壳寡糖-纳米银的抗菌活性及其对红花种子萌发和幼苗生长的影响

doi:10.3969/j.issn.1004-1524.20250117

浙江农业学报 ›› 2026, Vol. 38 ›› Issue (1): 76-84.DOI: 10.3969/j.issn.1004-1524.20250117

壳寡糖-纳米银的抗菌活性及其对红花种子萌发和幼苗生长的影响

原增艳¹(), 任欣欣², 宋小锋², 朱畇昊³^,^*()

1.豫北医学院医学检验学院,河南新乡 453003
2.豫北医学院生命科学技术学院,河南新乡 453003
3.河南中医药大学药学院,河南郑州 450046

收稿日期:2025-02-17 出版日期:2026-01-25 发布日期:2026-02-11
作者简介:*朱畇昊,E-mail:guxinhan123@163.com
原增艳,主要从事生物制药方向的研究。E-mail:yzyxx2008@126.com
通讯作者: 朱畇昊
基金资助:
民办普通高等学校学科建设项目(zdxk2021001);河南省重点学科(教研〔2023〕414号—1058)

Antifungal activity of chitooligosaccharide-nano silver and its effects on safflower seed germination and seedling growth

YUAN Zengyan¹(), REN Xinxin², SONG Xiaofeng², ZHU Yunhao³^,^*()

1. School of Medical Laboratory, North Henan Medical University, Xinxiang 453003, Henan, China
2. School of Life Science and Biotechnology, North Henan Medical University, Xinxiang 453003, Henan, China
3. School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China

Received:2025-02-17 Online:2026-01-25 Published:2026-02-11
Contact: ZHU Yunhao

摘要/Abstract

摘要：

以多糖为外壳的纳米银粒子(AgNPs)已被应用于病害防治、作物害虫管理和种子处理。壳寡糖(COS)是一种天然多糖,被认为是天然的植物免疫调节剂。此外,COS还被用作AgNPs的稳定剂。本研究探讨了壳寡糖-纳米银(COS-AgNPs)的合成、抗菌活性,及其作为种子处理剂对红花种子萌发和幼苗生长的影响。结果表明,COS与硝酸银反应后产生了431 nm 的吸收峰,且产物具有和COS相似的红外光谱特征峰,证明了COS-AgNPs被成功合成。COS-AgNPs的平均粒径为86 nm,具有典型的面心立方结构。体外抑菌试验表明,COS-AgNPs对木贼镰孢(Fusarium equiseti)、茄腐镰孢(Fusarium solani)、梨形毛霉(Mucor piriformis)的生长均有抑制作用,当COS-AgNPs的质量浓度为100 μg·mL^-1时,对这3种病原真菌的最高抑制率均超过73%。12.5 μg·mL^-1的COS-AgNPs处理能促进红花种子的萌发。此外,适宜质量浓度的COS-AgNPs处理还能增加红花幼苗的株高、根长、侧根数量和幼苗鲜重,促进红花幼苗的生长。

关键词: 壳寡糖, 纳米银, 抗菌活性, 红花, 种子萌发

Abstract:

Silver nanoparticles (AgNPs) coated with polysaccharides have been applied in disease control, crop pest management, and seed treatment. Chitooligosaccharide (COS), a natural polysaccharide, is recognized as a natural plant immune modulator. Additionally, COS has been utilized as a stabilizer for AgNPs. This study investigated the synthesis and antifungal activity of chitooligosaccharide-nano silver (COS-AgNPs), as well as their effects on safflower (Carthamus tinctorius L.) seed germination and seedling growth when used as a seed treatment agent. The results showed that a characteristic absorption peak at 431 nm was observed after the reaction between COS and silver nitrate, and the product exhibited infrared spectral characteristic peaks similar to those of COS, confirming the successful synthesis of COS-AgNPs. The average particle size of COS-AgNPs was 86 nm, and they possessed a typical face-centered cubic (FCC) structure. In vitro antifungal tests demonstrated that COS-AgNPs exerted inhibitory effects on the growth of Fusarium equiseti, Fusarium solani, and Mucor piriformis. When the mass concentration of COS-AgNPs was 100 μg·mL^-1, the maximum inhibition rates against these three pathogenic fungi all exceeded 73%. Treatment with 12.5 μg·mL^-1 COS-AgNPs could promote the germination of safflower seeds. Furthermore, treatment with an appropriate mass concentration of COS-AgNPs could increase the plant height, root length, number of lateral roots, and fresh weight of safflower seedlings, thereby promoting the growth of safflower seedlings.

