Isolation and identification of pathogenic Aeromonas veronii from Misgurnus anguillicaudatus

doi:10.3969/j.issn.1004-1524.2018.05.09

›› 2018, Vol. 30 ›› Issue (5): 730-737.DOI: 10.3969/j.issn.1004-1524.2018.05.09

• Animal Science • Previous Articles Next Articles

Isolation and identification of pathogenic Aeromonas veronii from Misgurnus anguillicaudatus

YUAN Xuemei, YAO Jiayun, LIN Lingyun, PAN Xiaoyi, XU Yang, YIN Wenlin, SHEN Jinyu^*

Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China

Received:2017-09-14 Online:2018-05-20 Published:2018-05-23

Abstract

Abstract: The present study was conducted to clarify species and drug resistance of pathogen from the diseased Misgurnus anguillicaudatus in a farm. Pathogenic bacteria from the liver of the diseased M. anguillicaudatus were examined using conventional methods and isolated. The pathogenic bacteria named strain NQ150728 were isolated and identified as Aeromonas veronii by means of VITEK-2 identification system and 16S rRNA and gyrB gene sequence analysis. The results of artificial infection test showed that the isolate could induce symptom of natural disease and caused death in 5 days. In addition, strain NQ150728 was highly sensitive to 24 kinds of drug such as ceftriaxone, riftriaxone, enrofloxacin and tetracycline.

Key words: Misgurnus anguillicaudatus, pathogenicity, Aeromonas veronii, drug sensitivity test

CLC Number:

S941.42

YUAN Xuemei, YAO Jiayun, LIN Lingyun, PAN Xiaoyi, XU Yang, YIN Wenlin, SHEN Jinyu. Isolation and identification of pathogenic Aeromonas veronii from Misgurnus anguillicaudatus[J]. , 2018, 30(5): 730-737.

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Isolation and identification of pathogenic Aeromonas veronii from Misgurnus anguillicaudatus

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