植物肌醇半乳糖苷合成酶家族基因功能的研究进展

doi:10.3969/j.issn.1004-1524.20240115

浙江农业学报 ›› 2025, Vol. 37 ›› Issue (8): 1817-1824.DOI: 10.3969/j.issn.1004-1524.20240115

• 综述 • 上一篇

植物肌醇半乳糖苷合成酶家族基因功能的研究进展

刘岩(), 林天宝, 吕志强()

浙江省农业科学院蚕桑与茶叶研究所,浙江杭州 310021

收稿日期:2024-01-30 出版日期:2025-08-25 发布日期:2025-09-03
作者简介:刘岩(1976—),女,河南焦作人,博士,副研究员,主要从事桑树遗传育种研究。E-mail:mayanly@sina.com
通讯作者: *吕志强,E-mail:13958131715@139.com
基金资助:
中国-乌兹别克斯坦国家重点研发国际合作项目(2022YFE012700);“十四五”农业新品种选育重大育种专项蚕桑协作项目(2021C02072);财政部和农业农村部国家现代农业产业技术体系项目;浙江省农业科学院科技新成果示范推广项目(tg2022015)

Research progress on the function of galactinol synthase gene family in plants

LIU Yan(), LIN Tianbao, LYU Zhiqiang()

Institute of Sericulture and Tea Research, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2024-01-30 Online:2025-08-25 Published:2025-09-03
Contact: LYU Zhiqiang

摘要/Abstract

摘要：

棉子糖家族寡糖(RFOs)是植物重要碳水化合物贮运成分,肌醇半乳糖苷合成酶(GolS)作为RFOs生物合成的关键限速酶,参与影响碳储存、韧皮部运输、渗透调节及逆境信号传导,在生长发育和逆境胁迫响应中也发挥重要作用。本文结合近些年国内外关于植物肌醇半乳糖苷合成酶基因家族特性、生物学功能等研究近况,阐述GolS家族基因在影响植物同化物运输、种子发育、生物及非生物胁迫响应中的作用,分析未来可进一步探究GolS基因的表达调控机制,挖掘其在作物品种改良和抗逆育种中的应用潜力,为农业生产提供理论支持和技术参考。

关键词: 棉子糖家族寡糖(RFOs), GolS基因, 胁迫耐受

Abstract:

Raffinose family oligosaccharides (RFOs) are widespread carbohydrates functioning in storage and transport in plants. Galactinol synthase (GolS), a key rate-limiting enzyme in raffinose biosynthesis, is involved in regulating carbon storage, phloem transport, osmotic adjustment, and stress signal transduction, and also plays a crucial role in plant growth, development, and stress responses. In the present paper, we review the research progress on the structural characteristics and biological functions of plant GolS gene family, and summarize its roles in plant assimilate transport, seed development, and cell secondary wall formation, as well as its ability to enhance plant stress resistance by regulating cellular osmotic pressure and antioxidant capacity. Future research is prospected to focus on exploring the regulatory mechanisms underlying GolS gene expression and their potential in crop improvement and stress-resistant breeding, which may provide theoretical support and technical references for agricultural production.

Key words: raffinose family oligosaccharides (RFOs), galactinol synthase, stress tolerance

中图分类号:

Q945

刘岩, 林天宝, 吕志强. 植物肌醇半乳糖苷合成酶家族基因功能的研究进展[J]. 浙江农业学报, 2025, 37(8): 1817-1824.

LIU Yan, LIN Tianbao, LYU Zhiqiang. Research progress on the function of galactinol synthase gene family in plants[J]. Acta Agriculturae Zhejiangensis, 2025, 37(8): 1817-1824.

