Comparison of purine alkaloids and catechin components of wild Camellia tachangensis in Pu’an County and Panzhou County, Guizhou Province, China

doi:10.3969/j.issn.1004-1524.20230972

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (6): 1232-1244.DOI: 10.3969/j.issn.1004-1524.20230972

• Crop Science • Previous Articles Next Articles

Comparison of purine alkaloids and catechin components of wild Camellia tachangensis in Pu’an County and Panzhou County, Guizhou Province, China

YANG Chun¹(), YANG Daixing¹^,², SU Shengfeng³, LIANG Sihui¹, LI Yan¹, GUO Yan¹, QIAO Dahe¹, MI Xiaozeng¹, CHEN Zhengwu¹^,^*()

1. Tea Research Institute,Guizhou Academy of Agricultural Sciences, Guiyang 550006, China
2. Tea College, Guizhou University, Guiyang 550025, China
3. Rural Work Center of Yuni Township, Panzhou County, Panzhou 553523, Guizhou, China

Received:2023-08-16 Online:2024-06-25 Published:2024-07-02

Abstract

Abstract:

The objective of this study was not only to explore the biochemical differences between wild Camellia tachangensis in Pu’an County and Panzhou County, but also to provide the scientific basis for the protection, development and utilization of wild C. tachangensis in Guizhou. A total of 40 C. tachangensis germplasm resources from Pu’an County and Panzhou County were used as materials, the contents of theobromine, theophylline, theacrine, caffeine,gallic acid, epigallocatechin (EGC), catechin (C), epicatechin (EC), epigallocatechin gallate (EGCG), gallocatechin gallate (GCG) and epigallocatechin gallate (ECG) were determined by high performance liquid chromatography (HPLC). Cluster analysis and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to classify C. tachangensis germplasm resources from two regions and select key differential compounds. The results showed that the main purine alkaloids in C. tachangensis from Pu’an and Panzhou were caffeine and theacrine, respectively. ECG and EC were the prominent components of catechin in C. tachangensis.The contents of theacrine, caffeine, gallic acid, C and EC in C. tachangensis from two regions were significantly different. The contents of caffeine and gallic acid in C. tachangensis form Pu’an were average to 28.53 mg·g^-1 and 0.29 mg·g^-1, which were significantly higher than those from Panzhou. The contents of theacrine, C and EC in C. tachangensis from Panzhou were average to 22.88 mg·g^-1, 2.37 mg·g^-1 and 36.83 mg·g^-1, which were extremely significantly higher than those from Pu’an. The correlation analysis showed that there were strong correlation between 11 biochemical components, with 14 pairs showing significant or extremely significant positive correlations, 16 pairs showing significant or extremely significant negative correlations. Based on the hierarchical clustering of 11 biochemical components, 40 C. tachangensis germplasm resources could be divided into 3 groups. All 30 C. tachangensis germplasm resources from Panzhou were composed group Ⅰ and the main biochemical characteristics were high content of theacrine and C, and low content of caffeine; 10 C. tachangensis germplasm resources from Pu’an were separated into 2 categories, GT-PA-03 and other 6 germplasm resources constituted group Ⅱ,the main biochemical characteristics were high content of caffeine, EC, ECG, and low content of theacrine, gallic acid, EGC, C, EGCG; GT-PA-01, GT-PA-02 and GT-PA-09 were composed group Ⅲ and the main biochemical characteristics were high content of gallic acid, EGC and EGCG. OPLS-DA results showed that caffeine, theacrine and gallic acid were the key biochemical factors to distinguish C. tachangensis germplasm resources from Pu’an and Panzhou. A series of specific genetic resources were screened out from the 40 C. tachangensis germplasm resources, which included three high theacrine (>25.0 mg·g^-1) germplasm resources, two high GCG (>15.0 mg·g^-1) germplasm resources, three high ECG (>80.0 mg·g^-1) germplasm resources, three high EC (>50.0 mg·g^-1) germplasm resources.

Key words: Pu’an, Panzhou, Camellia tachangensis, theacrine, caffeine

CLC Number:

S571.1

YANG Chun, YANG Daixing, SU Shengfeng, LIANG Sihui, LI Yan, GUO Yan, QIAO Dahe, MI Xiaozeng, CHEN Zhengwu. Comparison of purine alkaloids and catechin components of wild Camellia tachangensis in Pu’an County and Panzhou County, Guizhou Province, China[J]. Acta Agriculturae Zhejiangensis, 2024, 36(6): 1232-1244.

