Oxidative stress response and biosorption capacity of Aspergillus flavus TL-F3 under nano silver particles stress

doi:10.3969/j.issn.1004-1524.2023.01.14

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (1): 128-137.DOI: 10.3969/j.issn.1004-1524.2023.01.14

• Plant Protection • Previous Articles Next Articles

Oxidative stress response and biosorption capacity of Aspergillus flavus TL-F3 under nano silver particles stress

JI Yangyang(), ZHANG Peng, LIU Xiaoqiang, DONG Zhenru, ZHANG Wei, FAN Ting()

Anhui Province Key Lab of Farmland Ecological Conservation and Pollution Prevention, College of Resources and Environment,Anhui Agricultural University, Hefei 230036, China

Received:2022-01-19 Online:2023-01-25 Published:2023-02-21

Abstract

Abstract:

In order to study the changes of oxidative stress of Aspergillus flavus TL-F3 and its adsorption capacity of nano silver particles (AgNPs) under AgNPs stress, a sequential batch experiment was carried out. The effects of AgNPs on the growth, cell morphology, extracellular polymer (EPS) secretion, antioxidant enzymes and adsorption capacity of A. flavus TL-F3 were investigated. It was shown that AgNPs could inhibit the growth of A. flavus TL-F3, and the inhibitory effects were positively correlated with the concentration of AgNPs. Scanning electron microscope analysis showed that, the hyphae of A. flavus TL-F3 was incompletely and the surface was damaged under AgNPs stress. Compared with the control group (without AgNPs),when the AgNPs concentration was 80 mg·L^-1, the EPS content of A. flavus TL-F3 rose to 237.55 mg·g^-1, and malondialdehyde content, superoxide dismutase activity and reduced glutathione peptide content were significantly (P<0.05) increased, and Na⁺K⁺-ATP enzyme activity was significantly (P<0.05) decreased by 50.37%. A. flavus TL-F3 had a certain adsorption capacity for AgNPs, and when the concentration of AgNPs was 1, 30, 50, 80 mg·L^-1, the adsorption rate for AgNPs could reach 68.54% and above. In conclusion, A. flavus TL-F3 could be used for removing Ag and AgNPs from contaminated water bodies.

Key words: nano silver, Aspergillus flavus, toxic effect, oxidative stress, biosorption

CLC Number:

X522

JI Yangyang, ZHANG Peng, LIU Xiaoqiang, DONG Zhenru, ZHANG Wei, FAN Ting. Oxidative stress response and biosorption capacity of Aspergillus flavus TL-F3 under nano silver particles stress[J]. Acta Agriculturae Zhejiangensis, 2023, 35(1): 128-137.

Figures/Tables 7

Fig.1 Transmission electron microscope (TEM) image of AgNPs and its ultraviolet wavelength scan result (B)

Fig.2 Effect of AgNPs on growth of A. flavus TL-F3

Fig.3 Influence of AgNPs on morphology of A. flavus TL-F3 A, Scanning electron microscope (SEM) image of control group without AgNPs; B, SEM image of experiment group with 80 mg·L-1 AgNPs; C, Energy spectra of control group; D, Energy spectra of experiment group; E, F, Element mapping analysis diagram of control group; G, H, Element mapping analysis diagram of experiment group.

Fig.4 Content (A) and toxicity (B) of Ag+ released from AgNPs on A. flavus TL-F3 Bars marked without the same letters indicated significant difference at P<0.05. The same as below.

Fig.5 Effect of AgNPs on secretion of EPS by A. flavus TL-F3 (A) and changes of particle size before and after interaction of AgNPs with A. flavus TL-F3 (B)

Fig.6 Effect of AgNPs on activity of Na+K+-ATP enzyme (A), MDA content (B), SOD activity (C) and GSH content (D) of A. flavus TL-F3

Fig.7 Changes of ultraviolet absorption peak intensity before and after the interaction of A. flavus TL-F3 with AgNPs (A) and adsorption rate of AgNPs by A. flavus TL-F3 (B) 1-0 and 1-7 indicated interaction of 1 mg·L-1 AgNPs with A. flavus for 0 and 7 days, respectively. The rest may be deduced by analogy.

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Oxidative stress response and biosorption capacity of Aspergillus flavus TL-F3 under nano silver particles stress

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