Identification and biological characteristics of phytopathogen in Osmanthus fragrans in Zhejiang Province of China

doi:10.3969/j.issn.1004-1524.20240045

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (12): 2763-2773.DOI: 10.3969/j.issn.1004-1524.20240045

• Plant Protection • Previous Articles Next Articles

Identification and biological characteristics of phytopathogen in Osmanthus fragrans in Zhejiang Province of China

WANG Shizhen¹(), HUANG Jun², LI Mingjiang¹, HUANG Yingjie¹, ZHANG Juan¹^,^*()

1. Zhejiang Institute of Landscape Plants and Flowers, Hangzhou 311200, China
2. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2024-01-08 Online:2024-12-25 Published:2024-12-27

Abstract

Abstract:

Osmanthus fragrans, an evergreen tree with tremendous economic and ornamental value, has been extensively planted around the world. However, the disease of leaf spot seriously affected the quality and safety of Osmanthus fragrans due to uniform variety cultivation in Hangzhou. In this study, we isolated one strain that caused leaf spots and identified the fungi as Botryosphaeria dothidea by ITS sequence analysis. To clarify different factors that influence the growth of Botryosphaeria dothidea, we set different temperatures, pH values, culture media, and carbon and nitrogen sources, and the result showed its optimum temperature range was 23-28 ℃, the optimum pH value was 5, and the optimum culture medium was OMA. The growth of B. dothidea showed no obvious difference between the tested carbon and nitrogen sources. Besides, the pathogenicity was getting intense with the temperature rising and the lethal temperature was 55 ℃. The indoor antifungal test showed an inhibition rate of 20% propiconazole microemulsion was above 90%.

Key words: Botryosphaeria dothidea, leaf spot disease, biological character

CLC Number:

S436.85

WANG Shizhen, HUANG Jun, LI Mingjiang, HUANG Yingjie, ZHANG Juan. Identification and biological characteristics of phytopathogen in Osmanthus fragrans in Zhejiang Province of China[J]. Acta Agriculturae Zhejiangensis, 2024, 36(12): 2763-2773.

Figures/Tables 10

References 20

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编号 No.	供试药剂 Fungicide	母液倍数 Mother solution multiple	处理剂量(倍数) Diluted multiple	生产厂家 Manufacturer
Ⅰ	20%丙环唑微乳剂 20% Propiconazole ME	50×	10 000、15 000、 20 000、25 000、 30 000、50 000	河北中保绿农作物科技有限公司 Hebei Zhongbao Green Crop Technology Co., Ltd.
Ⅱ	咯菌腈25 g·L悬浮种衣剂 25g·L Fludioxonil FS	50×	5 000、7 500、11 250、 16 875、25 312.5、 30 000	乳山韩威生物科技有限公司 Rushan Hanwei Biotech Co.,Ltd.
Ⅲ	3%中生菌素可湿性粉剂 3% Zhongshengmycin WP	50×	5 000、7 500、11 250、 16 875、25 312.5、 30 000	河北中保绿农作物科技有限公司 Hebei Zhongbao Green Crop Technology Co., Ltd.
Ⅳ	10%苯醚甲环唑水分散粒剂 10% Difenoconazole WG	50×	5 000、7 500、11 250、 16 875、25 312.5、 30 000	先正达南通作物保护有限公司 Syngenta Nantong Crop Protection Co.,Ltd.
Ⅴ	50%咪鲜胺锰盐可湿性粉剂 50% Prochloraz-manganese chloride complex WP	50×	5 000、7 500、11 250、 16 875、25 312.5、 30 000	苏州富美实植物保护剂有限公司 FMC (Suzhou)Crop Care Co.,Ltd.