Figures/Tables 8

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材料编号	可可碱含量	茶碱含量	苦茶碱含量	咖啡碱含量
Material ID	Theobromine content	Theophylline content	Theacrine content	Caffeine content
GT-PA-01	14.18±0.62	0.48±0.01	N.D.	25.85±1.13
GT-PA-02	6.88±0.14	0.57±0.03	N.D.	30.04±0.91
GT-PA-03	10.81±0.12	0.56±0.01	N.D.	37.75±0.12
GT-PA-04	6.54±0.08	0.53±0.01	N.D.	26.35±0.38
GT-PA-05	12.15±0.15	0.59±0.01	N.D.	31.40±0.17
GT-PA-06	14.48±0.18	0.60±0.01	N.D.	34.95±0.23
GT-PA-07	10.43±0.10	0.60±0.01	N.D.	29.54±0.37
GT-PA-08	13.16±0.20	0.46±0.01	15.77±0.07	10.79±0.08
GT-PA-09	8.10±0.03	0.48±0.01	N.D.	24.38±0.09
GT-PA-10	15.27±0.25	0.74±0.01	N.D.	34.22±0.22
范围Range	6.54~15.27	0.46~0.74	0~15.77	10.79~37.75
平均值Mean	11.20	0.56	1.58	28.53
GT-PZ-01	6.86±0.15	0.39±0.01	23.47±0.21	2.05±0.05
GT-PZ-02	10.98±0.10	0.61±0.01	26.05±0.32	4.50±0.13
GT-PZ-03	8.77±0.01	0.58±0.01	20.78±0.17	1.59±0.02
GT-PZ-04	5.60±0.02	0.42±.001	22.97±0.34	0.88±0.02
GT-PZ-05	6.38±0.08	0.34±0.01	20.08±0.11	1.31±0.03
GT-PZ-06	8.95±0.08	0.47±0.01	22.20±0.01	2.24±0.06
GT-PZ-07	5.59±0.01	0.42±0.01	20.91±0.07	0.90±0.01
GT-PZ-08	4.40±0.02	0.34±0.01	23.23±0.32	0.66±0.01
GT-PZ-09	7.02±0.01	0.47±0.01	23.86±0.05	1.53±0.01
GT-PZ-10	11.19±0.09	0.96±0.02	24.37±0.24	4.25±0.04
GT-PZ-11	11.31±0.32	0.50±0.02	22.58±0.19	2.97±0.12
GT-PZ-12	12.83±0.21	0.60±0.01	23.31±0.17	3.66±0.06
GT-PZ-13	13.92±0.15	0.52±0.01	24.08±0.17	5.78±0.07
GT-PZ-14	11.66±0.68	0.52±0.03	22.65±1.17	4.46±0.26
GT-PZ-15	10.20±0.20	0.57±0.01	24.08±0.47	3.93±0.09
GT-PZ-16	4.91±0.19	0.36±0.01	20.73±0.68	0.86±0.04
GT-PZ-17	12.09±0.66	0.34±0.01	20.80±1.02	2.18±0.12
GT-PZ-18	9.67±0.12	0.68±0.02	27.96±0.71	2.50±0.03
GT-PZ-19	6.34±0.32	0.56±0.02	21.23±1.02	1.97±0.09
GT-PZ-20	13.01±0.16	0.79±0.03	22.51±0.06	4.69±0.04
GT-PZ-21	5.54±0.19	0.53±0.01	19.73±0.69	1.35±0.04
GT-PZ-22	4.19±0.07	0.30±0.01	20.15±0.33	0.72±0.04
GT-PZ-23	14.09±0.53	0.43±0.01	21.66±0.82	4.33±0.16
GT-PZ-24	8.52±0.03	0.42±0.01	23.30±0.06	2.35±0.01
GT-PZ-25	13.60±0.30	0.49±0.01	23.04±0.34	4.56±0.07
GT-PZ-26	14.15±0.16	0.41±0.01	23.67±0.05	3.83±0.04
GT-PZ-27	4.99±0.05	0.48±.001	24.57±0.07	4.63±0.02
GT-PZ-28	9.86±0.57	0.50±0.01	20.81±1.10	3.79±0.25
GT-PZ-29	8.79±0.04	0.80±0.01	27.47±0.28	3.93±0.06
GT-PZ-30	8.74±0.36	0.62±0.01	24.12±0.70	5.07±0.47
范围Range	4.19~14.15	0.30~0.96	19.73~27.96	0.66~5.78
平均值Mean	9.14	0.51	22.88	2.92
F值F value	0.010	2.208	2.426^**	18.735^**