参考文献 59

[1]	PETERBAUER T, RICHTER A. Biochemistry and physiology of raffinose family oligosaccharides and galactosyl cyclitols in seeds[J]. Seed Science Research, 2001, 11: 185-197.
[2]	CASTILLO E M, DE LUMEN B O, REYES P S, et al. Raffinose synthase and galactinol synthase in developing seeds and leaves of legumes[J]. Journal of Agricultural and Food Chemistry, 1990, 38(2): 351-355.
[3]	AYRE B G, KELLER F, TURGEON R. Symplastic continuity between companion cells and the translocation stream: long-distance transport is controlled by retention and retrieval mechanisms in the phloem[J]. Plant Physiology, 2003, 131(4): 1518-1528.
[4]	SHEEN J, ZHOU L, JANG J C. Sugars as signaling molecules[J]. Current Opinion in Plant Biology, 1999, 2(5): 410-418.
[5]	SENGUPTA S, MUKHERJEE S, BASAK P, et al. Significance of galactinol and raffinose family oligosaccharide synthesis in plants[J]. Frontiers in Plant Science, 2015, 6: 656.
[6]	张古文, 胡齐赞, 徐盛春, 等. 菜用大豆籽粒发育过程中蔗糖积累及相关酶活性的研究[J]. 浙江农业学报, 2012, 24(6): 1015-1020.
	ZHANG G W, HU Q Z, XU S C, et al. Study on sucrose accumulation and enzyme activities involved in sucrose metabolism in developing seeds of vegetable soybean[J]. Acta Agriculturae Zhejiangensis, 2012, 24(6): 1015-1020. (in Chinese with English abstract)
[7]	SENGUPTA S, MUKHERJEE S, PARWEEN S, et al. Galactinol synthase across evolutionary diverse taxa: Functional preference for higher plants?[J]. FEBS Letters, 2012, 586(10): 1488-1496.
[8]	YIN Y B, CHEN H L, HAHN M G, et al. Evolution and function of the plant cell wall synthesis-related glycosyltransferase family 8[J]. Plant Physiology, 2010, 153(4): 1729-1746.
[9]	DAI H, ZHU Z, WANG Z, et al. Galactinol synthase 1 improves cucumber performance under cold stress by enhancing assimilate translocation[J]. Horticulture Research, 2022, 9: uhab063.
[10]	TAJI T, OHSUMI C, IUCHI S, et al. Important roles of drought- and cold-inducible genes for galactinol synthase in stress tolerance in Arabidopsis thaliana[J]. The Plant Journal, 2002, 29(4): 417-426.
[11]	YOU J, WANG Y Y, ZHANG Y J, et al. Genome-wide identification and expression analyses of genes involved in raffinose accumulation in sesame[J]. Scientific Reports, 2018, 8(1): 4331.
[12]	FALAVIGNA V D S, PORTO D D, MIOTTO Y E, et al. Evolutionary diversification of galactinol synthases in Rosaceae: adaptive roles of galactinol and raffinose during apple bud dormancy[J]. Journal of Experimental Botany, 2018, 69(5): 1247-1259.
[13]	FAN Y H, YU M N, LIU M, et al. Genome-wide identification, evolutionary and expression analyses of the GALACTINOL SYNTHASE gene family in rapeseed and tobacco[J]. International Journal of Molecular Sciences, 2017, 18(12): 2768.
[14]	ZHOU J, YANG Y, YU J, et al. Responses of Populus trichocarpa galactinol synthase genes to abiotic stresses[J]. Journal of Plant Research, 2014, 127(2): 347-358.
[15]	JING Q K, CHEN A R, LV Z Y, et al. Systematic analysis of galactinol synthase and raffinose synthase gene families in potato and their expression patterns in development and abiotic stress responses[J]. Genes, 2023, 14(7): 1344.
[16]	ZHOU Y, LIU Y, WANG S S, et al. Molecular cloning and characterization of galactinol synthases in Camellia sinensis with different responses to biotic and abiotic stressors[J]. Journal of Agricultural and Food Chemistry, 2017, 65(13): 2751-2759.
