小肽对凡纳滨对虾幼体生长性能及非特异性免疫能力的影响

›› 2010, Vol. 22 ›› Issue (5): 590-595.

小肽对凡纳滨对虾幼体生长性能及非特异性免疫能力的影响

林启存^1,2,方长富^3,*,钟国防⁴,冯炎⁴,朱碧英³

¹宁波大学生命科学与生物工程学院,教育部应用海洋生物技术重点实验室，浙江宁波315211；²杭州市农业科学研究院水产研究所,浙江杭州 310024；³国家海洋局第二海洋研究所,浙江杭州 310014；⁴上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海 201306

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-09-25 发布日期:2010-09-25

Effect of dietary small peptides on growth and non-specific immunity of Penaeus vannamei larvae

LIN Qi-cun;FANG Chang-fu;*;ZHONG Guo-fang;FENG Yan;ZHU Bi-ying

¹Key Laboratory of Applied Technology of Marine Biology, Ministry of Education, College of Life Science and Biotechnology, Ningbo University, Ningbo 315211,China；²Fisheries Research Institute, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China；³Second Institute of Oceanography, Hangzhou 310012, China；⁴Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education,Shanghai Ocean University, Shanghai 201306, China

Received:1900-01-01 Revised:1900-01-01 Online:2010-09-25 Published:2010-09-25

摘要/Abstract

摘要： 在基础饲料中分别添加0(对照组)，1%，2%，3%小肽,研究小肽对凡纳滨对虾(Penaeus vannamei)幼体生长和非特异性免疫力的影响。60 d后测定凡纳滨对虾的生长性能及血清抗菌活力、溶菌酶和酚氧化酶活性,并用哈维氏弧菌进行攻毒试验。结果表明,小肽能显著提高凡纳滨对虾的生长性能(P＜0.05),1%,2%和3%处理组体长增长率分别比对照组增加了17.74%,27.75%和17.46%,体重增长率分别比对照组增加了12.15%,31.16%和19.01%；饲料系数分别比对照组下降了21.57%,33.99%和25.49%；成活率分别比对照组提高了12.23%,18.89%和8.89%。小肽能显著增强凡纳滨对虾的非特异性免疫能力,1%,2%,3%处理组抗菌活力分别比对照组提高了21.05%,42.10%,10.53%；血清溶菌酶比活力分别比对照组提高了16.54%,24.56%,15.54%；酚氧化酶活力分别比对照组提高了25.70%,41.90%,24.02%。哈维氏弧菌攻毒试验结果表明,5 ｄ内对照组累计死亡率为96.7%,高于各处理组,但差异不显著；综合试验结果得知,饲料中添加小肽能显著提高凡纳滨对虾生长性能和血清非特异性免疫能力,2%为适宜添加量。

关键词: 小肽, 凡纳滨对虾, 幼体, 生长, 非特异性免疫

Abstract: The present study was conducted to determine the effect of small peptides on growth performance and non-specific immune of Penaeus vannamei. Four isonitrogenous and isoenergetic experimental diets were formulated. Diet containing no small peptides served as the control diet. The other three trial diets was added 1%, 2%, 3% small peptides respectively. After 60 d-feeding, growth rate, serum antibacterial activity, lysozyme activity and phenol oxidase(PO) activity were measured, a challenge test was conducted with Vibrio harveyi. The results showed that there was significant difference in length gain, weight gain, feed coefficient and survival rate (P<0.05) of Penaeus vannamei among the control group and the treatments. The length gain of 1%, 2% and 3% trial group was 17.74%, 27.75% and 17.46% higher than that of the control, respectively. The weight gain of shrimp was 12.15%, 31.16% and 19.01% higher than that of the control, respectively. The feed coefficient was 21.57%, 33.99% and 25.49% lower than that of the control, respectively. The survival rate was 12.23%, 18.89% and 8.89% higher than that of the control, respectively. Small peptides significantly enhanced(P<0.05)non-specific immune activity of Penaeus vannamei. The antibacterial activity of 1%, 2% and 3% trial group were 21.05%, 42.10% and 10.53% higher than that of the control, respectively. The lysozyme activity were 16.54%, 24.56% and 15.54%, PO activity were 25.70%, 41.90% and 24.02% higher than that of the control, respectively. The results of Vibrio harveyi challenge showed that cumulative mortality of the control in the five days was 96.7% and higher than that of the trial groups. It was concluded that the upper limit of small peptides for optimal growth performance and non-specific immune function in Penaeus vannamei feed was about 2%．

Key words: small peptides, Penaeus vannamei, larvae, growth, non-specific immunity

林启存;方长富;*;钟国防;冯炎;朱碧英. 小肽对凡纳滨对虾幼体生长性能及非特异性免疫能力的影响[J]. , 2010, 22(5): 590-595.