材料编号	可可碱含量	茶碱含量	苦茶碱含量	咖啡碱含量
Material ID	Theobromine content	Theophylline content	Theacrine content	Caffeine content
GT-PA-01	14.18±0.62	0.48±0.01	N.D.	25.85±1.13
GT-PA-02	6.88±0.14	0.57±0.03	N.D.	30.04±0.91
GT-PA-03	10.81±0.12	0.56±0.01	N.D.	37.75±0.12
GT-PA-04	6.54±0.08	0.53±0.01	N.D.	26.35±0.38
GT-PA-05	12.15±0.15	0.59±0.01	N.D.	31.40±0.17
GT-PA-06	14.48±0.18	0.60±0.01	N.D.	34.95±0.23
GT-PA-07	10.43±0.10	0.60±0.01	N.D.	29.54±0.37
GT-PA-08	13.16±0.20	0.46±0.01	15.77±0.07	10.79±0.08
GT-PA-09	8.10±0.03	0.48±0.01	N.D.	24.38±0.09
GT-PA-10	15.27±0.25	0.74±0.01	N.D.	34.22±0.22
范围Range	6.54~15.27	0.46~0.74	0~15.77	10.79~37.75
平均值Mean	11.20	0.56	1.58	28.53
GT-PZ-01	6.86±0.15	0.39±0.01	23.47±0.21	2.05±0.05
GT-PZ-02	10.98±0.10	0.61±0.01	26.05±0.32	4.50±0.13
GT-PZ-03	8.77±0.01	0.58±0.01	20.78±0.17	1.59±0.02
GT-PZ-04	5.60±0.02	0.42±.001	22.97±0.34	0.88±0.02
GT-PZ-05	6.38±0.08	0.34±0.01	20.08±0.11	1.31±0.03
GT-PZ-06	8.95±0.08	0.47±0.01	22.20±0.01	2.24±0.06
GT-PZ-07	5.59±0.01	0.42±0.01	20.91±0.07	0.90±0.01
GT-PZ-08	4.40±0.02	0.34±0.01	23.23±0.32	0.66±0.01
GT-PZ-09	7.02±0.01	0.47±0.01	23.86±0.05	1.53±0.01
GT-PZ-10	11.19±0.09	0.96±0.02	24.37±0.24	4.25±0.04
GT-PZ-11	11.31±0.32	0.50±0.02	22.58±0.19	2.97±0.12
GT-PZ-12	12.83±0.21	0.60±0.01	23.31±0.17	3.66±0.06
GT-PZ-13	13.92±0.15	0.52±0.01	24.08±0.17	5.78±0.07
GT-PZ-14	11.66±0.68	0.52±0.03	22.65±1.17	4.46±0.26
GT-PZ-15	10.20±0.20	0.57±0.01	24.08±0.47	3.93±0.09
GT-PZ-16	4.91±0.19	0.36±0.01	20.73±0.68	0.86±0.04
GT-PZ-17	12.09±0.66	0.34±0.01	20.80±1.02	2.18±0.12
GT-PZ-18	9.67±0.12	0.68±0.02	27.96±0.71	2.50±0.03
GT-PZ-19	6.34±0.32	0.56±0.02	21.23±1.02	1.97±0.09
GT-PZ-20	13.01±0.16	0.79±0.03	22.51±0.06	4.69±0.04
GT-PZ-21	5.54±0.19	0.53±0.01	19.73±0.69	1.35±0.04
GT-PZ-22	4.19±0.07	0.30±0.01	20.15±0.33	0.72±0.04
GT-PZ-23	14.09±0.53	0.43±0.01	21.66±0.82	4.33±0.16
GT-PZ-24	8.52±0.03	0.42±0.01	23.30±0.06	2.35±0.01
GT-PZ-25	13.60±0.30	0.49±0.01	23.04±0.34	4.56±0.07
GT-PZ-26	14.15±0.16	0.41±0.01	23.67±0.05	3.83±0.04
GT-PZ-27	4.99±0.05	0.48±.001	24.57±0.07	4.63±0.02
GT-PZ-28	9.86±0.57	0.50±0.01	20.81±1.10	3.79±0.25
GT-PZ-29	8.79±0.04	0.80±0.01	27.47±0.28	3.93±0.06
GT-PZ-30	8.74±0.36	0.62±0.01	24.12±0.70	5.07±0.47
范围Range	4.19~14.15	0.30~0.96	19.73~27.96	0.66~5.78
平均值Mean	9.14	0.51	22.88	2.92
F值F value	0.010	2.208	2.426^**	18.735^**

