Abstract: The morphological structures of Empoasca onukii， the cephalic splashes， vein， abdominal apodemes， subgenital plate and the arrangement of its bristles， and the characteristics of legs were redescribed， especially the arrangement of spines were added. Ribosomal DNA （rDNA） including partial of 18S （1 843 bp）， 28S （667 bp） and complete 58S （155 bp）， ITS1 （3 114 bp）， ITS2 （1 008 bp） were isolated by PCR technology. Sequence analysis indicated that 18S， 58S and 28S had high sequence identity （90%-95%） with other insects; however， ITS1 and ITS2 showed much variability. The analysis of base composition showed that ITS1 and ITS2 were more preferences for AT （contained 660% and 651% A+T， respectively）. Comparison with the phylogenetic species revealed that ITS1 and ITS2 from E.onukii had rich polymorphic loci and ITS2 was more suitable for molecular identification of closely related species.

Key words: Empoasca onukii, morphology, rDNA, ITS2