聚集信息素和植物挥发物互作对豆大蓟马的行为调控作用

doi:10.3969/j.issn.1004-1524.20240731

浙江农业学报 ›› 2026, Vol. 38 ›› Issue (3): 532-540.DOI: 10.3969/j.issn.1004-1524.20240731

聚集信息素和植物挥发物互作对豆大蓟马的行为调控作用

李晓维(), 卢欣欣, 彭小华, 吕要斌()

浙江省农业科学院植物保护与微生物研究所, 农产品质量安全全国重点实验室, 浙江杭州 310021

收稿日期:2024-08-13 出版日期:2026-03-25 发布日期:2026-04-17
作者简介:^*吕要斌,E-mail: luybcn@163.com
李晓维,主要从事农业害虫综合防治研究。E-mail: lixiaowei1005@163.com
通讯作者: 吕要斌
基金资助:
浙江省“尖兵”“领雁”研发攻关计划(2022C02016);陕西省重点研发计划项目(2024NC-YBXM-047)

Behavioral regulation of the interaction between aggregation pheromone and plant volatiles on Megalurothrips usitatus

LI Xiaowei(), LU Xinxin, PENG Xiaohua, LYU Yaobin()

State Key Laboratory for Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2024-08-13 Published:2026-03-25 Online:2026-04-17
Contact: LYU Yaobin

摘要/Abstract

摘要：

豆大蓟马是豆科作物的重要害虫,给我国豆科作物种植产业造成巨大损失。基于昆虫信息素和植物挥发物的行为调控技术是豆大蓟马重要的绿色防控措施。本研究旨在筛选对豆大蓟马具有引诱作用的植物挥发物,以明确聚集信息素与植物挥发物互作对豆大蓟马的行为调控作用,并筛选聚集信息素和植物挥发物增效配方。结果表明,特定剂量的芳樟醇(0.1 μL·mL^-1)、异烟酸甲酯(1.0 μL·mL^-1)和丁香酚(1.0 μL·mL^-1)能够显著引诱豆大蓟马雌成虫。将上述剂量的3种挥发物分别与豆大蓟马聚集信息素(最佳引诱剂量5.00 ng·μL^-1的反式,反式-金合欢醇乙酸酯)混合后,芳樟醇和丁香酚对反式,反式-金合欢醇乙酸酯具有拮抗作用,而异烟酸甲酯则对反式,反式-金合欢醇乙酸酯没有拮抗作用,说明5.00 ng·μL^-1反式,反式-金合欢醇乙酸酯与1.0 μL·mL^-1异烟酸甲酯的组合可作为豆大蓟马引诱剂的优化配比。本研究筛选出了3种能够显著引诱豆大蓟马的植物挥发物,明确了植物挥发物与聚集信息素的相互作用,为基于信息化合物的豆大蓟马行为调控技术奠定了基础,同时为开发高效豆大蓟马引诱剂提供了依据。

关键词: 豆大蓟马, 聚集信息素, 植物挥发物, 行为调控

Abstract:

Megalurothrips usitatus is an important pest on leguminous crops, and has been reported to cause tremendous losses to leguminous crops industry in China. Aggregation pheromone and plant volatiles based behavioral manipulation techniques are promising eco-friendly control tactics for M. usitatus. The aim of this study was to screen plant volatiles with attractive activities to M. usitatus, investigate the interaction between aggregation pheromone and plant volatiles, and develop synergist blends. The results showed that 0.1 μL·mL^-1 linalool, 1.0 μL·mL^-1 methyl isonicotinate and 1.0 μL·mL^-1 eugenol were attractive to M. usitatus female adults. When the three volatile compounds at the specified doses were mixed with the aggregation pheromone components of M. usitatus at the optimal luring dose [5.00 ng·μL^-1 (E, E)-farnesyl acetate] respectively, eugenol and linalool showed antagonistic effects against (E,E)-farnesylacetate. By contrast, methyl isonicotinate showed no antagonistic effect on (E,E)-farnesylacetate, indicating that the combination of 5.00 ng·μL^-1 (E, E)-farnesyl acetate and 1.0 μL·mL^-1 methyl isonicotinate can be used as an optimized ratio for the bait of M. usitatus. This study screened out three plant volatiles which can significantly attract M. usitatus, clarified the interaction between plant volatiles and aggregation pheromone, which provided the basis for the behavior regulation technology of M. usitatus based on information technology and provided references for the development of efficient M. usitatus attractants.

Key words: Megalurothrips usitatus, aggregation pheromone, plant volatile, behavioral regulation

中图分类号:

S435

李晓维, 卢欣欣, 彭小华, 吕要斌. 聚集信息素和植物挥发物互作对豆大蓟马的行为调控作用[J]. 浙江农业学报, 2026, 38(3): 532-540.

LI Xiaowei, LU Xinxin, PENG Xiaohua, LYU Yaobin. Behavioral regulation of the interaction between aggregation pheromone and plant volatiles on Megalurothrips usitatus[J]. Acta Agriculturae Zhejiangensis, 2026, 38(3): 532-540.

图/表 5

图1 豆大蓟马雌成虫对不同剂量反式,反式-金合欢醇乙酸酯的嗅觉选择情况 **表示不同处理组与对照之间存在极显著(p<0.01)差异,NS表示没有统计学差异。下同。

Fig.1 Olfactory selection of M. usitatus female adults to different doses of (E,E)-farnesyl acetate ** indicates a highly significant (p<0.01) difference between the treatment group and the control, and NS indicates no statistically significant difference. The same as below.

图2 豆大蓟马雌成虫对不同剂量下5种植物挥发物的嗅觉选择情况 *、***表示不同处理组与对照之间存在显著(p<0.05)和极显著(p<0.001)差异,NS表示没有统计学差异。下同。

Fig.2 Olfactory selection of M. usitatus female adults to 5 plant volatiles at different doses * and ***indicate significant (p<0.05) and extremely significant (p<0.001) differences between treatment group and control, NS mean there was no significant difference. The same as below.

图3 豆大蓟马雌成虫对聚集信息素与丁香酚混合物的嗅觉选择行为 AP,聚集信息素;E,丁香酚。下同。

Fig.3 Olfactory selection of M. usitatus female adults to the mixture of aggregation pheromone compound and eugenol AP, Aggregation pheromone compound; E, Enugenol. The same as below.

图4 豆大蓟马雌成虫对聚集信息素与芳樟醇混合物的嗅觉选择行为 L,芳樟醇。

Fig.4 Response of female adults of Megalurothrips usitatus to the mixture of aggregation pheromone compound and linalool L, Linalool.

图5 豆大蓟马雌成虫对聚集信息素与异烟酸甲酯混合物的嗅觉选择行为 M,异烟酸甲酯。

Fig.5 Response of M. usitatus female adults to the mixture of aggregation pheromone compound and methyl isonicotinate M, Methyl isonicotinate.

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聚集信息素和植物挥发物互作对豆大蓟马的行为调控作用

Behavioral regulation of the interaction between aggregation pheromone and plant volatiles on Megalurothrips usitatus

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