材料编号 Material ID	没食子酸 Gallic acid	EGC	C	EC	EGCG	GCG	ECG
GT-PA-01	0.39±0.01	16.41±0.34	2.29±0.01	19.54±0.67	39.94±2.65	22.98±1.09	26.91±1.95
GT-PA-02	0.52±0.01	17.82±0.70	1.85±0.01	24.61±0.43	32.99±1.67	N.D.	28.16±1.63
GT-PA-03	0.20±0.01	N.D.	1.96±0.01	31.06±0.11	4.19±0.03	N.D.	70.87±0.47
GT-PA-04	0.19±0.01	6.99±0.25	2.06±0.01	36.35±0.44	6.34±0.06	N.D.	49.15±0.24
GT-PA-05	0.12±0.01	3.41±0.05	2.03±0.03	33.77±0.37	11.04±0.22	N.D.	71.37±2.83
GT-PA-06	0.19±0.01	N.D.	1.85±0.03	19.15±0.11	7.45±0.32	N.D.	81.30±6.95
GT-PA-07	0.33±0.01	N.D.	2.07±0.01	26.56±0.21	6.03±0.08	N.D.	74.56±1.82
GT-PA-08	0.19±0.01	N.D.	2.03±0.01	31.53±0.21	6.34±0.02	21.30±0.11	85.20±0.43
GT-PA-09	0.50±0.01	23.08±0.16	N.D.	16.94±0.03	37.05±1.41	N.D.	19.24±0.72
GT-PA-10	0.24±0.01	N.D.	1.98±0.09	25.99±0.08	6.67±0.06	N.D.	73.38±0.54
范围Range	0.12~0.52	0~23.08	0~2.29	16.94~36.35	4.19~39.94	0~22.98	19.24~85.20
平均值Mean	0.29	6.77	1.81	26.55	15.81	4.43	58.01
GT-PZ-01	0.07±0.01	2.55±0.01	2.29±0.01	47.84±0.58	6.18±0.19	N.D.	37.04±4.12
GT-PZ-02	0.17±0.01	1.88±0.04	2.70±0.01	40.74±0.22	6.36±0.10	N.D.	70.36±3.41
GT-PZ-03	0.12±0.01	3.20±0.05	2.63±0.01	42.53±0.36	7.43±0.18	N.D.	51.58±1.89
GT-PZ-04	0.08±0.01	2.82±0.08	2.19±0.04	39.14±0.73	5.51±0.01	N.D.	44.81±1.71
GT-PZ-05	0.08±0.01	1.82±0.01	2.85±0.03	46.34±0.77	5.91±0.19	N.D.	44.78±2.00
GT-PZ-06	0.09±0.01	3.04±0.01	2.63±0.01	44.64±0.21	6.90±0.12	N.D.	63.27±2.53
GT-PZ-07	0.07±0.01	2.49±0.01	2.37±0.01	51.95±0.07	6.60±0.15	N.D.	51.04±2.65
GT-PZ-08	0.07±0.01	2.89±0.01	2.14±0.01	42.91±0.42	5.64±0.06	N.D.	26.80±0.52
GT-PZ-09	0.07±0.01	2.16±0.01	2.56±0.02	48.30±0.42	6.10±0.01	N.D.	48.82±2.46
GT-PZ-10	0.17±0.01	2.39±0.05	2.31±0.01	31.20±0.34	7.65±0.08	N.D.	63.64±1.06
GT-PZ-11	0.18±0.01	1.53±0.08	2.09±0.01	24.42±0.34	6.87±0.02	N.D.	60.82±2.77
GT-PZ-12	0.16±0.01	4.27±0.05	2.35±0.04	33.78±0.19	9.11±0.03	N.D.	73.08±0.04
GT-PZ-13	0.28±0.01	1.97±0.03	2.00±0.01	20.70±0.83	8.73±0.22	N.D.	59.16±1.16
GT-PZ-14	0.14±0.01	2.20±0.10	2.54±0.06	28.28±1.10	6.32±0.07	N.D.	52.32±0.62
GT-PZ-15	0.18±0.01	3.06±0.04	2.40±0.03	25.15±0.49	7.84±0.29	N.D.	51.51±4.52
GT-PZ-16	0.07±0.01	2.14±0.08	2.75±0.09	48.15±1.87	5.78±0.11	N.D.	37.24±2.53
GT-PZ-17	0.14±0.01	3.13±0.03	2.57±0.06	46.03±2.07	7.20±0.32	N.D.	68.09±6.57
GT-PZ-18	0.11±0.01	4.27±0.42	2.00±0.01	23.53±0.68	9.41±0.42	N.D.	43.95±3.64
GT-PZ-19	0.07±0.01	5.01±0.23	2.74±0.08	51.57±3.26	5.07±0.17	N.D.	49.39±9.05
GT-PZ-20	0.17±0.01	3.16±0.14	2.34±0.01	28.24±0.12	7.33±0.06	N.D.	71.09±0.85
GT-PZ-21	0.07±0.01	1.45±0.04	2.34±0.03	40.60±1.23	5.02±0.04	N.D.	39.64±2.02
GT-PZ-22	0.04±0.01	1.56±0.06	2.02±0.01	53.81±1.44	4.84±0.01	N.D.	29.85±0.44
GT-PZ-23	0.27±0.01	2.11±0.07	2.06±0.01	24.54±0.84	9.55±0.30	N.D.	61.85±1.44
GT-PZ-24	0.09±0.01	0.92±0.01	2.59±0.01	41.23±0.24	5.40±0.06	N.D.	63.84±2.82
GT-PZ-25	0.19±0.01	2.01±0.03	2.29±0.01	23.92±0.22	6.66±0.18	N.D.	59.34±3.59
GT-PZ-26	0.17±0.01	4.56±0.02	2.12±0.01	25.98±0.13	6.53±0.05	N.D.	60.83±0.69
GT-PZ-27	0.11±0.01	3.91±0.33	2.29±0.01	37.77±0.47	6.36±0.04	N.D.	55.50±0.23
GT-PZ-28	0.17±0.01	1.55±0.01	2.25±0.05	28.51±0.72	6.25±0.01	N.D.	48.84±1.01
GT-PZ-29	0.19±.01	6.81±0.40	2.52±0.05	38.37±0.18	6.61±0.04	N.D.	83.16±1.67
GT-PZ-30	0.24±0.01	4.51±0.23	2.27±0.08	24.77±0.77	6.52±0.76	N.D.	66.16±1.68
范围Range	0.04~0.28	0.92~6.81	2.00~2.85	20.74~53.81	4.84~9.55	0	26.80~83.16
平均值Mean	0.13	2.85	2.37	36.83	6.72	0	54.59
F值F value	17.752^**	63.647	2.554^**	5.740^**	104.008	50.415	13.964

