Abstract:  The genetic diversity of 33 broccoli (Brassica oleracea var. italica) germplasm resources， from home and abroad， was investigated using ISSR molecular marker method. Fifteen primers were selected from 100 universal ones， and they produced 211 distinctive bands， including 180 polymorphic sites with 8531% of polymorphic bands. The average Neis genetic diversity index and Shannons information index were 0332 7 and 0487 7， respectively. The genetic distance ranged from 009 to 045 with an average value of 028. Cluster analysis divided the 33 broccoli materials into 4 groups， and showed no obvious correlation with geographic distribution. The results revealed that the ISSR markers could be used to identify broccoli germplasm resources， and the materials tested showed a certain level of genetic diversity．

Key words: Brassica oleracea var. italica, germplasm resource, ISSR, genetic diversity