LIN Qi-cun;FANG Chang-fu;*;ZHONG Guo-fang;FENG Yan;ZHU Bi-ying. Effect of dietary small peptides on growth and non-specific immunity of Penaeus vannamei larvae[J]. , 2010, 22(5): 590-595.

[1]	王铁, 黄胜佳, 杨友婷, 谭丽平, 邱霞, 董甜甜, 黎思辰, 孙国超, 熊博, 王均, 汪志辉. 不同中间砧对媛小春柑橘生长与光合特性的影响[J]. 浙江农业学报, 2021, 33(3): 413-421.
[2]	葛金涛, 王江英, 赵文静, 邵小斌, 朱朋波, 汤雪燕, 孙明伟, 刘兴满. 魏可葡萄气生根发育的转录组分析[J]. 浙江农业学报, 2020, 32(9): 1645-1655.
[3]	陈天, 包宁颖, 杜崇宣, 刘云根. 不同砷污染程度下香蒲生长与砷富集特征[J]. 浙江农业学报, 2020, 32(9): 1672-1682.
[4]	许娜, 王大海, 杜传印, 杜沙沙, 王晓萌, 张彦, 张玉琴, 吴元华, 管恩森, 石屹. 株距对烟苗生长发育的影响[J]. 浙江农业学报, 2020, 32(8): 1342-1350.
[5]	兰挚谦, 张凯歌, 张雪艳. 耕层厚度对黄瓜叶片光合荧光与根系生理特性的影响[J]. 浙江农业学报, 2020, 32(7): 1196-1205.
[6]	刘连忠, 李孟杰, 宁井铭. 基于时序巡航图像的茶树生长监测研究[J]. 浙江农业学报, 2020, 32(5): 886-896.
[7]	白俊艳, 卢军浩, 付学言, 武晓红, 杨又兵, 雷莹, 庞有志, 卢小宁, 巩慧荣, 胡陆星, 刘红涛, 樊红灯, 曹恒, 时坤鹏, 陈梦柯, 马永康. 蛋用鹌鹑IGF-1R基因的多态性与体尺性状相关性分析[J]. 浙江农业学报, 2020, 32(3): 398-405.
[8]	黄科文, 李克强, 刘继, 岁立云, 刘磊, 王铤, 郑阳霞, 林立金, 廖明安. 不同砧穗组合对豆瓣菜扦插苗生理特性及硒积累的影响[J]. 浙江农业学报, 2020, 32(3): 447-454.
[9]	耿艳飞, 吕明芳. 植物富含半胱氨酸类受体激酶家族研究进展[J]. 浙江农业学报, 2020, 32(12): 2303-2312.
[10]	王塑天, 孟繁明, 胡斌, 辛海云, 李宝红, 杜宗亮, 李剑豪. 藏猪在亚热带条件下的生长特性及其杂交利用效果[J]. 浙江农业学报, 2020, 32(11): 1963-1969.
[11]	马瑞芳, 马进川, 马军伟, 周大云, 王强, 丁潮洪. 四季豆丽芸2号生长动态及其养分吸收规律[J]. 浙江农业学报, 2020, 32(1): 80-85.
[12]	刘加林, 刘士力, 蒋文枰, 程顺, 迟美丽, 郑建波, 贾永义, 赵金良, 尹绍武, 顾志敏. 河川沙塘鳢GH基因及侧翼的克隆与生物信息学分析[J]. 浙江农业学报, 2019, 31(9): 1461-1470.
[13]	刘慧洁, 徐恒, 邱文怡, 李晓芳, 张华, 朱英, 李春寿, 王良超. 转录因子在植物生长发育及非生物逆境响应的作用[J]. 浙江农业学报, 2019, 31(7): 1205-1214.
[14]	李刚波, 张梅, 樊继德, 赵林, 张婷, 蔺经, 常有宏, 杨峰. 徐淮地区避雨栽培对早熟砂梨果实糖积累和营养生长的影响[J]. 浙江农业学报, 2019, 31(6): 900-907.
[15]	黄展锐, 王雅玲, 杜映慧, 王小博, 孙力军, 莫冰, 毕思远. 生物统计法分析T-2毒素对凡纳滨对虾形态指标的影响[J]. 浙江农业学报, 2019, 31(3): 372-378.