材料编号 Material ID	没食子酸 Gallic acid	EGC	C	EC	EGCG	GCG	ECG
GT-PA-01	0.39±0.01	16.41±0.34	2.29±0.01	19.54±0.67	39.94±2.65	22.98±1.09	26.91±1.95
GT-PA-02	0.52±0.01	17.82±0.70	1.85±0.01	24.61±0.43	32.99±1.67	N.D.	28.16±1.63
GT-PA-03	0.20±0.01	N.D.	1.96±0.01	31.06±0.11	4.19±0.03	N.D.	70.87±0.47
GT-PA-04	0.19±0.01	6.99±0.25	2.06±0.01	36.35±0.44	6.34±0.06	N.D.	49.15±0.24
GT-PA-05	0.12±0.01	3.41±0.05	2.03±0.03	33.77±0.37	11.04±0.22	N.D.	71.37±2.83
GT-PA-06	0.19±0.01	N.D.	1.85±0.03	19.15±0.11	7.45±0.32	N.D.	81.30±6.95
GT-PA-07	0.33±0.01	N.D.	2.07±0.01	26.56±0.21	6.03±0.08	N.D.	74.56±1.82
GT-PA-08	0.19±0.01	N.D.	2.03±0.01	31.53±0.21	6.34±0.02	21.30±0.11	85.20±0.43
GT-PA-09	0.50±0.01	23.08±0.16	N.D.	16.94±0.03	37.05±1.41	N.D.	19.24±0.72
GT-PA-10	0.24±0.01	N.D.	1.98±0.09	25.99±0.08	6.67±0.06	N.D.	73.38±0.54
范围Range	0.12~0.52	0~23.08	0~2.29	16.94~36.35	4.19~39.94	0~22.98	19.24~85.20
平均值Mean	0.29	6.77	1.81	26.55	15.81	4.43	58.01
GT-PZ-01	0.07±0.01	2.55±0.01	2.29±0.01	47.84±0.58	6.18±0.19	N.D.	37.04±4.12
GT-PZ-02	0.17±0.01	1.88±0.04	2.70±0.01	40.74±0.22	6.36±0.10	N.D.	70.36±3.41
GT-PZ-03	0.12±0.01	3.20±0.05	2.63±0.01	42.53±0.36	7.43±0.18	N.D.	51.58±1.89
GT-PZ-04	0.08±0.01	2.82±0.08	2.19±0.04	39.14±0.73	5.51±0.01	N.D.	44.81±1.71
GT-PZ-05	0.08±0.01	1.82±0.01	2.85±0.03	46.34±0.77	5.91±0.19	N.D.	44.78±2.00
GT-PZ-06	0.09±0.01	3.04±0.01	2.63±0.01	44.64±0.21	6.90±0.12	N.D.	63.27±2.53
GT-PZ-07	0.07±0.01	2.49±0.01	2.37±0.01	51.95±0.07	6.60±0.15	N.D.	51.04±2.65
GT-PZ-08	0.07±0.01	2.89±0.01	2.14±0.01	42.91±0.42	5.64±0.06	N.D.	26.80±0.52
GT-PZ-09	0.07±0.01	2.16±0.01	2.56±0.02	48.30±0.42	6.10±0.01	N.D.	48.82±2.46
GT-PZ-10	0.17±0.01	2.39±0.05	2.31±0.01	31.20±0.34	7.65±0.08	N.D.	63.64±1.06
