HMW-GS composition analysis of core wheat breeding parents in Southern Zhejiang region

doi:10.3969/j.issn.1004-1524.2018.12.03

›› 2018, Vol. 30 ›› Issue (12): 2001-2006.DOI: 10.3969/j.issn.1004-1524.2018.12.03

• Crop Science • Previous Articles Next Articles

HMW-GS composition analysis of core wheat breeding parents in Southern Zhejiang region

LIU Yong'an^1,2, PAN Binrong^1,2, YUE Gaohong^1,2, MEI Xixue^1,2, XU Likui^1,2,*, ZHANG Zongchen^1,2, ZHOU Zhihui^1,2

1. Key Laboratory of Crop Breeding South Zhejiang, Wenzhou 325006, China;
2. Crop Research Institute, Wenzhou Academy of Agricultural Science, Wenzhou 325006, China

Received:2018-03-29 Online:2018-12-25 Published:2018-12-28

Abstract

Abstract: In order to learn the genetic basis of quality of the core wheat breeding parents in Southern Zhejiang region, the composition of high molecular weight glutenin subunit of twenty-eight core cultivars (lines) was analyzed using the method of SDS-PAGE. The results showed that there were Null (57.14%) and 1 (42.86%) subunit alleles on Glu-A1 loci; 7+8 (67.86%), 7+9 (25.00%), 17+18 (3.57%) and 13+16 (3.57%) subunit alleles on Glu-B1 loci; 2+12 (96.43%) and 5+10 (3.57%) subunit alleles on Glu-D1 loci. On the other hand, there were five kind of HMW-GS combinations, 1/7+8/2+12 (39.29%), Null/7+8/2+12 (28.57%), Null/7+9/2+12 (25.00%), 1/17+18/5+10 (3.57%) and Null/13+16/2+12 (3.57%), respectively.

Key words: wheat, Southern Zhejiang region, HMW-GS

CLC Number:

S512

LIU Yong'an, PAN Binrong, YUE Gaohong, MEI Xixue, XU Likui, ZHANG Zongchen, ZHOU Zhihui. HMW-GS composition analysis of core wheat breeding parents in Southern Zhejiang region[J]. , 2018, 30(12): 2001-2006.

