Polymorphism of SLA-DQB exon 2 and its association with diarrhea in Yantai black pigs

doi:10.3969/j.issn.1004-1524.2016.10.07

›› 2016, Vol. 28 ›› Issue (10): 1671-1677.DOI: 10.3969/j.issn.1004-1524.2016.10.07

• Animal Science • Previous Articles Next Articles

Polymorphism of SLA-DQB exon 2 and its association with diarrhea in Yantai black pigs

MU Tong, WANG Guo-mei, YANG Qi-rui, JIN Li, ZHANG Hai-long, ZHANG Li, TIAN Xiao-jing, LIU Li-xia^*

College of Life Science and Engineering, Northwest University for Nationalities, Lanzhou 730030, China

Received:2016-03-05 Online:2016-10-15 Published:2016-11-20

Abstract

Abstract: Polymorphism of SLA-DQB exon 2 in Yantai black pigs was studied using PCR-RFLP, and its correlation with piglets diarrhea was analyzed. The results showed that there were four alleles (A, B, C, D) and seven RFLP genotypes (AA, BB, CC, AB, AC, BC, AD), polymorphism of the DQB exon 2 was abundant. The chi-square test displayed DQB exon 2 of Yantai black pigs deviated Hardy-Weinberg equilibrium(P<0.05). The results of least squares showed that the sex of Yantai black pig and genotype of DQB exon 2 did not have significant influence on diarrhea piglets (P>0.05). However, polymorphism of DQB exon 2 in different genders affected diarrhea piglets (P<0.01). In males, diarrhea score of genotype AD was higher than that of AA genotype (P<0.05), in female, scores of BB, AC, BC, CC were higher than others (P<0.05).

Key words: Yantai black pig, SLA-DQB, piglet diarrhea, PCR-RFLP, polymorphism

CLC Number:

S858.28

MU Tong, WANG Guo-mei, YANG Qi-rui, JIN Li, ZHANG Hai-long, ZHANG Li, TIAN Xiao-jing, LIU Li-xia. Polymorphism of SLA-DQB exon 2 and its association with diarrhea in Yantai black pigs[J]. , 2016, 28(10): 1671-1677.

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Polymorphism of SLA-DQB exon 2 and its association with diarrhea in Yantai black pigs

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