Abstract: In order to clarify the effects of aquatic environment on Chinese soft\|shelled turtles （Pelodiscus sinensis）， the bacterial floras in intestines of healthy and diseased turtles and in the aquatic water were investigated. The results showed that the total bacterial numbers in intestines of healthy and diseased turtles were 32×104～713×105 cfu·g-1 and 121×105～49×107 cfu·g-1， respectively. The species of intestinal bacterial flora of diseased turtles were more complicated than the healthy ones. Enterococcus and Citrobacter were the dominant bacterial species in healthy turtles， while Citrobacte， Aeromonas and Bacillus dominated in the diseased ones. All the bacterial species isolated from aquatic water could be found in the diseased turtles， which indicated the important influence of the aquatic environment on the growth of organisms.

Key words: Pelodiscus sinensis, intestinal bacteria flora, aquatic water, isolation and identification