Key words: chitooligosaccharide, nano silver, antifungal activity, safflower, seed germination

中图分类号:

原增艳, 任欣欣, 宋小锋, 朱畇昊. 壳寡糖-纳米银的抗菌活性及其对红花种子萌发和幼苗生长的影响[J]. 浙江农业学报, 2026, 38(1): 76-84.

YUAN Zengyan, REN Xinxin, SONG Xiaofeng, ZHU Yunhao. Antifungal activity of chitooligosaccharide-nano silver and its effects on safflower seed germination and seedling growth[J]. Acta Agriculturae Zhejiangensis, 2026, 38(1): 76-84.

图/表 5

图1 壳寡糖-纳米银(COS-AgNPs)的表征 A,壳寡糖(COS,左)、COS-AgNPs(右)的颜色;B,制备的COS-AgNPs的紫外-可见吸收光谱;C,制备的COS-AgNPs粒径;D,制备的COS-AgNPs的X射线衍射(XRD)图谱;E,制备的COS-AgNPs的傅里叶变换红外光谱(FTIR)。

Fig.1 Characterization of chitooligosaccharide-nano silver (COS-AgNPs) A, Color of chitooligosaccharide (COS, left) and COS-AgNPs (right); B, Ultraviolet-visible absorption spectrum of the prepared COS-AgNPs; C, Particle size of the prepared COS-AgNPs; D, X-ray diffraction (XRD) pattern of the prepared COS-AgNPs; E, Fourier transform infrared spectrum (FTIR) of the prepared COS-AgNPs.

图2 壳寡糖(COS)终质量浓度(A)、AgNO3终浓度(B)对壳寡糖-纳米银(COS-AgNPs)合成的影响

Fig.2 Effect of final mass concentration of chitooligosaccharides (COS) (A) and final AgNO3 concentration (B) on the synthesis of chitooligosaccharides-nano silver (COS-AgNPs)

表1 不同质量浓度的壳寡糖-纳米银(COS-AgNPs)对病原真菌的抑制率

Table 1 Inhibition rate of different mass concentrations of chitooligosaccharides-nano silver (COS-AgNPs) against pathogenic fungi

质量浓度/(μg·mL^-1) Mass cconcentration/(μg·mL^-1)	对不同菌株的抑制率/%Inhibition rate aginst different fungi strains/%
质量浓度/(μg·mL^-1) Mass cconcentration/(μg·mL^-1)	WY3	BZ-1	BZ-2
50	57.93±1.82 c	18.83±5.62 e	27.06±1.21 e
60	54.88±4.22 c	27.27±1.73 e	30.24±1.83 e
70	54.27±3.16 c	57.79±0.71 d	39.79±0.45 d
80	55.49±2.79 c	65.58±0.73 c	55.44±0.67 c
90	60.98±0.79 b	70.78±1.95 b	77.19±1.21 b
100	73.78±2.11 a	77.92±1.12 a	80.37±1.21 a

图3 不同质量浓度的壳寡糖-纳米银(COS-AgNPs)对红花种子萌发的影响同一时间柱上无相同字母的表示差异显著(p<0.05)。

Fig.3 Influence of chitooligosaccharides-nano silver (COS-AgNPs) mass concentration on germination rate of safflower seeds Bars marked without the same letters indicate significant difference at p<0.05 on the same day.

图4 不同质量浓度的壳寡糖-纳米银(COS-AgNPs)对红花种子生长的影响柱上无相同字母的表示差异显著(p<0.05)。

Fig.4 Influence of chitooligosaccharides-nano silver (COS-AgNPs) mass concentrations on growth of safflower seedlings Bars marked without the same letters indicate significant difference at p<0.05.

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壳寡糖-纳米银的抗菌活性及其对红花种子萌发和幼苗生长的影响

Antifungal activity of chitooligosaccharide-nano silver and its effects on safflower seed germination and seedling growth

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