Analysis of genetic diversity of Magnaporthe oryzae isolates from Zhejiang Province based on SSR markers#br#

Analysis of genetic diversity of Magnaporthe oryzae isolates from Zhejiang Province based on SSR markers#br#

（1 College of Chemistry and Life Science， Zhejiang Normal University， Jinhua 321004， China; 2 State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control， Institute of Plant Protection and Microbiology， Zhejiang Academy of Agricultural Sciences， Hangzhou 310021， China）

Online:2015-10-25 Published:2015-10-20

Abstract

Abstract: Twenty\|eight pairs of SSR primers that distributed on 7 chromosomes of Magnaporthe oryzae were used to study the genetic diversity of M.oryzae isolates from Zhejiang Province. M.oryzae strains isolated from 11 different areas in Zhejiang Province， from 1978 to 2011 were used as materials. Results showed that the total amplified alleles were 171， the amplified alleles of each primer pairs was 2 to 24， the average diversity index was 0534 and the average polymorphism information content was 0494. The genetic structure of M.oryzae strains isolated from different areas was different， Lishui and Hangzhou populations had the highest genetic diversity and Zhoushan population had the lowest genetic diversity. Genetic diversities of M.oryzae isolates from different years were different， the 1990 population had the highest genetic diversity, while the 1978 population had the lowest genetic diversity. The genetic structure of M.oryzae isolated from different rice types was different， a part of strains isolated from indica and japonica had the similarity genetic structure, and the strains isolated from indica rice had the higher genetic diversity than those isolated from japonica rice.

Key words: Magnaporthe oryzae, SSR, genetic structure, diversity

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Analysis of genetic diversity of Magnaporthe oryzae isolates from Zhejiang Province based on SSR markers#br#

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