浙江农业学报 ›› 2025, Vol. 37 ›› Issue (7): 1459-1468.DOI: 10.3969/j.issn.1004-1524.20240589
谈亚丽1(), 高梦祥1, 李晓洁2,*(
), 周英杰2, 熊健2, 曾子琦2, 李啸2, 杨华3
收稿日期:
2024-07-04
出版日期:
2025-07-25
发布日期:
2025-08-20
作者简介:
谈亚丽(1987—),女,湖北宜昌人,博士,高级工程师,研究方向为作物保护。E-mail:295312256@qq.com
通讯作者:
*李晓洁,E-mail:616451051@qq.com
基金资助:
TAN Yali1(), GAO Mengxiang1, LI Xiaojie2,*(
), ZHOU Yingjie2, XIONG Jian2, ZENG Ziqi2, LI Xiao2, YANG Hua3
Received:
2024-07-04
Online:
2025-07-25
Published:
2025-08-20
摘要:
为探究湖北省主栽核桃品种在不同采收期表观性状和营养组分的变化,以主栽品种秭林一号和清香核桃为研究对象,于4个不同采收期采收核桃,测定核桃的表观性状和营养组分,结合相关性分析和主成分分析法对4个不同采收期核桃的品质进行综合评价。结果表明,在采收期内,秭林一号和清香核桃的横径、纵径、出仁率和青果开裂率呈现不断升高的趋势。在营养组分方面,脂肪含量呈上升趋势,含水率、蛋白质含量和钙含量呈下降趋势,总氨基酸和锌含量则呈现先减少后增加的变化。相关性分析表明,各营养组分间的相关性较强。主成分分析显示,2个主成分代表了9个指标95.356%的变量信息,能全面反映核桃营养品质的整体变化,核桃品质的综合得分随着采收期的推迟明显升高,且在采收期Ⅳ达到最高。此外,适时推迟采收期能显著提高核桃的营养品质,湖北省主栽核桃秭林一号和清香的最佳采收期为8月27日左右。
中图分类号:
谈亚丽, 高梦祥, 李晓洁, 周英杰, 熊健, 曾子琦, 李啸, 杨华. 湖北省主栽核桃在不同采收期的品质[J]. 浙江农业学报, 2025, 37(7): 1459-1468.
TAN Yali, GAO Mengxiang, LI Xiaojie, ZHOU Yingjie, XIONG Jian, ZENG Ziqi, LI Xiao, YANG Hua. Quality of mainly cultivated walnuts in Hubei Province of China at different harvest periods[J]. Acta Agriculturae Zhejiangensis, 2025, 37(7): 1459-1468.
图1 不同采收期核桃的横径和纵径 同组柱上无相同大写字母表示同一采收期不同品种之间存在显著性差异(P<0.05);同颜色柱上无相同小写字母表示同一品种不同采收期之间存在显著性差异(P<0.05)。图2~5同。
Fig.1 Transverse and longitudinal diameters of walnuts at different harvest periods The absence of identical capital letters on the same column indicates significant differences between different varieties during the same harvesting period(P<0.05). The absence of identical lowercase letters on the same colored column indicates significant differences between different harvest periods for the same variety(P<0.05). Fig. 2-Fig. 5 are the same.
