Genetic diversity analysis of three Opsariichthys bidens populations in Zhejiang Province based on mitochondrial Cyt b gene sequences

doi:10.3969/j.issn.1004-1524.2023.02.06

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (2): 293-300.DOI: 10.3969/j.issn.1004-1524.2023.02.06

• Animal Science • Previous Articles Next Articles

Genetic diversity analysis of three Opsariichthys bidens populations in Zhejiang Province based on mitochondrial Cyt b gene sequences

LIU Shili¹^,²(), LIAN Qingping¹^,², JIA Yongyi¹, CHI Meili¹, LI Fei¹, JIANG Jianhu¹, LIU Yinuo¹, ZHENG Jianbo¹, CHENG Shun¹, GU Zhimin¹^,^*()

1. Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, Zhejiang, China
2. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China

Received:2021-11-05 Online:2023-02-25 Published:2023-03-14
Contact: GU Zhimin

Abstract

Abstract:

To further understand the status of germplasm resources and impact of artificial reproduction on population genetic structure of Opsariichthys bidens in Zhejiang Province, the mitochondrial Cyt b gene of Qiantang River (QT), Oujiang (OJ), and a cultured population from Balidian (BL) were amplified and determined. The 1 140 bp Cyt b gene sequence was obtained, and analyzed with the sequences of 236 individuals in GenBank. In these sequences, 288 variation sites were detected and 136 haplotypes were defined. The haplotype numbers of OJ, QT and BL populations were 1, 10 and 6, respectively. The haplotype diversity and nucleotide diversity of QT population were 0.716 and 0.016 16 respectively, while that of BL population were 0.607 and 0.012 05. The molecular variance analysis (AMOVA) showed that the genetic differences in Opsariichthys bidens were primarily derived from inter populations (55.06%) and minority from intra populations (44.94%), there was significant genetic differentiation among these populations (P<0.05). The stock of BL population came from QT, but its genetic diversity decreased after several generations of artificial reproduction. Phylogenetic tree constructed by the neighbor-joining method demonstrated that QT population could be divided into three branches: one branch was clustered with the haplotypes collected from Hunan of the Yangtze River system, and 63.64% of QT individuals belonged to this branch; the second branch was clustered with the haplotypes collected from Guangxi of the Pearl River System, 9.38% of the individuals belonged to this branch, and the remaining samples of QT and all the samples collected from OJ of Zhejiang were clustered into the third branch. This indicates that the ancestral origin of QT population is complex. This study provides reference for the protection and utilization of its germplasm resources.

Key words: Opsariichthys bidens, Cyt b, genetic diversity

CLC Number:

S965

LIU Shili, LIAN Qingping, JIA Yongyi, CHI Meili, LI Fei, JIANG Jianhu, LIU Yinuo, ZHENG Jianbo, CHENG Shun, GU Zhimin. Genetic diversity analysis of three Opsariichthys bidens populations in Zhejiang Province based on mitochondrial Cyt b gene sequences[J]. Acta Agriculturae Zhejiangensis, 2023, 35(2): 293-300.

Figures/Tables 7

References 20

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群体 Population	碱基Base
群体 Population	A	G	T	C	A+T	C+G
瓯江Oujiang	24.3	16.7	29.5	29.6	53.8	46.3
钱塘江Qiantang River	24.2	16.7	29.4	29.7	53.6	46.4
八里店Balidian	24.4	16.6	29.6	29.5	54.0	46.1

群体 Population	碱基Base
群体 Population	A	G	T	C	A+T	C+G
瓯江Oujiang	24.3	16.7	29.5	29.6	53.8	46.3
钱塘江Qiantang River	24.2	16.7	29.4	29.7	53.6	46.4
八里店Balidian	24.4	16.6	29.6	29.5	54.0	46.1

单倍型 Haplotype	瓯江 Oujiang	钱塘江 Qiantang River	八里店 Balidian	总计 Total
Hap1	33	0	0	33
Hap2	0	17	1	18
Hap3	0	5	19	24
Hap4	0	1	0	1
Hap5	0	1	0	1
Hap6	0	3	0	3
Hap7	0	2	0	2
Hap8	0	1	0	1
Hap9	0	1	0	1
Hap10	0	1	0	1
Hap11	0	1	3	4
Hap12	0	0	7	7
Hap13	0	0	1	1
Hap14	0	0	1	1

单倍型 Haplotype	瓯江 Oujiang	钱塘江 Qiantang River	八里店 Balidian	总计 Total
Hap1	33	0	0	33
Hap2	0	17	1	18
Hap3	0	5	19	24
Hap4	0	1	0	1
Hap5	0	1	0	1
Hap6	0	3	0	3
Hap7	0	2	0	2
Hap8	0	1	0	1
Hap9	0	1	0	1
Hap10	0	1	0	1
Hap11	0	1	3	4
Hap12	0	0	7	7
Hap13	0	0	1	1
Hap14	0	0	1	1

群体 Population	样本数/个 Sample size	变异位点/个 Variable sites	单倍型数/个 Number of haplotypes	单倍型多样性 Haplotype diversity (h)	核苷酸多样性 Nucleotide diversity (π)	Tajima’s D	P	Fu’s Fs	P
瓯江Oujiang	33	0	1	0	0	0	1.000	0	N.A.
钱塘江	33	52	10	0.716	0.016 16	1.610 20	0.925	9.297 38	0.999
Qiantang River
八里店Balidian	32	51	6	0.607	0.012 05	0.230 24	0.637	12.905 59	1.000
总体Total	334	288	130	0.968	0.060 17

Genetic diversity analysis of three Opsariichthys bidens populations in Zhejiang Province based on mitochondrial Cyt b gene sequences

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变异来源 Source of variation	自由度 Degree of freedom	方差总和 Sum of squares	变异组分 Variance components	变异百分比 Percentage of variation /%	遗传分化指数 Fst
群体间Among populations	2	434.558	6.489 89 Va	55.06	0.550 55
群体内Within populations	95	503.319	5.298 10 Vb	44.94
总计Total	97	937.878	11.787 99

群体 Population	瓯江 Oujiang	钱塘江 Qiantang River	八里店 Balidian
瓯江Oujiang		0.696 61	0.504 80
钱塘江Qiantang River	0.027 505		0.402 95
八里店Balidian	0.012 072	0.024 195

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