Identification and culture conditions of a wild-type Termitomyces aurantiacus strain from Lincang

doi:10.3969/j.issn.1004-1524.2019.10.11

›› 2019, Vol. 31 ›› Issue (10): 1655-1662.DOI: 10.3969/j.issn.1004-1524.2019.10.11

• Plant Protection • Previous Articles Next Articles

Identification and culture conditions of a wild-type Termitomyces aurantiacus strain from Lincang

ZHOU Huiming^1,2, ZHANG Yanzhen^1,2, CHAI Hongmei^2,*, YANG Rongqing¹, TAN Ying¹, ZHANG Pingping¹, BAI Yuying¹, ZHAO Yilian¹, JIN Yujie¹

1. School of Biotechnology and Engineering, West Yunnan University, Lincang 677000, China;
2. Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Sciences, Kunming 650221, China

Received:2019-04-28 Online:2019-10-25 Published:2019-10-30

Abstract

Abstract: To screen the optimal culture condition for mycelial growth of Termitomyces aurantiacus and lay the foundation for its bionic cultivation, strain LCT10 was isolated from a wild mushroom fruiting body of Termitomyces from Lincang, Yunnan. Taxonomic status was identified based on its morphology and ITS sequence analysis, and the different culture conditions were designed to determine the effect of physical factors (temperature, light intensity and aeration) and chemical factors (pH, carbon resources, nitrogen sources and C/N) on mycelial growth of T. aurantiacus. The result showed that the strain LCT10 was T. aurantiacus, its morphological characteristics were consistent with its molecular identification. Its optimal temperature and pH were 25 ℃ and 8.0. It was able to grow in 5 kinds of ventilation treatments, and the growth of mycelia was the fastest in the treatment of no ventilation. Soluble starch, corn flour, sucrose, honey and glucose were suitable for its growth, honey was the best carbon soure for mycelial growth. Peptone, glycine, ammonium nitrate and urea could be used as nitrogen sources and peptone was the best. The optimum C/N for mycelial growth was (20-30)∶1. The growth of strain LCT10 under the best culture conditions had significant difference at level of 0.05 comparing with other treatments

Key words: Termitomyces aurantiacus, morphology, ITS analysis, biological characteristics

CLC Number:

S646.1⁺9

ZHOU Huiming, ZHANG Yanzhen, CHAI Hongmei, YANG Rongqing, TAN Ying, ZHANG Pingping, BAI Yuying, ZHAO Yilian, JIN Yujie. Identification and culture conditions of a wild-type Termitomyces aurantiacus strain from Lincang[J]. , 2019, 31(10): 1655-1662.