[17]	HANNAH M A, ZUTHER E, BUCHEL K, et al. Transport and metabolism of raffinose family oligosaccharides in transgenic potato[J]. Journal of Experimental Botany, 2006, 57(14): 3801-3811.
[18]	MCCASKILL A, TURGEON R. Phloem loading in Verbascum phoeniceum L. depends on the synthesis of raffinose-family oligosaccharides[J]. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104(49): 19619-19624.
[19]	MA S, SUN L L, SUI X L, et al. Phloem loading in cucumber: combined symplastic and apoplastic strategies[J]. The Plant Journal, 2019, 98(3): 391-404.
[20]	韦玉霞. 园艺作物中棉子糖系列寡糖(RFO)研究进展[J]. 园艺与种苗, 2017, 37(9): 46-48.
	WEI Y X. Research progress of raffinose family oligosaccharides(RFO) in horticultural crops[J]. Horticulture & Seed, 2017, 37(9): 46-48. (in Chinese with English abstract)
[21]	张瑞腾, 吕建春, 周梦迪, 等. 甜瓜肌醇半乳糖苷合成酶基因CmGAS1的表达与功能分析[J]. 园艺学报, 2018, 45(10): 1929-1940.
	ZHANG R T, LÜ J C, ZHOU M D, et al. Expression and function analysis of CmGAS1 in melon[J]. Acta Horticulturae Sinica, 2018, 45(10): 1929-1940. (in Chinese with English abstract)
[22]	JING Y, LANG S R, WANG D M, et al. Functional characterization of galactinol synthase and raffinose synthase in desiccation tolerance acquisition in developing Arabidopsis seeds[J]. Journal of Plant Physiology, 2018, 230: 109-121.
[23]	LI X, ZHUO J J, JING Y, et al. Expression of a GALACTINOL SYNTHASE gene is positively associated with desiccation tolerance of Brassica napus seeds during development[J]. Journal of Plant Physiology, 2011, 168(15): 1761-1770.
[24]	SALVI P, SAXENA S C, PETLA B P, et al. Differentially expressed galactinol synthase(s) in chickpea are implicated in seed vigor and longevity by limiting the age induced ROS accumulation[J]. Scientific Reports, 2016, 6: 35088.
[25]	LE H, NGUYEN N H, TA D T, et al. CRISPR/Cas9-mediated knockout of galactinol synthase-encoding genes reduces raffinose family oligosaccharide levels in soybean seeds[J]. Frontiers in Plant Science, 2020, 11: 612942.
[26]	GANGOLA M P, JAISWAL S, KANNAN U, et al. Galactinol synthase enzyme activity influences raffinose family oligosaccharides (RFO) accumulation in developing chickpea (Cicer arietinum L.) seeds[J]. Phytochemistry, 2016, 125: 88-98.
[27]	JANG J H, SHANG Y, KANG H K, et al. Arabidopsis galactinol synthases 1 (AtGOLS1) negatively regulates seed germination[J]. Plant Science, 2018, 267: 94-101.
[28]	UNDA F, KIM H, HEFER C, et al. Altering carbon allocation in hybrid poplar (Populus alba × grandidentata) impacts cell wall growth and development[J]. Plant Biotechnology Journal, 2017, 15(7): 865-878.
[29]	范洁. 木薯肌醇半乳糖苷合成酶基因MeGolS5的抗旱功能研究[D]. 海口: 海南大学, 2015.
	FAN J. Study on drought resistance of cassava inositol galactoside synthase gene MeGolS5[D]. Haikou: Hainan University, 2015. (in Chinese with English abstract)
[30]	张军, 邱爽, 何佳琦, 等. 大豆GmGolS基因植物表达载体构建及烟草遗传转化[J]. 齐齐哈尔大学学报(自然科学版), 2020, 36(6): 22-25.
	ZHANG J, QIU S, HE J Q, et al. Plant expression vector construction and tobacco genetic transformation of soybean GmGolS gene[J]. Journal of Qiqihar University(Natural Science Edition), 2020, 36(6): 22-25. (in Chinese with English abstract)
[31]	刘爱丽, 魏梦园, 黎冬华, 等. 芝麻肌醇半乳糖苷合成酶基因SiGolS6的克隆及功能分析[J]. 中国农业科学, 2020, 53(17): 3432-3442.
	LIU A L, WEI M Y, LI D H, et al. Cloning and function analysis of sesame galactinol synthase gene SiGolS6 in Arabidopsis[J]. Scientia Agricultura Sinica, 2020, 53(17): 3432-3442. (in Chinese with English abstract)