氨基酸种类 Types of amino acid | 不同采收期含量Contents at different harvest periods | |||||||
---|---|---|---|---|---|---|---|---|
秭林一号Zilin No. 1 | 清香Qingxiang | |||||||
Ⅰ | Ⅱ | Ⅲ | Ⅳ | Ⅰ | Ⅱ | Ⅲ | Ⅳ | |
天冬氨酸Aspartic acid | 34.8±0.6 a | 25.1±0.4 b | 23.1±0.3 c | 25.0±0.1 b | 24.1±0.8 a | 10.6±0.3 c | 15.1±1.0 b | 15.8±0.7 b |
苏氨酸*Threonine | 8.7±0.2 a | 7.6±0.8 ab | 6.7±0.5 b | 6.8±0.7 b | 8.0±0.8 a | 4.1±0.4 c | 5.8±1.1 b | 5.9±1.1 b |
丝氨酸Serine | 12.4±0.2 a | 11.4±1.6 a | 10.8±0.1 a | 11.1±1.1 a | 10.6±2.9 a | 6.0±0.4 b | 9.2±1.7 ab | 9.4±2.0 ab |
谷氨酸Glutamate | 67.1±0.9 a | 47.0±0.6 b | 43.6±0.3 c | 38.7±0.3 d | 40.0±0.4 a | 20.0±0.4 c | 30.4±0.3 b | 31.2±1.4 b |
甘氨酸Glycine | 12.6±0.2 a | 10.2±0.3 b | 10.3±0.5 b | 9.7±1.4 b | 9.0±2.3 a | 5.6±0.3 b | 8.9±1.0 a | 8.7±2.1 ab |
丙氨酸Alanine | 10.2±0.9 a | 10.0±0.9 a | 10.2±0.5 a | 9.6±0.9 a | 8.9±2.2 a | 5.8±0.8 a | 8.6±0.6 a | 8.4±2.2 a |
胱氨酸Cystine | 1.8±0.1 a | 1.9±0.4 a | 1.0±0.2 b | 0.7±0.3 b | 2.2±0.3 a | 1.2±0.4 b | 0.8±0.2 b | 1.1±0.8 b |
缬氨酸*Valine | 10.7±0.8a | 10.7±0.8 a | 10.2±0.3 a | 10.7±0.8 a | 10.5±2.2 a | 5.7±0.2 b | 8.6±0.6 a | 8.4±0.7 a |
异亮氨酸*Isoleucine | 8.9±0.3 a | 8.2±0.6 ab | 8.5±0.4 ab | 7.6±0.7 b | 9.3±1.7 a | 4.4±0.2 c | 6.9±0.5 b | 6.4±1.4 b |
亮氨酸*Leucine | 29.0±0.2 a | 17.1±0.6 b | 15.5±0.3 c | 15.3±0.2 c | 20.9±0.5 a | 9.6±0.2 d | 12.8±0.2 b | 10.4±0.5 c |
酪氨酸Tyrosine | 12.3±0.2 a | 2.5±1.6 d | 8.1±0.1 b | 5.3±0.4 c | 9.4±0.4 a | 5.6±0.7 b | 7.6±0.3 ab | 4.7±0.7 b |
苯丙氨酸*Phenylalanine | 10.1±0.4 a | 10.2±0.7 a | 10.2±0.3 a | 10.2±0.7 a | 10.5±1.7 b | 5.8±0.7 d | 14.0±0.9 a | 8.4±0.8 c |
赖氨酸*Lysine | 6.5±0.2 a | 6.7±0.4 a | 5.4±0.2 b | 5.9±0.4 b | 8.0±0.3 a | 3.6±0.3 d | 6.7±0.3 b | 5.3±1.0 c |
组氨酸Histidine | 6.4±0.2 a | 5.4±0.5 b | 4.3±0.2 c | 4.9±0.6 bc | 5.7±1.1 a | 2.7±0.2 c | 5.0±0.4 ab | 4.3±0.8 b |
精氨酸Arginine | 50.2±0.4 a | 29.6±0.6 c | 28.3±0.5 d | 38.7±0.4 b | 30.1±0.4 a | 16.2±0.3 d | 24.2±0.3 c | 27.1±0.9 b |
脯氨酸Proline | 10.1±0.2 b | 8.5±0.4 c | 6.8±0.1 d | 14.2±0.8 a | 7.7±0.4 b | 4.4±0.3 c | 6.8±0.7 b | 11.4±2.5 a |
表1 不同采收期核桃的氨基酸组成与含量
Table 1 Composition and contents of amino acids of walnuts at different harvest periods g·kg-1
氨基酸种类 Types of amino acid | 不同采收期含量Contents at different harvest periods | |||||||
---|---|---|---|---|---|---|---|---|
秭林一号Zilin No. 1 | 清香Qingxiang | |||||||
Ⅰ | Ⅱ | Ⅲ | Ⅳ | Ⅰ | Ⅱ | Ⅲ | Ⅳ | |
天冬氨酸Aspartic acid | 34.8±0.6 a | 25.1±0.4 b | 23.1±0.3 c | 25.0±0.1 b | 24.1±0.8 a | 10.6±0.3 c | 15.1±1.0 b | 15.8±0.7 b |
苏氨酸*Threonine | 8.7±0.2 a | 7.6±0.8 ab | 6.7±0.5 b | 6.8±0.7 b | 8.0±0.8 a | 4.1±0.4 c | 5.8±1.1 b | 5.9±1.1 b |