References

[1] OSBORNE T B.The proteins of the wheat kernel[M]. Washington D C: Carnegie Institute of Washington Publication, 1907.
[2] PAYNE P I.Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality[J]. Annual Review of Plant Physiology and Plant Molecular Biology, 1987, 38(1):141-153.
[3] SHEWRY P R, HALFORD N G, TATHAM A S.High molecular weight subunits of wheat glutenin[J]. Journal of Cereal Science, 1992, 15(2):105-120.
[4] 宋建民, 刘爱峰, 李豪圣, 等. 小麦麦谷蛋白与面包烘烤品质关系研究进展[C]//中国青年农业科学学术年报. 北京: 中国农业科学技术出版社, 2004: 84-89.
[5] 毛沛, 李宗智, 卢少源. 小麦遗传资源HMW麦谷蛋白亚基组成及其与面包烘烤品质关系的研究[J]. 中国农业科学, 1995, 28(增刊1): 22-27.
MAO P, LI Z Z, LU S Y.The composition of high molecular weight glutenin subunits of genetic resources of bread wheat and their relationship with bread-making quality[J]. Scientia Agricultura Sinica, 28(Suppl. 1): 22-27. (in Chinese with English abstract)
[6] 范玉顶, 李斯深, 孙海艳, 等. HMW-GS与北方手工馒头加工品质关系的研究[J]. 作物学报, 2005, 31(1): 97-101.
FAN Y D, LI S S, SUN H Y, et al.Relationship between HMW-GS and making quality of northern style hand-made steamed bread[J]. Acta Agronomica Sinica, 2005, 31(1): 97-101. (in Chinese with English abstract)
[7] 张影全, 魏益民, 张波, 等. 小麦高分子量谷蛋白亚基(HMW-GS)对面条感官质量的影响[J]. 中国农业科学, 2013, 46(1): 121-129.
ZHANG Y Q, WEI Y M, ZHANG B, et al.Influence of high molecular weight glutenin subunits (HMW-GS) on sensory quality properties of Chinese dry noodle[J]. Scientia Agricultura Sinica, 2013, 46(1): 121-129. (in Chinese with English abstract)
[8] 刘广田, 许明辉. 普通小麦胚乳谷蛋白亚基的遗传研究[J]. 中国农业科学, 1988, 21(1): 56-60.
LIU G T, XU M H.The inheritance of endosperm glutenin subunit in comon wheat (Triticum aestivum L.)[J]. Scientia Agricultura Sinica, 1988, 21(1): 56-60. (in Chinese with English abstract)
[9] 张丽琴, 刘春雷, 杨雪, 等. 黄淮麦区小麦新品种(系)高分子量谷蛋白亚基多态性分析[J]. 麦类作物学报, 2012, 32(1): 79-82.
ZHANG L Q, LIU C L, YANG X, et al.Polymorphism analysis of HMW-GS in new wheat varieties in Huang-huai Wheat Region of China[J]. Journal of Triticeae Crops, 2012, 32(1): 79-82. (in Chinese with English abstract)
[10] 徐黎黎, 颜泽洪, 魏育明, 等. 东方小麦高分子量谷蛋白亚基组成分析[J]. 四川农业大学学报, 2005, 23(2): 137-141.
XU L L, YAN Z H, WEI Y M, et al.Analysis of HMW glutenin subunits in Triticum turanicum Jakubz.[J]. Journal of Sichuan Agricultural University, 2005, 23(2): 137-141. (in Chinese with English abstract)
[11] 吴远双, 宋玉竹. 一种考马斯亮蓝G-250快速染色法在SDS-PAGE中的应用[J]. 光谱实验室, 2013, 30(6): 3109-3113.
WU Y S, SONG Y Z.Application of a fast protein staining method with G-250 in SDS-PAGE[J]. Chinese Jounaral of Spectroscopy Laboratory, 2013, 30(6): 3109-3113. (in Chinese with English abstract)
[12] 郑玉姝, 赵朴, 赵宏坤. SDS-PAGE染色-脱色方法的改良[J]. 实验室研究与探索, 2007, 26(1): 41-42.
ZHENG Y S, ZHAO P, ZHAO H K.An improved method for staining-destaining of SDS-PAGE[J]. Research and Exploration in Laboratory, 2007, 26(1): 41-42. (in Chinese with English abstract)
[13] PAYNE P I, LAWRENCE G J.Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaplold wheat[J]. Cereal Research Communications, 1983, 11: 29-35.
[14] 张志清, 郑有良, 魏育明, 等. 四川主栽小麦品种高分子量谷蛋白亚基遗传变异分析[J]. 麦类作物学报, 2002, 22(1): 14-18.
ZHANG Z Q, ZHENG Y L, WEI Y M, et al.Genetic variation of HMW-GS among Sichuan wheat cultivars[J]. Journal of Triticeae Crops, 2002, 22(1): 14-18. (in Chinese with English abstract)
[15] 陈晓杰, 王亚娟, 申磊, 等. 西北春麦区小麦地方品种高分子量麦谷蛋白亚基组成分析[J]. 植物遗传资源学报, 2009, 10(1): 42-45.
CHEN X J, WANG Y J, SHEN L, et al.HMW-GS diversity of wheat landraces in northwest spring wheat production area[J]. Journal of Plant Genetic Resources, 2009, 10(1): 42-45. (in Chinese with English abstract)
[16] 郭志富, 衣莹, 龙翔宇, 等. 东北春小麦及部分外引小麦高分子量谷蛋白亚基组成分析[J]. 华北农学报, 2008, 23(2): 72-76.
GOU Z F, YI Y, LONG X Y, et al.Analysis on high-molecular-weight glutenin subunit composition of spring wheat in northeast China and introduced wheat[J]. Acta Agriculturae Boreali-Sinica, 2008, 23(2): 72-76. (in Chinese with English abstract)
[17] 王红梅, 厚毅清, 张艳萍, 等. 甘肃小麦部分种质高分子量麦谷蛋白亚基(HMWGS)遗传变异分析[J]. 分子植物育种, 2009, 7(5): 873-878.
WANG H M, HOU Y Q, ZHANG Y P, et al.Genetic diversity of the collected wheat germplasms in Gansu Province revealed based on HMWGS variation by SDS-PAGE[J]. Molecular Plant Breeding, 2009, 7(5): 873-878. (in Chinese with English abstract)
[18] 杨子博, 顾正中, 周羊梅, 等. 江苏淮北地区小麦品种资源高分子量谷蛋白亚基的组成分析[J]. 西南农业学报,2015, 28(4): 1465-1469.
YANG Z B, GU Z Z, ZHOU Y M, et al.Composition analysis of HMW-GS in wheat cultivars from Huaibei Region of Jiangsu Province[J]. Southwest China Journal of Agricultural Sciences, 2015, 28(4): 1465-1469. (in Chinese with English abstract)
[19] 刘永安, 潘彬荣, 岳高红, 等. 小麦优质亚基和抗白粉病Pm4a基因聚合体的PCR鉴定[J]. 浙江农业学报, 2016, 28(4): 545-549.
LIU Y A, PAN B R, YUE G H, et al.PCR identification of the wheat polymers with high quality subunit and powdery mildew resistance Pm4a gene[J]. Acta Agriculturae Zhejiangensis, 2016, 28(4): 545-549. (in Chinese with English abstract)
[20] 苏东民. 中国馒头分类专家咨询调查研究[J]. 粮食科技与经济, 2006 (5): 49-51.
SU D M.Investigation on the classification of steamed bread in China[J]. Grain Science and Technology and Economy, 2006 (5): 49-51. (in Chinese)
[21] 施俊生, 王仁杯. 浙江省小麦品种结构演变的比较分析[J]. 种子世界, 2016 (5): 19-21.
SHU J S, WANG R B.Analysis on the evolution of wheat varieties in Zhejiang Province[J]. Seed World, 2016 (5): 19-21. (in Chinese)

HMW-GS composition analysis of core wheat breeding parents in Southern Zhejiang region

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