GT-PZ-11	0.18±0.01	1.53±0.08	2.09±0.01	24.42±0.34	6.87±0.02	N.D.	60.82±2.77
GT-PZ-12	0.16±0.01	4.27±0.05	2.35±0.04	33.78±0.19	9.11±0.03	N.D.	73.08±0.04
GT-PZ-13	0.28±0.01	1.97±0.03	2.00±0.01	20.70±0.83	8.73±0.22	N.D.	59.16±1.16
GT-PZ-14	0.14±0.01	2.20±0.10	2.54±0.06	28.28±1.10	6.32±0.07	N.D.	52.32±0.62
GT-PZ-15	0.18±0.01	3.06±0.04	2.40±0.03	25.15±0.49	7.84±0.29	N.D.	51.51±4.52
GT-PZ-16	0.07±0.01	2.14±0.08	2.75±0.09	48.15±1.87	5.78±0.11	N.D.	37.24±2.53
GT-PZ-17	0.14±0.01	3.13±0.03	2.57±0.06	46.03±2.07	7.20±0.32	N.D.	68.09±6.57
GT-PZ-18	0.11±0.01	4.27±0.42	2.00±0.01	23.53±0.68	9.41±0.42	N.D.	43.95±3.64
GT-PZ-19	0.07±0.01	5.01±0.23	2.74±0.08	51.57±3.26	5.07±0.17	N.D.	49.39±9.05
GT-PZ-20	0.17±0.01	3.16±0.14	2.34±0.01	28.24±0.12	7.33±0.06	N.D.	71.09±0.85
GT-PZ-21	0.07±0.01	1.45±0.04	2.34±0.03	40.60±1.23	5.02±0.04	N.D.	39.64±2.02
GT-PZ-22	0.04±0.01	1.56±0.06	2.02±0.01	53.81±1.44	4.84±0.01	N.D.	29.85±0.44
GT-PZ-23	0.27±0.01	2.11±0.07	2.06±0.01	24.54±0.84	9.55±0.30	N.D.	61.85±1.44
GT-PZ-24	0.09±0.01	0.92±0.01	2.59±0.01	41.23±0.24	5.40±0.06	N.D.	63.84±2.82
GT-PZ-25	0.19±0.01	2.01±0.03	2.29±0.01	23.92±0.22	6.66±0.18	N.D.	59.34±3.59
GT-PZ-26	0.17±0.01	4.56±0.02	2.12±0.01	25.98±0.13	6.53±0.05	N.D.	60.83±0.69
GT-PZ-27	0.11±0.01	3.91±0.33	2.29±0.01	37.77±0.47	6.36±0.04	N.D.	55.50±0.23
GT-PZ-28	0.17±0.01	1.55±0.01	2.25±0.05	28.51±0.72	6.25±0.01	N.D.	48.84±1.01
GT-PZ-29	0.19±.01	6.81±0.40	2.52±0.05	38.37±0.18	6.61±0.04	N.D.	83.16±1.67
GT-PZ-30	0.24±0.01	4.51±0.23	2.27±0.08	24.77±0.77	6.52±0.76	N.D.	66.16±1.68
范围Range	0.04~0.28	0.92~6.81	2.00~2.85	20.74~53.81	4.84~9.55	0	26.80~83.16
平均值Mean	0.13	2.85	2.37	36.83	6.72	0	54.59
F值F value	17.752^**	63.647	2.554^**	5.740^**	104.008	50.415	13.964

