Pathogenicity and epidemiological investigation of outbreaks of fowl adenovirus subpopulation Ⅰ infection in chickens in parts of southwestern China

doi:10.3969/j.issn.1004-1524.2017.11.06

›› 2017, Vol. 29 ›› Issue (11): 1809-1818.DOI: 10.3969/j.issn.1004-1524.2017.11.06

• Animal Scince • Previous Articles Next Articles

Pathogenicity and epidemiological investigation of outbreaks of fowl adenovirus subpopulation Ⅰ infection in chickens in parts of southwestern China

YAO Kechang, LIU Yueyue, YOU Guojin, LI Shuyun, XIA Jing, HE Xiao, LI Wenwen, DU Lijing, HAN Xinfeng, HUANG Yong^*

College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

Received:2017-05-12 Online:2017-11-20 Published:2017-12-05

Abstract

Abstract: In 2015-2016, suspected fowl adenoviruses infections were observed in many chicken farms in southwestern China. The liver samples were collected from diseased chickens and inoculated into chicken embryo kidney cells (CEK) cultures for virus isolation, and 16 fowl adenoviruses (FAdV) strains were isolated. After PCR amplificating, sequencing and comparing the conserved sequences located in the pedestal regions adjacent to the L1 loop of the hexon genes of FAdV, all of the 16 stains could be grouped into four serotypes including FAdV-4(13/16), FAdV-8a (1/16), FAdV-8 (1/16) and FAdV-2 (1/16). Then, the delegate strains from those four different serotypes were selected to infect chickens to observe its pathogenicity to chickens, the results showed that the mortality of chickens infected with CH/SCDY/1604(FAdV-4) and CH/CQBS/1504 (FAdV-8a) was 80% and 20%, respectively, while no death of chicken was observed in chickens infected by CH/GZXF/1512(FAdV-2)and CH/CQBS/1512(FAdV-8b). It was concluded that the FAdV-4 was the predominant serotype and was highly pathogenic to chickens, and hydropericardium syndrome (HPS) was the main clinical manifestation. This results could provide reference for the epidemiological investigation and prevention of FAdVⅠinfection in southwestern China.

Key words: fowl adenovirus, hydropericardium syndrome, epidemiological investigation, pathogenicity

CLC Number:

S852.65⁺7

YAO Kechang, LIU Yueyue, YOU Guojin, LI Shuyun, XIA Jing, HE Xiao, LI Wenwen, DU Lijing, HAN Xinfeng, HUANG Yong. Pathogenicity and epidemiological investigation of outbreaks of fowl adenovirus subpopulation Ⅰ infection in chickens in parts of southwestern China[J]. , 2017, 29(11): 1809-1818.

