SNP screening and protein function prediction of TLR2 gene in Chinese Holstein cattle

doi:10.3969/j.issn.1004-1524.2018.08.07

›› 2018, Vol. 30 ›› Issue (8): 1321-1327.DOI: 10.3969/j.issn.1004-1524.2018.08.07

• Animal Science • Previous Articles Next Articles

SNP screening and protein function prediction of TLR2 gene in Chinese Holstein cattle

YANG Yongjiang, WU Enyun, REN Wenwen, MA Boyan, GAO Chenghong, LI Hongxu, LIU Lixia, CAO Xin, ZANG Rongxin, YANG Jutian, ZHANG Li^*

College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, China

Received:2018-01-23 Online:2018-08-25 Published:2018-08-28

Abstract

Abstract: In order to rapidly screen the Toll-like receptor 2 gene SNPs, DNA pooling and direct sequencing method were used to identify SNP mutation patterns of TLR2 gene in Chinese Holstein cattle in this study. The allele frequencies of each SNP were estimated and the effects of mutation on the TLR2 mRNA and protein were predicted by bioinformatics. The results showed that there were 6 nucleotide mutation sites in the coding region, T602A, G10900C, G11047C, A11076T, C12077T, T10634G, respectively. Among them T10634G was a missense mutation, resulting in the substitution of 64 amino acids from glutamic acid (Gly) to aspartic acid (Asp). The results can provide experimental basis on Chinese Holstein cattle breeding for disease resistance.

Key words: Chinese Holstein cattle, TLR2 gene, DNA pooling, allele frequency

CLC Number:

S823

YANG Yongjiang, WU Enyun, REN Wenwen, MA Boyan, GAO Chenghong, LI Hongxu, LIU Lixia, CAO Xin, ZANG Rongxin, YANG Jutian, ZHANG Li. SNP screening and protein function prediction of TLR2 gene in Chinese Holstein cattle[J]. , 2018, 30(8): 1321-1327.

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SNP screening and protein function prediction of TLR2 gene in Chinese Holstein cattle

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