	[47]
TSWV-J	GTTGTACTTTCGCACCT	GCATACTCTTTCCCTTTC	—	[48]
TSWV-N	TTAAGCAAGTTCTGCAAGTT	CATGTCTAAGGTTAAGCTCAC	777	[49]

引物名称 Primer name	正向引物序列 Forward primer sequence (5’→3’)	反向引物序列 Reverse primer sequence(5’→3’)	片段大小 Fragment size/bp	参考文献 Literature
TSWV cp	ATGTCTAAGGTTAAGCTCAC	TCAAGCAAGTTCTGCGAGTT	777	[37]
TSNSs	GGCTGTAGCAGAGAGCAATTGTGTCATAATTTT	GGACATAGCAAGATTATTTTGATCCTG	1 404	[38]
TSWV	TTAAGCAAGTTCTGTGAG	ATGTCTAAGGTTAAGCTC	774	[39]
TSWV	ATGTYTAAGGTTAAGCTCACTAAG	TTAAGCAAGTTCTGTGAGTTTTGCC	750	[40]
TSWV cp	TCACTGTAATGTTCCATAGCAA	AGAGCAATTGTGTCATTTTATTC	839	[41]
TSWV-N	AATTTCTCCGCAATCTATTTCAGTTG	GGGGATCCAGAGCAATTGTGTCAATTTT	1 720	[42]
TSNSs	CCGTCGACATGTCTTCAAGTGTT	GGCGGCCGCTTATTTTGATCCTGAA	—	[43]
TSWV	CGCCCTAAGAAACGACTG	CAACAAGCCTGACCCTGA	406	[44]
TSWV	GCCATGGTCTTCTTCTGATGAA	AGTTATTGTCCCCTGACCCTTC	451	[45]
TSNSs	ATGTCTTCAAGTGTTTATGAGTC	TTATTTTGATCCTGAAGCATACG	1 401	[45]
TSWV	CCCAGCATTATGGCAAGCC	GGGCTAGCGGAAAACCTCGACCAGATCA	350	[46]
TSWV	TTAAGCAAGTTCTGTGAG	ATGTCTAAGGTTAAGCTC	774	[47]
TSWV cp	ATGTCTAAGGTTAAGCTCACTA	AGCAAGTTCTGCGAGTTTTGC	777	[47]
TSWV-J	GTTGTACTTTCGCACCT	GCATACTCTTTCCCTTTC	—	[48]
TSWV-N	TTAAGCAAGTTCTGCAAGTT	CATGTCTAAGGTTAAGCTCAC	777	[49]

基因型 Genotype	连锁标记类型 Marker type	标记名称 Name	参考文献 Literature
Tsw	CAPS	SCAC₅₆₈	[58]
BC₄F₁	RAPD	Q-06₂₇₀	[58]
‘SP’	SNP	SNP246056-1	[59]
‘Telmo’F₂		SNP246056-2
		SNP246056-3
		SNP246056-4
		SNP653492-1
		SNP653492-2
		SNP653492-3
		2465-1
		1072-1
		7715-1
		17918-1
PI 195301×	RAPD	OPAC10	[60]
PI152225 F₂		OPAH13
		OPAF16
		OPB01
	CAPS	SCAC568	[60]

基因型 Genotype	连锁标记类型 Marker type	标记名称 Name	参考文献 Literature
Tsw	CAPS	SCAC₅₆₈	[58]
BC₄F₁	RAPD	Q-06₂₇₀	[58]
‘SP’	SNP	SNP246056-1	[59]
‘Telmo’F₂		SNP246056-2
		SNP246056-3
		SNP246056-4
		SNP653492-1
		SNP653492-2
		SNP653492-3
		2465-1
		1072-1
		7715-1
		17918-1
PI 195301×	RAPD	OPAC10	[60]
PI152225 F₂		OPAH13
		OPAF16
		OPB01
	CAPS	SCAC568	[60]

辣椒抗番茄斑萎病毒研究进展

Research progress on resistance of pepper to Tomato spotted wilt virus (TSWV)

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