Abstract:  Primer pairs were designed according to known sequences， and a nucleotide-binding site plus leucine-rich repeat （NBS-LRR） disease resistance gene， designated BoCNL1， was isolated from broccoli （Brassica oleracea var. italic）. Bioinformatic analysis were performed， and RT-PCR was used to reveal expression patterns of BoCNL1 in different organs. Results indicated that the complete coding sequence of BoCNL1 was 2 550 bp in length， encoding 849 amino acids; and the deduced protein sequence contained coiled coil(CC)， NBS， and LRR domains. Phylogenetic analysis results showed BoCNL1 was grouped with the homologous gene in B. rapa， indicating their closest relationship， and the longest genetic distance was observed between B.oleracea var. italica and Tarenaya hassleriana. RT-PCR results demonstrated that BoCNL1 expressed with low levels of transcripts in roots， flower stalks， leaves， flower buds， flowers， as well as young siliques.

Key words: Brassica oleracea var. italica, disease resistance gene, CC-NBS-LRR, gene cloning