Effects on accumulation of cadmium and antioxidant system of different Morchella spp. under cadmium stress

doi:10.3969/j.issn.1004-1524.20221550

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (10): 2321-2331.DOI: 10.3969/j.issn.1004-1524.20221550

• Horticultural Science • Previous Articles Next Articles

Effects on accumulation of cadmium and antioxidant system of different Morchella spp. under cadmium stress

FAN Liying¹^,²(), FAN Tingting², TONG Zongjun³, LIANG Liyun², ZHAO Zhiyong², CHEN Hui³, ZHOU Changyan², ZHAO Xiaoyan²^,^*()

1. School of Health Sciences and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
2. Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
3. Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China

Received:2022-11-03 Online:2023-10-25 Published:2023-10-31

Abstract

Abstract:

Morchella spp., as a kind of edible fungus cultivated in soil, faces the risk of exposure and accumulation of cadmium (Cd) in the soil environment. In this study, the commonly used cultivars Morchella sextelata M25 and Morchella importuna I15 were selected as test materials to compare and analyze the effects of Cd stress on mycelium physiology and Cd accumulation, specifically including the differences in mycelial growth rate, biomass, microstructure, antioxidant enzymes, antioxidant active substances and Cd content. The results showed that I15 had stronger ability of Cd accumulation than M25. The catalase (CAT), glutathione reductase (GR), ascorbate peroxidase (APX) enzyme activities and malondialdehyde (MDA), ascorbic acid (AsA) contents of the different Morchella spp. showed a trend of first increasing and then decreasing with the increase of Cd concentration, but the GR, glutathione peroxidase (GSH-Px) enzyme activities and glutathione (GSH) contents of I15 were higher than M25. Combining the physiology and Cd accumulation capacity of the different Morchella spp., it found that I15 had stronger accumulation ability, better grow, less damage under Cd stress than M25, also had stronger defense ability against cell damage in the glutathione transformation system. The results of this study not only laid the foundation for the screening of Morchella spp. Cd-resistant strains and the innovation of Cd-containing soil cultivation models, but also provided new insights for fungal bioremediation.

Key words: Morchella, cadmium stress, accumulation, antioxidant enzymes, glutathione transformation system

CLC Number:

S646.9

FAN Liying, FAN Tingting, TONG Zongjun, LIANG Liyun, ZHAO Zhiyong, CHEN Hui, ZHOU Changyan, ZHAO Xiaoyan. Effects on accumulation of cadmium and antioxidant system of different Morchella spp. under cadmium stress[J]. Acta Agriculturae Zhejiangensis, 2023, 35(10): 2321-2331.

Figures/Tables 6

Fig.1 Effects of Cd stress on the growth rate, biomass, growth morphology, and microscopic morphology of the different Morchella spp. Mycelia I15, M.importuna; M25, M.sextelata M25. *, ** and *** mena significant differences at the levels of 0.05, 0.01 and 0.001 respectiviely, ns means not significant. The same as below.

Fig.2 Effects of Cd stress on the Cd bioconcentration factor of the different Morchella spp. mycelia Data on the bars marked without the same lowercase letter indicated significant differences at P<0.05.

Table 1 Mathematical model of relationship between Cd contents in culture medium and the different Morchella spp. mycelia

菌株 Strain	回归方程 regression equation	决定系数 R²
I 15	y=1.797/(1+e^2.891-6.187^x)	0.954 7
M25	y=3.271/(1+e^3.232-2.582^x)	0.983 3

Fig.3 Effects of Cd stress on MDA content in the different Morchella spp. Mycelia

Fig.4 Effects of Cd stress on activities of SOD, CAT, APX, GR and GSH-Px in the mycelia of different Morchella spp.

Fig.5 Effects of Cd stress on the content of GSH and AsA in the mycelia of the different Morchella spp.

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Effects on accumulation of cadmium and antioxidant system of different Morchella spp. under cadmium stress

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