References

[1] HARRACH B, BENKÖ M, BOTH G W, et al. Family Adenoviridae [M]//KING A M Q, ADAMS M J, CARSTENS E B,et al. Virus taxonomy: classification and nomenclature of viruses. Ninth report of the international committee on taxonomy of viruses. New York: Elsevier; 2011: 125-141.
[2] MCFERRAN J B, SMYTH J A. Avian adenoviruses[J]. Revue Scientifique Et Technique , 2000,19(2):589-601.
[3] SAIF Y M, 苏敬良, 高福, 等. 禽病学[M]. 11版. 北京: 中国农业出版社,2005.
[4] HESS M. Detection and differentiation of avian adenoviruses: a review[J]. Avian Pathology , 2000, 29(3):195-206.
[5] ZHAO J, ZHONG Q, ZHAO Y, et al. Pathogenicity and complete genome characterization of fowl adenoviruses isolated from chickens associated with inclusion body hepatitis and hydropericardium syndrome in China[J]. PLoS One , 2015, 10(7):e0133073.
[6] 徐辉, LOHR J E. 检测鸡产蛋下降综合症抗体的ELISA法在田间应用的可行性研究[J]. 浙江农业学报, 1996,8(4):232-235.
XU H, LOHR J E. On study of application feasibility of the enzyme-linked immunosorbent assay for the detecting of antibody to egg drop syndrome virus in the field[J]. Acta Agriculturae Zhejiangensis , 1996,8(4):232-235.(in Chinese with English abstract)
[7] 何永强, 杜青云, 盛祖恬, 等. 一种非EDSV引起的鸭传染性减蛋症[J]. 浙江农业学报, 2001, 13(3):125-126.
HE Y Q, DU Q Y, SHENG Z T, et al. A virus different from EDSV cause duck infectious egg drop disease[J]. Acta Agriculturae Zhejiangensis , 2001, 13(3):125-126.(in Chinese with English abstract)
[8] GJEVRE A G, KALDHUSDAL M, ERIKSEN G S. Gizzard erosion and ulceration syndrome in chickens and turkeys: a review of causal or predisposing factors[J]. Avian Pathology , 2013, 42(4):297-303.
[9] CHEN H, DOU Y, ZHENG X, et al. Hydropericardium hepatitis syndrome emerged in cherry valley ducks in China[J]. Transboundary and Emerging Diseases , 2017,64(4):1262-1267.
[10] CHOI K S, KYE S J, KIM J Y, et al. Epidemiological investigation of outbreaks of fowl adenovirus infection in commercial chickens in Korea[J]. Poultry Science , 2012, 91(10):2502-2506.
[11] YE J, LIANG G, ZHANG J, et al. Outbreaks of serotype 4 fowl adenovirus with novel genotype, China[J]. Emerging Microbes & Infection , 2016 (5):e50.
[12] MATOS M, GRAFL B, LIEBHART D, et al. Selected clinical chemistry analytes correlate with the pathogenesis of inclusion body hepatitis (IBH) experimentally induced by fowl aviadenoviruses (FAdVs)[J]. Avian Pathology , 2016,45(5):520-529.
[13] JOUBERT H W, AITCHISON H, MAARTENS L H, et al. Molecular differentiation and pathogenicity of Aviadenoviruses isolated during an outbreak of inclusion body hepatitis in South Africa[J]. Journal of South African Veterinary Association , 2014, 85(1):1058.
[14] OKUDA Y, ONO M, SHIBATA I, et al. Pathogenicity of serotype 8 fowl adenovirus isolated from gizzard erosions of slaughtered broiler chickens[J]. Journal of Veterinary Medical Science , 2004, 66(12):1561-1566.
[15] MASE M, NAKAMURA K. Phylogenetic analysis of fowl adenoviruses isolated from chickens with gizzard erosion in Japan[J]. Journal of Veterinary Medical Science , 2014, 76(11):1535-1538.
[16] MENDELSON C, NOTHELFER H B, MONREAL G. Identification and characterization of an avian adenovirus isolated from a 'spiking mortality syndrome’ field outbreak in broilers on the Delmarva Peninsula, USA[J]. Avian Pathology , 1995, 24(4):693-706.
[17] DAHIYA S, SRIVASTAVA R N, HESS M, et al. Fowl adenovirus serotype 4 associated with outbreaks of infectious hydropericardium in Haryana, India[J]. Avian Diseases , 2002, 46(1):230-233.
[18] JOUBERT H W, AITCHISON H, MAARTENS L H, et al. Molecular differentiation and pathogenicity of Aviadenoviruses isolated during an outbreak of inclusion body hepatitis in South Africa[J]. Journal of the South African Veterinary Association , 2014, 85(1):1058.
