›› 2010, Vol. 22 ›› Issue (5): 596-598.

• 论文 • 上一篇    下一篇

一株韩国新型DHV的分离及RT-PCR鉴定

马秀丽1,赵立娜2,夏雪梅3,吴静1,林树乾1,姜亦飞1
  

  1. 1山东省农业科学院 家禽研究所,山东省家禽疫病诊断与免疫重点实验室,山东 济南250023;2山东农业大学 动物科技学院,山东 泰安 271018;3凯里学院 环境与生命科学学院,贵州 凯里 556011
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-09-25 发布日期:2010-09-25

Isolation and RT-PCR identification of a new-type Duck hepatitis virus

MA Xiu-li;ZHAO Li-na;XIA Xue-mei;WU Jing;LIN Shu-qian;JIANG Yi-fei   

  1. 1Institute of Poultry Science, Shandong Academy of Agricultural Sciences, Jinan 250023, China;2Shandong Agricultural University, Tai'an 271018, China; 3Department of Environment and Life Sciences, Kaili College, Kaili 556011, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-09-25 Published:2010-09-25

摘要: 从山东省某疑似鸭肝炎发病区分离到1株病毒JFX08,血清中和试验结果表明该分离毒与传统的Ⅰ型鸭肝炎病毒无交叉保护,分离毒回归3日龄雏鸭,可复制出鸭肝炎的典型症状和病理变化。根据已公布的韩国新型(基因C型)DHV的序列设计合成一对引物,进行RT-PCR扩增,得到大小约414 bp的目的条带;测序分析发现,分离毒与传统Ⅰ型DHV之间的相似性较低,而与韩国新型DHV之间的相似性高达93.2%~94.0%;进化分析结果表明,该分离毒与韩国新型DHV的遗传距离最近,属基因C型DHV。

关键词: 新型DHV, 分离, RT-PCR, 鉴定

Abstract: One stain of Duck hepatitis virus was isolated from Shandong Province and named as JFX08. The results of serum neutralization test showed that there was no cross-protection occurred in strain JFX08 and Duck hepatitis virus typeⅠ(DHV-Ⅰ). Clinical symptom and pathological lesion could be reproduced in 3-day ducklings challenged by the isolated strain. A pair of primers was designed and synthesized according to the sequence of DHV and a reverse transcriptase polymerase chain reaction method was developed for strain JFX08. The identity analysis of nucleotide sequence between strain JFX08 and N-DHV from Korean (genotype C) showed the similarity was 93.2%-94.0%. However, the nucleotide sequence identity between strain JFX08 and DHV-Ⅰ was much lower. Phylogenetic and evolutionary analysis showed that the genetic distance between the isolated strain JFX08 and N-DHV from Korean was the nearest, indicating JFX08 belonged to genotype C, which was a different branch from genotype A and B.

Key words: DHV new\|type, isolation, RT-PCR, identification