Inactivation efficiency of various disinfectants against African swine fever virus evaluated by PMA-qPCR

doi:10.3969/j.issn.1004-1524.2023.02.07

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (2): 301-307.DOI: 10.3969/j.issn.1004-1524.2023.02.07

• Animal Science • Previous Articles Next Articles

Inactivation efficiency of various disinfectants against African swine fever virus evaluated by PMA-qPCR

YANG Yue¹(), SHAO Jing², ZHANG Pengfei¹, CHEN Dishi², CHEN Wanting¹, WANG Yin¹^,³^,^*(), LUO Yan¹^,³, YANG Zexiao¹^,³, YAO Xueping¹^,³, REN Meishen¹^,³

1.College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
2. Sichuan Animal Disease Prevention and Control Center, Chengdu 611130, China
3. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China

Received:2022-04-01 Online:2023-02-25 Published:2023-03-14
Contact: WANG Yin

Abstract

Abstract:

In order to evaluate the biosecurity prevention and control level of large-scale pig farms, this study intends to establish a method to detect the infectivity of African swine fever virus(ASFV), and use this method to detect the inactivation effect of various disinfectants on ASFV. We established a method called PMA-qPCR, which was used to evaluate the inactivation effect after treating ASFV with different kinds of disinfectants, and used it to explore the best treatment method of disinfectants. The results of PMA-qPCR showed that ASFV had different sensitivities to different types of disinfectants, with the highest sensitivity to potassium hydrogen persulfate complex solution, which could effectively inactivate ASFV within 20 min after diluting at 0.5%. The results had certain reference for the disinfection of biosecurity.

Key words: African swine fever virus, disinfectant, propidium monoazide, biosecurity

CLC Number:

S851.36

YANG Yue, SHAO Jing, ZHANG Pengfei, CHEN Dishi, CHEN Wanting, WANG Yin, LUO Yan, YANG Zexiao, YAO Xueping, REN Meishen. Inactivation efficiency of various disinfectants against African swine fever virus evaluated by PMA-qPCR[J]. Acta Agriculturae Zhejiangensis, 2023, 35(2): 301-307.

Figures/Tables 8

References 18

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名称Name	序列Sequence (5'→3')
ASFV-F	CTGCTCATGGTATCAATCTTATCGA
ASFV-R	GATACCACAAGATCAGGCCGT
ASFV-P	FAM-CCACGGGAGGAATACCAACCCAGTG-TAMRA

名称Name	序列Sequence (5'→3')
ASFV-F	CTGCTCATGGTATCAATCTTATCGA
ASFV-R	GATACCACAAGATCAGGCCGT
ASFV-P	FAM-CCACGGGAGGAATACCAACCCAGTG-TAMRA

组别 Group	处理方式 Treatment
1	高压蒸汽灭菌(103.4 kPa,121℃),添加PMA Autoclaved (103.4 kPa,121 ℃)+PMA
2	常温处理,添加PMA Room temperature treatment+PMA
3	高压蒸汽灭菌(103.4 kPa,121℃),不添加PMA Autoclaved (103.4 kPa,121 ℃)
4	常温处理,不添加PMA Room temperature treatment

组别 Group	处理方式 Treatment
1	高压蒸汽灭菌(103.4 kPa,121℃),添加PMA Autoclaved (103.4 kPa,121 ℃)+PMA
2	常温处理,添加PMA Room temperature treatment+PMA
3	高压蒸汽灭菌(103.4 kPa,121℃),不添加PMA Autoclaved (103.4 kPa,121 ℃)
4	常温处理,不添加PMA Room temperature treatment

组别 Group	处理方式 Treatment	Ct值 Ct value
1	高压蒸汽灭菌(103.4 kPa,121℃),添加PMA Autoclaved(103.4 kPa,121 ℃)+PMA	>40
2	常温处理,添加PMA Room temperature treatment+PMA	31.03
3	高压蒸汽灭菌(103.4 kPa,121℃),不添加PMA Autoclaved (103.4 kPa, 121 ℃)	32.27
4	常温处理,不添加PMA Room temperature treatment	30.69

Inactivation efficiency of various disinfectants against African swine fever virus evaluated by PMA-qPCR

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Figures/Tables 8

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组别 Groups	消毒剂稀释度与种类 Concentrations and types of disinfectants	灭活率 Inactivation rate/%
1	0.5%过硫酸氢钾复合物 0.5% Potassium hydrogen persulfate complex	99.87
2	1%二氯异氰脲酸钠 1% Sodium dichloroisocyanurate	98.14
3	2.5%二氧化氯 2.5% Chlorine dioxide	97.46
4	0.2%复合碘 0.2% Compound iodine	96.45
5	0.5%辛硫磷0.5% Phoxim	81.75
6	0.2%复合戊二醛季铵盐溶液 0.2% Compound glutaraldehyde quaternary ammonium salt solution	73.51
7	1%有机酸 1% Organic acids	67.83
8	0.5%溴化二甲基二癸基羟胺 0.5% Dimethyl didecyl hydroxylamine bromide	65.44
9	0.25%戊二醛-癸甲溴铵 0.25% Glutaraldehyde decamethonium bromide	62.84