[19] GOMIS S, GOODHOPE R, OJKIC D, et al. Inclusion body hepatitis as a primary disease in broilers in Saskatchewan, Canada[J]. Avian Diseases , 2006, 50(4):550-555.
[20] SAIFUDDIN M, WILKS C R. Reproduction of inclusion body hepatitis in conventionally raised chickens inoculated with a New Zealand isolate of avian adenovirus[J]. New Zealand Veterinary Journal , 1990, 38(2):62-65.
[21] KAJÁN G L, KECSKEMÉTI S, HARRACH B, et al. Molecular typing of fowl adenoviruses, isolated in Hungary recently, reveals high diversity[J]. Veterinary Microbiology , 2013, 167(3/4):357-363.
[22] NICZYPORUK J S. Phylogenetic and geographic analysis of fowl adenovirus field strains isolated from poultry in Poland[J]. Archives of Virology , 2016, 161(1):33-42.
[23] MASE M, NAKAMURA K, MINAMI F. Fowl adenoviruses isolated from chickens with inclusion body hepatitis in Japan, 2009-2010[J]. Journal of Veterinary Medical Science , 2012, 74(8):1087-1089.
[24] SCHACHNER A, MAREK A, GRAFL B, et al. Detailed molecular analyses of the hexon loop-1 and fibers of fowl aviadenoviruses reveal new insights into the antigenic relationship and confirm that specific genotypes are involved in field outbreaks of inclusion body hepatitis[J]. Veterinary Microbiology , 2016, 186: 13-20.
[25] 国纪垒, 刁有祥. Ⅰ群禽腺病毒山东株的分离鉴定及hexon基因的克隆与分析[C]//中国畜牧兽医学会2011学术年会, 成都, 2011.
[26] METTIFOGO E, NUÑEZ L F N, SANTANDER PARRA S H, et al. Fowl adenovirus Group I as a causal agent of inclusion body hepatitis/hydropericardium syndrome (IBH/HPS) outbreak in brazilian broiler flocks[J]. Pesquisa Veterin��ria Brasileira , 2014, 34(8):733-737.
[27] LIM T H, KIM B Y, KIM M S, et al. Outbreak of gizzard erosion associated with fowl adenovirus infection in Korea[J]. Poultry Science , 2012, 91(5):1113-1117.
[28] STEER P A, SANDY J R, O’ROURKE D, et al. Chronological analysis of gross and histological lesions induced by field strains of fowl adenovirus serotypes 1, 8b and 11 in one-day-old chickens[J]. Avian Pathology , 2015, 44 (2):106-113.
[29] OAKS J L, SCHRENZEL M, RIDEOUT B, et al. Isolation and epidemiology of falcon adenovirus[J]. Journal of Clinical Microbiology , 2005, 43(7):3414-3420.
[30] 刘东, 刘红祥, 于静, 等. Ⅰ亚群腺病毒在我国鸡群的流行病学调查[J]. 中国家禽, 2015, 37(15):70-73.
LIU D, LIU H X, YU J, et al. Epidemiological investigation of avian influenza adenovirus subgroup Ⅰ in China[J]. China Poultry , 2015, 37(15):70-73. (in Chinese)
[31] 李敏, 马玉峰, 杨振. Ⅰ型禽腺病毒在我国鸡群中的流行情况和防控措施[J]. 兽医导刊, 2017 (1):30-32.
LI M, MA Y F, YANG Z. Epidemic situation and control measures of avian influenza type A virus in chicken flocks in China[J]. Veterinary Orientation , 2017 (1):30-32. (in Chinese)
[32] SCHONEWILLE E, SINGH A, GÖBEL T W, et al. Fowl adenovirus (FAdV) serotype 4 causes depletion of B and T cells in lymphoid organs in specific pathogen-free chickens following experimental infection[J]. Veterinary Immunology & Immunopathology , 2008, 121(1/2):130-139.
[33] KAURI S, DEKA D, DWIVEDI P, et al. Studies on the association of hydropericardium syndrome with infectious bursal disease under field conditions[J]. Indian Journal of Poultry Science , 2011, 46(2):220-225.
[34] LEE J Y, KWON M S, CHU K S, et al. Outbreak of inclusion body hepatitis (IBH) and infectious bursal disease (IBD) in broilers, case[J]. Korean Journal of Veterinary Service , 2007, 30(3):321-327.
[35] SHAH M S, ASHRAF A, RAHMAN M, et al. A subunit vaccine against hydropericardium syndrome using adenovirus penton capsid protein[J]. Vaccine , 2012, 30(50):7153-7156.
[36] KIM M S, LIM T H, LEE D H, et al. An inactivated oil-emulsion fowl Adenovirus serotype 4 vaccine provides broad cross-protection against various serotypes of fowl Adenovirus[J]. Vaccine , 2014, 32(28):3564-3568.