成分 Component	可可碱 Theobromine	茶碱 Theophylline	苦茶碱 Theacrine	咖啡碱 Caffeine	没食子酸 Gallic acid	EGC	C	EC	EGCG	GCG
茶碱Theophylline	0.370^*
苦茶碱Theacrine	-0.200	-0.091
咖啡碱Caffeine	0.341^*	0.270	-0.953^**
没食子酸	0.382^*	0.240	-0.585^**	0.603^**
Gallic acid
EGC	-0.102	0.012	-0.383^*	0.293	0.701^**
C	-0.117	-0.034	0.525^**	-0.506^**	-0.635^**	-0.566^**
EC	-0.678^**	-0.395^*	0.369^*	-0.492^**	-0.733^**	-0.314^*	0.555^**
EGCG	0.115	0.009	-0.506^**	0.424^**	0.783^**	0.918^**	-0.556^**	-0.452^**
GCG	0.286	-0.093	-0.236	0.182	0.258	0.228	-0.034	-0.200	0.422^**
ECG	0.594^**	0.459^**	0.031	0.156	-0.086	-0.540^**	0.242	-0.168	-0.481^**	-0.007

Comparison of purine alkaloids and catechin components of wild Camellia tachangensis in Pu’an County and Panzhou County, Guizhou Province, China

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生化成分 Biochemical composition	第Ⅰ类 Group Ⅰ		第Ⅱ类 Group Ⅱ		第Ⅲ类 Group Ⅲ
生化成分 Biochemical composition	含量 Content	平均值 Average	含量 Content	平均值 Average	含量 Content	平均值 Average
可可碱Theobromine	4.19~14.15	9.14±3.20 aA	6.54~15.27	11.83±2.94 aA	6.88~15.27	12.11±4.56 aA
茶碱Theophylline	0.30~0.96	0.51±0.15 aA	0.46~0.74	0.59±0.08 aA	0.48~0.74	0.60±0.13 aA
苦茶碱Theacrine	19.73~27.96	22.88±2.04 aA	0~15.77	2.25±5.96 bB	0	0 bB
咖啡碱Caffeine	0.66~5.78	2.92±1.54 bB	10.79~37.75	29.29±8.97 aA	25.85~34.22	30.03±4.18 aA
没食子酸Gallic acid	0.04~0.28	0.13±0.06 bB	0.12~0.33	0.21±0.07 bB	0.24~0.52	0.38±0.14 aA
EGC	0.92~6.81	2.85±1.29 bB	0~6.99	1.49±2.74 bB	0~17.82	11.41±9.91 aA
C	2.00~2.85	2.37±0.24 aA	1.85~2.07	2.00±0.08 bB	1.85~2.29	2.04±0.23 bAB
EC	20.74~53.81	36.83±10.24 aA	19.15~36.35	29.20±5.76 abA	19.54~25.99	23.38±3.40 bA
EGCG	4.84~9.55	6.72±1.24 bB	4.19~11.04	6.87±2.09 bB	6.67~39.94	26.53±17.55 aA
GCG	0	0 bB	0~21.30	3.04±8.05 abAB	0~22.98	7.66±13.27 aA
ECG	26.80~83.16	54.59±13.22 bAB	49.15~85.20	72.26±11.49 aA	26.91~73.38	42.81±26.48 bB