编号 No.	供试药剂 Fungicide	母液倍数 Mother solution multiple	处理剂量(倍数) Diluted multiple	生产厂家 Manufacturer
Ⅰ	20%丙环唑微乳剂 20% Propiconazole ME	50×	10 000、15 000、 20 000、25 000、 30 000、50 000	河北中保绿农作物科技有限公司 Hebei Zhongbao Green Crop Technology Co., Ltd.
Ⅱ	咯菌腈25 g·L悬浮种衣剂 25g·L Fludioxonil FS	50×	5 000、7 500、11 250、 16 875、25 312.5、 30 000	乳山韩威生物科技有限公司 Rushan Hanwei Biotech Co.,Ltd.
Ⅲ	3%中生菌素可湿性粉剂 3% Zhongshengmycin WP	50×	5 000、7 500、11 250、 16 875、25 312.5、 30 000	河北中保绿农作物科技有限公司 Hebei Zhongbao Green Crop Technology Co., Ltd.
Ⅳ	10%苯醚甲环唑水分散粒剂 10% Difenoconazole WG	50×	5 000、7 500、11 250、 16 875、25 312.5、 30 000	先正达南通作物保护有限公司 Syngenta Nantong Crop Protection Co.,Ltd.
Ⅴ	50%咪鲜胺锰盐可湿性粉剂 50% Prochloraz-manganese chloride complex WP	50×	5 000、7 500、11 250、 16 875、25 312.5、 30 000	苏州富美实植物保护剂有限公司 FMC (Suzhou)Crop Care Co.,Ltd.

药剂名称 Fungicide	稀释倍数 Dilution multiple	抑菌率 Inhibition ratio/%
10%苯醚甲环唑水分散粒剂	5 000	72 d
10% Difenoconazole WG	7 500	70 cd
	11 250	68 c
	16 875	58 b
	25 312.5	55 ab
	30 000	54 a
咯菌腈25 g·L^-1悬浮种衣剂	5 000	64 e
25g·L^-1 Fludioxonil FS	7 500	55 d
	11 250	44 c
	16 875	39 c
	25 312.5	10 a
	30 000	19 b
3%中生菌素可湿性粉剂	5 000	84 e
3% Zhongshengmycin WP	7 500	80 e
	11 250	65 d
	16 875	39 c
	25 312.5	28 b
	30 000	22 a
50%咪鲜胺锰盐可湿性粉剂	5 000	79 f
50% Prochloraz-manganese chloride	7 500	76 e
complex WP	11 250	67 d
	16 875	61 c
	25 312.5	54 b
	30 000	49 a
20%丙环唑微乳剂	10 000	91 f
20% Propiconazole ME	15 000	87 e
	20 000	83 d
	25 000	77 c
	30 000	69 b
	50 000	63 a

药剂名称 Fungicide	稀释倍数 Dilution multiple	抑菌率 Inhibition ratio/%
10%苯醚甲环唑水分散粒剂	5 000	72 d
10% Difenoconazole WG	7 500	70 cd
	11 250	68 c
	16 875	58 b
	25 312.5	55 ab
	30 000	54 a
咯菌腈25 g·L^-1悬浮种衣剂	5 000	64 e
25g·L^-1 Fludioxonil FS	7 500	55 d
	11 250	44 c
	16 875	39 c
	25 312.5	10 a
	30 000	19 b
3%中生菌素可湿性粉剂	5 000	84 e
3% Zhongshengmycin WP	7 500	80 e
	11 250	65 d
	16 875	39 c
	25 312.5	28 b
	30 000	22 a
50%咪鲜胺锰盐可湿性粉剂	5 000	79 f
50% Prochloraz-manganese chloride	7 500	76 e
complex WP	11 250	67 d
	16 875	61 c
	25 312.5	54 b
	30 000	49 a
20%丙环唑微乳剂	10 000	91 f
20% Propiconazole ME	15 000	87 e
	20 000	83 d
	25 000	77 c
	30 000	69 b
	50 000	63 a

Identification and biological characteristics of phytopathogen in Osmanthus fragrans in Zhejiang Province of China

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