丝氨酸Serine | 12.4±0.2 a | 11.4±1.6 a | 10.8±0.1 a | 11.1±1.1 a | 10.6±2.9 a | 6.0±0.4 b | 9.2±1.7 ab | 9.4±2.0 ab |
谷氨酸Glutamate | 67.1±0.9 a | 47.0±0.6 b | 43.6±0.3 c | 38.7±0.3 d | 40.0±0.4 a | 20.0±0.4 c | 30.4±0.3 b | 31.2±1.4 b |
甘氨酸Glycine | 12.6±0.2 a | 10.2±0.3 b | 10.3±0.5 b | 9.7±1.4 b | 9.0±2.3 a | 5.6±0.3 b | 8.9±1.0 a | 8.7±2.1 ab |
丙氨酸Alanine | 10.2±0.9 a | 10.0±0.9 a | 10.2±0.5 a | 9.6±0.9 a | 8.9±2.2 a | 5.8±0.8 a | 8.6±0.6 a | 8.4±2.2 a |
胱氨酸Cystine | 1.8±0.1 a | 1.9±0.4 a | 1.0±0.2 b | 0.7±0.3 b | 2.2±0.3 a | 1.2±0.4 b | 0.8±0.2 b | 1.1±0.8 b |
缬氨酸*Valine | 10.7±0.8a | 10.7±0.8 a | 10.2±0.3 a | 10.7±0.8 a | 10.5±2.2 a | 5.7±0.2 b | 8.6±0.6 a | 8.4±0.7 a |
异亮氨酸*Isoleucine | 8.9±0.3 a | 8.2±0.6 ab | 8.5±0.4 ab | 7.6±0.7 b | 9.3±1.7 a | 4.4±0.2 c | 6.9±0.5 b | 6.4±1.4 b |
亮氨酸*Leucine | 29.0±0.2 a | 17.1±0.6 b | 15.5±0.3 c | 15.3±0.2 c | 20.9±0.5 a | 9.6±0.2 d | 12.8±0.2 b | 10.4±0.5 c |
酪氨酸Tyrosine | 12.3±0.2 a | 2.5±1.6 d | 8.1±0.1 b | 5.3±0.4 c | 9.4±0.4 a | 5.6±0.7 b | 7.6±0.3 ab | 4.7±0.7 b |
苯丙氨酸*Phenylalanine | 10.1±0.4 a | 10.2±0.7 a | 10.2±0.3 a | 10.2±0.7 a | 10.5±1.7 b | 5.8±0.7 d | 14.0±0.9 a | 8.4±0.8 c |
赖氨酸*Lysine | 6.5±0.2 a | 6.7±0.4 a | 5.4±0.2 b | 5.9±0.4 b | 8.0±0.3 a | 3.6±0.3 d | 6.7±0.3 b | 5.3±1.0 c |
组氨酸Histidine | 6.4±0.2 a | 5.4±0.5 b | 4.3±0.2 c | 4.9±0.6 bc | 5.7±1.1 a | 2.7±0.2 c | 5.0±0.4 ab | 4.3±0.8 b |
精氨酸Arginine | 50.2±0.4 a | 29.6±0.6 c | 28.3±0.5 d | 38.7±0.4 b | 30.1±0.4 a | 16.2±0.3 d | 24.2±0.3 c | 27.1±0.9 b |
脯氨酸Proline | 10.1±0.2 b | 8.5±0.4 c | 6.8±0.1 d | 14.2±0.8 a | 7.7±0.4 b | 4.4±0.3 c | 6.8±0.7 b | 11.4±2.5 a |
指标 | TD | LD | KY | GFD | MC | FAT | PRO | TAA | Glu | Arg | Asp | Ca |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Index | ||||||||||||
LD | 0.935** | |||||||||||
KY | 0.557** | 0.629** | ||||||||||
GFD | 0.597** | 0.651** | 0.832** | |||||||||
MC | -0.394 | -0.472* | -0.796** | -0.795** | ||||||||
FAT | 0.420* | 0.442* | 0.560** | 0.790** | -0.844** | |||||||
PRO | -0.009 | -0.099 | -0.550** | -0.526** | 0.857** | -0.721** | ||||||
TAA | 0.145 | 0.130 | -0.276 | -0.208 | 0.648** | -0.554** | 0.840** | |||||
Glu | 0.145 | 0.100 | -0.273 | -0.326 | 0.700** | -0.680** | 0.887** | 0.956** | ||||
Arg | 0.263 | 0.225 | -0.141 | -0.032 | 0.546** | -0.458* | 0.808** | 0.937** | 0.893** | |||
Asp | 0.193 | 0.149 | -0.323 | -0.271 | 0.679** | 0.557** | 0.892** | 0.970** | 0.964** | 0.925** | ||
Ca | -0.265 | -0.340 | -0.659** | -0.650** | 0.964** | -0.838** | 0.911** | 0.768** | 0.814** | 0.714** | 0.785** | |
Zn | 0.269 | 0.220 | -0.337 | -0.257 | 0.649** | -0.496* | 0.906** | 0.916** | 0.917** | 0.897** | 0.968** | 0.755** |