Pathogenicity and epidemiological investigation of outbreaks of fowl adenovirus subpopulation Ⅰ infection in chickens in parts of southwestern China

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	XU Lihua, LAN Shengzhi, YU Bin, LI Junxing, ZHANG Pengchao, LI Baochen, SU Fei, YUAN Xiufang. Detection and genetic variation analysis of prevalent porcine circovirus type 2 strains in Zhejiang Province [J]. , 2020, 32(11): 1970-1977.
[2]	CHEN Zhang, WU Huajian, MAO Tianjiao, HAN Yeqin, SUN Pei, WEI Jianzhong, LI Dongfeng, LI Yu. Screening of strain for inactivated vaccine of Streptococcus suis type 2 [J]. , 2020, 32(1): 57-64.
[3]	ZHANG Yan, QI Yuhua, LU Yanhua, YANG Qiankun, HE Yujuan, LI Junmin, CHEN Jianping. Identification and analysis on pathogenicity-related domain of P3 protein of wheat yellow mosaic virus [J]. , 2019, 31(5): 777-783.
[4]	CAI Yue, WANG Chuyan, HUANG Minghua, CHENG Junwen, QIAN Zhongyi, GE Chunmei. Identification of Fusarium pathogens of Albizia julibrissin wilt and their cultural characteristics [J]. , 2019, 31(4): 588-599.
[5]	WU Qiongjuan, YANG Zhipeng, YAO Yanbin, LU Ping, WEI Jianzhong, SUN Pei, LI Yu. Screening of strains for inactivated vaccine of Erysipelothrix rhusiopathiae [J]. , 2018, 30(9): 1467-1475.
[6]	WANG Xin, CHENG Liang, WANG Yayi, GAO Xusheng, LI Songling. Identification of blackleg pathogen on potato (Solanum tuberosum L.) in Qinghai [J]. , 2018, 30(8): 1369-1375.
[7]	FENG Xiaoxiao, LI Haijiao, LI Ling, WANG Jiaoyu, LIN Fucheng, LU Jianping. MoSOK1, a putative germinal center kinase encoding gene, is required for fungal growth, conidiation and pathogenicity in Magnaporthe oryzae [J]. , 2018, 30(6): 999-1007.
[8]	YUAN Xuemei, YAO Jiayun, LIN Lingyun, PAN Xiaoyi, XU Yang, YIN Wenlin, SHEN Jinyu. Isolation and identification of pathogenic Aeromonas veronii from Misgurnus anguillicaudatus [J]. , 2018, 30(5): 730-737.
[9]	ZHONG Rui, JIANG Yaozhang, MA Xiaoping, ZUO Zhicai, HUANG Xiaoli, DENG Junliang, SHEN Liuhong, YU Shumin. Isolation, identification and drug sensitive test of Trichosporon loubieri from beef cattle [J]. , 2018, 30(1): 26-35.
[10]	CHEN Xianfeng, ZHANG Huili, ZHAO Lei. Isolation and identification of Xanthomonas axonopodis pv. citr on lemon samples from Taiwan [J]. , 2017, 29(1): 101-105.
[11]	ZHU Hai\\|xia， MA Yongqiang, CHENG Liang， GUO Qing\\|yun*. Pathogenicity of Alternaria tenuissima HZ-1 to broadleaf weeds and its safety on crops [J]. , 2016, 28(6): 1037-.
[12]	LI Jianqiu1， ZHOU Caiqin2,YU Bin3,WU Xuejun2， ZHAO Lingyan 2， ZHOU Lei2， ZHANG Cun3， XU Hui2，*. Seroepidemiological investigation and analysis on duck Tembusu virus antibody level in Zhejiang Province [J]. , 2016, 28(4): 563-.
[13]	DU Dan\\|chao， LU Lian\\|ming， HU Xiu\\|rong， HUANG Zhen\\|dong， ZHANG Li\\|ping， CHEN Guo\\|qing*. Isolation and identification of Purpureocillium lilacinum and its pathogenicity against Diaphorina citri [J]. , 2015, 27(3): 393-.
[14]	LIU Mao\\|xin1，2， Li Ling1，2， WANG Jiao\\|yu1,， WANG Yan\\|li1， SUN Guo\\|chang1,. The pathogenicity and infection process of Magnaporthe oryzae and Fusarium graminearum on Zizania caduciflora [J]. , 2014, 26(6): 1546-.
[15]	LUO Houqiang1， SONG Xianzhang1，*， WANG Qingyan1， DUAN Longchuan1， TU Yiqiang1， FU Hua2. Epidemiological investigation of canine parvovirus disease in small animal hospital in Wenzhou area [J]. , 2014, 26(4): 887-.