[32]	SHIKAKURA Y, OGUCHI T, YU X, et al. Transgenic poplar trees overexpressing AtGolS2, a stress-responsive galactinol synthase gene derived from Arabidopsis thaliana, improved drought tolerance in a confined field[J]. Transgenic Research, 2022, 31(4): 579-591.
[33]	SELVARAJ M G, ISHIZAKI T, VALENCIA M, et al. Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field[J]. Plant Biotechnology Journal, 2017, 15(11): 1465-1477.
[34]	LIU Y D, ZHANG L, CHEN L J, et al. Molecular cloning and expression of an encoding galactinol synthase gene (AnGolS1) in seedling of Ammopiptanthus nanus[J]. Scientific Reports, 2016, 6: 36113.
[35]	孙海伟. 沙冬青肌醇半乳糖苷合成酶(AmGS)基因转化类茶植物红叶石楠的研究[D]. 泰安: 山东农业大学, 2012.
	SUN H W. Study on transformation of ammopiptanthus mongolicus galactosidase (AmGS) gene into tea-like plant Photinia rubra[D]. Taian: Shandong Agricultural University, 2012. (in Chinese with English abstract)
[36]	SONG J, LIU J, WENG M L, et al. Cloning of galactinol synthase gene from Ammopiptanthus mongolicus and its expression in transgenic Photinia serrulata plants[J]. Gene, 2013, 513(1): 118-127.
[37]	王海波, 邹竹荣, 龚明. 小桐子肌醇半乳糖苷合成酶3的基因克隆及其生物信息学分析和功能初步验证[J]. 生物技术通报, 2015, 31(7): 91-100.
	WANG H B, ZOU Z R, GONG M. Gene cloning, bioinformatic analysis and preliminary functional characterization of gene encoding galactinol synthase 3 in Jatropha curcas[J]. Biotechnology Bulletin, 2015, 31(7): 91-100. (in Chinese with English abstract)
[38]	贺飞燕, 徐建飞, 简银巧, 等. 过表达肌醇半乳糖苷合成酶基因(ScGolS1)提高转基因马铃薯的耐寒性[C]// 马铃薯产业与种业创新(2022)专题资料汇编, 2022:261-262.
[39]	SHIMOSAKA E, OZAWA K. Overexpression of cold-inducible wheat galactinol synthase confers tolerance to chilling stress in transgenic rice[J]. Breeding Science, 2015, 65(5): 363-371.
[40]	ZUTHER E, BÜCHEL K, HUNDERTMARK M, et al. The role of raffinose in the cold acclimation response of Arabidopsis thaliana[J]. FEBS Letters, 2004, 576(1/2): 169-173.
[41]	赵小萌. 棉子糖寡糖提高番茄幼苗高温抗性的作用研究[D]. 沈阳: 沈阳农业大学, 2019.
	ZHAO X M. Effect of raffinose oligosaccharide on improving high temperature resistance of tomato seedlings[D]. Shenyang: Shenyang Agricultural University, 2019. (in Chinese with English abstract)
[42]	靳娟, 刘晶, 杨磊, 等. 酸枣肌醇半乳糖苷合成酶ZjGolS2基因的克隆及表达分析[J]. 分子植物育种, 2021, 19(13): 4327-4333.
	JIN J, LIU J, YANG L, et al. Cloning and expression analysis of ZjGolS2 in wild jujube(Ziziphus jujuba Mill.var.Spinosa)[J]. Molecular Plant Breeding, 2021, 19(13): 4327-4333. (in Chinese with English abstract)
[43]	沈阳, 贾博为, 王金玉, 等. 拟南芥肌醇半乳糖苷酶AtGolS2基因在非生物胁迫应答中的功能分析[J]. 分子植物育种, 2021, 19(11): 3588-3597.
	SHEN Y, JIA B W, WANG J Y, et al. Functional analysis of Arabidopsis thaliana galactinol synthase AtGolS2 in response to abiotic stress[J]. Molecular Plant Breeding, 2021, 19(11): 3588-3597. (in Chinese with English abstract)
[44]	SUN Z B, QI X Y, WANG Z L, et al. Overexpression of TsGOLS2, a galactinol synthase, in Arabidopsis thaliana enhances tolerance to high salinity and osmotic stresses[J]. Plant Physiology and Biochemistry, 2013, 69: 82-89.
[45]	KONG W L, GONG Z Y, ZHONG H, et al. Expansion and evolutionary patterns of glycosyltransferase family 8 in Gramineae crop genomes and their expression under salt and cold stresses in Oryza sativa ssp. japonica[J]. Biomolecules, 2019, 9(5): 188.