表2 核桃各品质指标间的相关性
Table 2 Correlation between each quality indicator of walnuts
指标 | TD | LD | KY | GFD | MC | FAT | PRO | TAA | Glu | Arg | Asp | Ca |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Index | ||||||||||||
LD | 0.935** | |||||||||||
KY | 0.557** | 0.629** | ||||||||||
GFD | 0.597** | 0.651** | 0.832** | |||||||||
MC | -0.394 | -0.472* | -0.796** | -0.795** | ||||||||
FAT | 0.420* | 0.442* | 0.560** | 0.790** | -0.844** | |||||||
PRO | -0.009 | -0.099 | -0.550** | -0.526** | 0.857** | -0.721** | ||||||
TAA | 0.145 | 0.130 | -0.276 | -0.208 | 0.648** | -0.554** | 0.840** | |||||
Glu | 0.145 | 0.100 | -0.273 | -0.326 | 0.700** | -0.680** | 0.887** | 0.956** | ||||
Arg | 0.263 | 0.225 | -0.141 | -0.032 | 0.546** | -0.458* | 0.808** | 0.937** | 0.893** | |||
Asp | 0.193 | 0.149 | -0.323 | -0.271 | 0.679** | 0.557** | 0.892** | 0.970** | 0.964** | 0.925** | ||
Ca | -0.265 | -0.340 | -0.659** | -0.650** | 0.964** | -0.838** | 0.911** | 0.768** | 0.814** | 0.714** | 0.785** | |
Zn | 0.269 | 0.220 | -0.337 | -0.257 | 0.649** | -0.496* | 0.906** | 0.916** | 0.917** | 0.897** | 0.968** | 0.755** |
变量 Variable | 主成分1 PC1 | 主成分2 PC2 |
---|---|---|
蛋白质含量Protein content | 0.98 | -0.08 |
谷氨酸含量Glutamate content | 0.96 | 0.15 |
天冬氨酸含量Aspartic acid content | 0.96 | 0.26 |
总氨基酸含量Total amino acid content | 0.95 | 0.28 |
锌含量Zinc content | 0.93 | 0.29 |
钙含量Calcium content | 0.92 | -0.34 |
精氨酸含量Arginine content | 0.89 | 0.37 |
含水率Moisture content | 0.84 | -0.50 |
脂肪含量Fat content | -0.75 | 0.58 |
特征值Eigenvalue | 7.487 | 1.096 |
方差贡献率Variance contribution rate/% | 83.184 | 12.172 |
累计贡献率Cumulative contribution rate/% | 83.184 | 95.356 |
表3 主成分方差特征值、贡献率和载荷矩阵值
Table 3 Variance eigenvalues, contribution rates, and load matrix values of principal components
变量 Variable | 主成分1 PC1 | 主成分2 PC2 |
---|---|---|
蛋白质含量Protein content | 0.98 | -0.08 |
谷氨酸含量Glutamate content | 0.96 | 0.15 |
天冬氨酸含量Aspartic acid content | 0.96 | 0.26 |
总氨基酸含量Total amino acid content | 0.95 | 0.28 |
锌含量Zinc content | 0.93 | 0.29 |
钙含量Calcium content | 0.92 | -0.34 |
精氨酸含量Arginine content | 0.89 | 0.37 |
含水率Moisture content | 0.84 | -0.50 |
脂肪含量Fat content | -0.75 | 0.58 |
特征值Eigenvalue | 7.487 | 1.096 |
方差贡献率Variance contribution rate/% | 83.184 | 12.172 |
累计贡献率Cumulative contribution rate/% | 83.184 | 95.356 |
品种 Variety | 采收期 Harvest period | X1 含水率 Moisture content | X2 脂肪含量 Fat content | X3 蛋白质含量 Protein content | X4 总氨基酸含量 Total amino acid content | X5 谷氨酸含量 Glutamate content | X6 精氨酸含量 Arginine content | X7 天冬氨酸含量 Aspartic acid content | X8 钙含量 Calcium content | X9 锌含量 Zinc content |
---|---|---|---|---|---|---|---|---|---|---|
秭林一号 | Ⅰ | -0.33 | 0.39 | -0.06 | 0.10 | 0.28 | -0.22 | 0.19 | -0.24 | 0.43 |
Zilin No. 1 | Ⅱ | 0.23 | -0.58 | 0.34 | 0.27 | 0.52 | -0.10 | 0.44 | -0.05 | 0.26 |
Ⅲ | -0.82 | 1.22 | 0.04 | 0.32 | -0.07 | 0.80 | 0.43 | -0.59 | 0.66 | |
Ⅳ | 1.78 | -2.00 | 1.97 | 1.81 | 1.96 | 1.94 | 1.73 | 2.11 | 1.67 | |
清香 | Ⅰ | -1.11 | -0.09 | -0.52 | 0.32 | 0.01 | -0.04 | 0.31 | 0.81 | 0.21 |
Qingxiang | Ⅱ | -0.10 | -0.05 | -0.69 | 1.68 | -1.41 | -1.42 | 1.46 | -0.40 | -1.19 |
Ⅲ | -0.87 | 0.10 | 1.12 | -0.52 | -0.67 | -0.63 | 0.87 | 0.83 | -1.09 | |
Ⅳ | 0.99 | 1.00 | 1.01 | 0.60 | 0.61 | 0.34 | 0.77 | -0.81 | -0.95 |
表4 各指标的标准化值
Table 4 Standardized values of each index
品种 Variety | 采收期 Harvest period | X1 含水率 Moisture content | X2 脂肪含量 Fat content | X3 蛋白质含量 Protein content | X4 总氨基酸含量 Total amino acid content | X5 谷氨酸含量 Glutamate content | X6 精氨酸含量 Arginine content | X7 天冬氨酸含量 Aspartic acid content | X8 钙含量 Calcium content | X9 锌含量 Zinc content |
---|---|---|---|---|---|---|---|---|---|---|
秭林一号 | Ⅰ | -0.33 | 0.39 | -0.06 | 0.10 | 0.28 | -0.22 | 0.19 | -0.24 | 0.43 |
Zilin No. 1 | Ⅱ | 0.23 | -0.58 | 0.34 | 0.27 | 0.52 | -0.10 | 0.44 | -0.05 | 0.26 |
Ⅲ | -0.82 | 1.22 | 0.04 | 0.32 | -0.07 | 0.80 | 0.43 | -0.59 | 0.66 | |
Ⅳ | 1.78 | -2.00 | 1.97 | 1.81 | 1.96 | 1.94 | 1.73 | 2.11 | 1.67 | |
清香 | Ⅰ | -1.11 | -0.09 | -0.52 | 0.32 | 0.01 | -0.04 | 0.31 | 0.81 | 0.21 |
Qingxiang | Ⅱ | -0.10 | -0.05 | -0.69 | 1.68 | -1.41 | -1.42 | 1.46 | -0.40 | -1.19 |
Ⅲ | -0.87 | 0.10 | 1.12 | -0.52 | -0.67 | -0.63 | 0.87 | 0.83 | -1.09 | |
Ⅳ | 0.99 | 1.00 | 1.01 | 0.60 | 0.61 | 0.34 | 0.77 | -0.81 | -0.95 |
品种 Variety | 采收期 Harvest period | F1 | F2 | F | 排名 Ranking |
---|---|---|---|---|---|
秭林一号 | Ⅰ | -0.08 | 0.66 | 0.02 | 4 |
Zilin No. 1 | Ⅱ | 0.29 | 0.56 | 0.31 | 3 |
Ⅲ | 0.28 | 0.84 | 0.34 | 2 | |
Ⅳ | 3.32 | 0.99 | 2.89 | 1 | |
清香 | Ⅰ | -0.22 | -0.11 | -0.20 | 4 |
Qingxiang | Ⅱ | 0.01 | 0.40 | 0.05 | 3 |
Ⅲ | 0.33 | -0.45 | 0.22 | 2 | |
Ⅳ | 1.86 | 0.66 | 1.63 | 1 |
表5 秭林一号和清香核桃在不同采收期下的综合得分与排序
Table 5 Comprehensive score and ranking of Zilin No.1 and Qingxiang Walnut at different harvest periods
品种 Variety | 采收期 Harvest period | F1 | F2 | F | 排名 Ranking |
---|---|---|---|---|---|
秭林一号 | Ⅰ | -0.08 | 0.66 | 0.02 | 4 |
Zilin No. 1 | Ⅱ | 0.29 | 0.56 | 0.31 | 3 |
Ⅲ | 0.28 | 0.84 | 0.34 | 2 | |
Ⅳ | 3.32 | 0.99 | 2.89 | 1 | |
清香 | Ⅰ | -0.22 | -0.11 | -0.20 | 4 |
Qingxiang | Ⅱ | 0.01 | 0.40 | 0.05 | 3 |
Ⅲ | 0.33 | -0.45 | 0.22 | 2 | |
Ⅳ | 1.86 | 0.66 | 1.63 | 1 |
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