[46]	LI N, ZHANG Z H, CHEN Z J, et al. Comparative transcriptome analysis of two contrasting Chinese cabbage (Brassica rapa L.) genotypes reveals that ion homeostasis is a crucial biological pathway involved in the rapid adaptive response to salt stress[J]. Frontiers in Plant Science, 2021, 12: 683891.
[47]	MARTINS C P S, FERNANDES D, GUIMARÃES V M, et al. Comprehensive analysis of the GALACTINOL SYNTHASE (GolS) gene family in citrus and the function of CsGolS 6 in stress tolerance[J]. PLoS One, 2022, 17(9): e0274791.
[48]	WANG Y G, LIU H H, WANG S P, et al. Overexpression of a common wheat gene GALACTINOL SYNTHASE3 enhances tolerance to zinc in Arabidopsis and rice through the modulation of reactive oxygen species production[J]. Plant Molecular Biology Reporter, 2016, 34(4): 794-806.
[49]	SALVI P, KAMBLE N U, MAJEE M. Stress-inducible galactinol synthase of chickpea (CaGolS) is implicated in heat and oxidative stress tolerance through reducing stress-induced excessive reactive oxygen species accumulation free[J]. Plant and Cell Physiology, 2018, 59(1): 155-166.
[50]	VINSON C C, MOTA A P Z, PORTO B N, et al. Characterization of raffinose metabolism genes uncovers a wild Arachis galactinol synthase conferring tolerance to abiotic stresses[J]. Scientific Reports, 2020, 10(1): 15258.
[51]	KIM M S, CHO S M, KANG E Y, et al. Galactinol is a signaling component of the induced systemic resistance caused by Pseudomonas chlororaphis O6 root colonization[J]. Molecular Plant-Microbe Interactions, 2008, 21(12): 1643-1653.
[52]	WANG D H, LIU Z X, QIN Y, et al. Mulberry MnGolS2 mediates resistance to Botrytis cinerea on transgenic plants[J]. Genes, 2023, 14(10): 1912.
[53]	PHILIPPE R N, RALPH S G, MANSFIELD S D, et al. Transcriptome profiles of hybrid poplar (Populus trichocarpa×deltoides) reveal rapid changes in undamaged, systemic sink leaves after simulated feeding by forest tent caterpillar (Malacosoma disstria)[J]. New Phytologist, 2010, 188(3): 787-802.
[54]	CAO T, LAHIRI I, SINGH V, et al. Metabolic engineering of raffinose-family oligosaccharides in the phloem reveals alterations in carbon partitioning and enhances resistance to green peach aphid[J]. Frontiers in Plant Science, 2013, 4: 263.
[55]	TAKANASHI K, SHITAN N, SUGIYAMA A, et al. Galactinol synthase gene of Coptis japonica is involved in berberine tolerance[J]. Bioscience, Biotechnology, and Biochemistry , 2008, 72(2): 398-405.
[56]	SPRENGER N, KELLER F. Allocation of raffinose family oligosaccharides to transport and storage pools in Ajuga reptans: the roles of two distinct galactinol synthases[J]. The Plant Journal, 2000, 21(3): 249-258.
[57]	HINCHA D K, ZUTHER E, HEYER A G. The preservation of liposomes by raffinose family oligosaccharides during drying is mediated by effects on fusion and lipid phase transitions[J]. Biochimica et Biophysica Acta, 2003, 1612(2): 172-177.
[58]	PANIKULANGARA T J, EGGERS-SCHUMACHER G, WUNDERLICH M, et al. Galactinol synthase1. A novel heat shock factor target gene responsible for heat-induced synthesis of raffinose family oligosaccharides in Arabidopsis[J]. Plant Physiology, 2004, 136(2): 3148-3158.
[59]	WANG Z, ZHU Y, WANG L L, et al. A WRKY transcription factor participates in dehydration tolerance in Boea hygrometrica by binding to the W-box elements of the galactinol synthase (BhGolS1) promoter[J]. Planta, 2009, 230(6): 1155-1166.

植物肌醇半乳糖苷合成酶家族基因功能的研究进展

Research progress on the function of galactinol synthase gene family in plants

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献 59

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王忠, 杨洪兵, 杨帆, 陈亦凡, 侯晓敏. 细胞膜透性Δ电导率量化检测法研究初探[J]. 浙江农业学报, 2025, 37(6): 1344-1352.
[2]	曾洪学, 屈兴红. 低温胁迫对3份葛种质萌发过程中脯氨酸和抗坏血酸-谷胱甘肽循环代谢的影响[J]. 浙江农业学报, 2024, 36(7): 1558-1568.
[3]	牛钰, 李晶, 王俊文, 李瑞瑞, 田强, 武玥, 郁继华. 高等植物花青素生物合成、调控、生物活性及其检测的研究进展[J]. 浙江农业学报, 2024, 36(4): 978-996.
[4]	田晓明, 向光锋, 牟村, 吕浩, 马涛, 朱路, 彭静, 张敏, 何艳. 四种红豆属植物耐旱性综合评价[J]. 浙江农业学报, 2024, 36(2): 308-324.
[5]	莘晓月, 刘鹏. 激素调控种子休眠与萌发分子机制研究进展[J]. 浙江农业学报, 2023, 35(6): 1485-1496.
[6]	冯金林, 席晓宇, 赵世凤. 拟南芥N末端乙酰转移酶Naa50参与调控根细胞有丝分裂[J]. 浙江农业学报, 2022, 34(12): 2603-2609.
[7]	朱森林, 梅忠, 邢承华. 缺磷抑制拟南芥对镉的吸收[J]. 浙江农业学报, 2020, 32(5): 804-809.
[8]	郭妮, 刘亚敏, 周文颖, 刘玉民, 张盛楠. 外源草酸缓解马尾松根系铝毒[J]. 浙江农业学报, 2019, 31(7): 1086-1095.
[9]	李明雨, 王焱, 梁丹妮, 姚亚妮, 兰剑. 22份苜蓿种质萌发期耐盐性综合评价[J]. 浙江农业学报, 2019, 31(5): 746-755.
[10]	叶子飘, 尹建华, 陈先茂, 安婷, 段世华. 几个杂交水稻品种蜡熟期剑叶光合特性研究[J]. 浙江农业学报, 2019, 31(3): 355-364.
[11]	林义成, 傅庆林, 郭彬, 刘琛, 丁能飞. 盐胁迫对红叶石楠花青素含量及抗氧化系统的影响[J]. 浙江农业学报, 2018, 30(6): 970-977.
[12]	何雄, 肖尚月, 金图南, 罗海波, 孙金才. 鲜切竹笋伤害信号的初步研究[J]. 浙江农业学报, 2018, 30(6): 992-998.
[13]	张趁华, 郑红英, 严丹侃, 韩科雷, 彭杰军, 鲁宇文, 林林, 章东方, 陈剑平, 燕飞. 侵染蚕豆ClYVV的鉴定及其衍生的小干扰RNA的深度测序鉴定研究[J]. 浙江农业学报, 2018, 30(3): 406-412.
[14]	董静, 邢锦城, 洪立洲, 王茂文, 朱小梅, 刘冲, 温祝桂, 赵宝泉, 丁海荣. NaCl胁迫对马齿苋幼苗生长及体内离子分布的影响[J]. 浙江农业学报, 2017, 29(2): 236-243.
[15]	李冬月, 原文霞, 郑超, 王栩鸣, 周洁, 严成其, 陈剑平. bZIP转录因子在植物激素介导的抗病抗逆途径中的作用[J]. 浙江农业学报, 2017, 29(1): 168-175.