References

[1] KIRK P M, CANNON P F, DAVID J C, et al.Ainsworth & Bisby's dictionary of the fungi[M]. Wallingford: CAB1 Publishing, 2001.
[2] 周会明, 张焱珍, 张小雷, 等. 鸡枞发育生理研究进展[J]. 农业科技通讯, 2014(7): 211-214.
ZHOU H M, ZHANG Y Z, ZHANG X L, et al.Recent studies on the developmental physiology of Termitomyces aurantiacus[J]. Bulletin of Agricultural Science and Technology, 2014(7): 211-214. (in Chinese)
[3] 付子艳, 李荣春. 鸡枞菌属与华鸡枞属分类研究现状[J]. 广西植物, 2009, 29(1): 32-38.
FU Z Y, LI R C.Present research status of classification of Termitomyces and Sinotermitomyces[J]. Guihaia, 2009, 29(1): 32-38. (in Chinese with English abstract)
[4] 张玉金, 李荣春. 鸡菌与白蚁的共生专一性初步研究[J]. 食用菌学报, 2017, 24(3): 79-86.
ZHANG Y J, LI R C.On the specificity of Termitomyces-termite symbiotic interactions in Yunnan and Sichuan[J]. Acta Edulis Fungi, 2017, 24(3): 79-86. (in Chinese with English abstract)
[5] JOHNSON R A.Colony development and establishment of the fungus comb in Microtermes sp. Nr. Usambaricus(Sjostedt) (Isoptera: Macrotermitinae) from Nigeria[J]. Insectes Sociaux, 1981, 28(1): 3-12.
[6] 张玉金. 云南鸡枞菌与共生白蚁的系统发育和协同演化关系研究[D]. 昆明: 云南农业大学, 2012.
ZHANG Y J.Molecular phylogeny and coevolution between Termitomcyes and fungus-growing termites in Yunnan[D]. Kunming: Yunnan Agricultural University, 2012. (in Chinese with English abstract)
[7] 黎勇, 王小丹, 付前发, 等. 黑皮鸡枞菌的人工栽培技术研究[J]. 食用菌, 2012 (1): 37-39.
LI Y, WANG X D, FU Q F, et al.Study on artificial cultivation techniques of Termitomyces fuliginosus[J]. Edible Fungi, 2012 (1): 37-39. (in Chinese)
[8] 谭永忠, 王慧超, 戴玄, 等. 鸡枞菌/榆黄蘑的驯化栽培配方试验[J]. 北方园艺, 2015(17): 124-126.
TAN Y Z, WANG H C, DAI X, et al.Study on atificial domestication cultivation formula of Termitomyces albuminosus and Pleurotus citrinipileatus[J]. Northern Horticulture, 2015(17): 124-126.(in Chinese with English abstract)
[9] 张玉金, 郭华春, 李荣春. 鸡枞的驯化栽培现状[J]. 微生物学报, 2010, 50(10): 1288-1292.
ZHANG Y J, GUO H C, LI R C.Status of termite-mushroom artificial domestication cultivation: a review[J]. Acta Microbiologica Sinica, 2010, 50(10): 1288-1292.(in Chinese with English abstract)
[10] 王秀云. 羊肚菌生物学特性的研究[J]. 食用菌, 1998(1): 13-14.
WANG X Y.Study on the biological characteristics of Morehella esculenta[J]. Edible Fungi, 1998(1): 13-14. (in Chinese)
[11] CHEN W M, CHAI H M, ZHOU H M, et al.Phylogenetic analysis of the Agrocybe aegerita multispecies complex in Southwest China inferred from ITS and mtSSU rDNA sequences and mating tests[J]. Annals of Microbiology, 2012, 62(4): 1791-1801.
[12] 周会明, 张焱珍, 柴红梅, 等. 野生鸡?菌鉴定及最适菌株、培养基的筛选[J]. 河南农业科学, 2018, 47(7): 106-111.
ZHOU H M, ZHANG Y Z, CHAI H M, et al.Identification and screening of optimal strain and formula of wild Termitomyces[J]. Journal of Henan Agricultural Sciences, 2018, 47(7): 106-111. (in Chinese with English abstract)
[13] TAMURA K, DUDLEY J, NEI M, et al.MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0[J]. Molecular Biology and Evolution, 2007, 24(8): 1596-1599.
[14] 周会明, 张焱珍, 席亚丽, 等. 荷叶离褶伞优良菌株筛选[J]. 菌物学报, 2014, 33(2): 208-217.
ZHOU H M, ZHANG Y Z, XI Y L, et al.Screening of Lyophyllum decastes highly productive cultivable strains[J]. Mycosystema, 2014, 33(2): 208-217.(in Chinese with English abstract)
[15] 周会明, 张焱珍, 柴红梅, 等. 一株野生花脸香蘑的生物学特性及其栽培[J]. 食用菌学报, 2017, 24(1): 39-44.
ZHOU H M, ZHANG Y Z, CHAI H M, et al.Biological characteristics and cultivation of a wild-type Lepista sordida strain[J]. Acta Edulis Fungi, 2017, 24(1): 39-44. (in Chinese with English abstract)
[16] 周会明, 柴红梅, 赵静, 等. 基于SPSS的杨柳田头菇菌丝生长速度与交配型相关性分析[J]. 西南农业学报, 2010, 23(6): 1992-1998.
ZHOU H M, CHAI H M, ZHAO J, et al.Correlation analysis between mycelial growth rate and mating type of Agrocybe salicacola based on SPSS statistics[J]. Southwest China Journal of Agricultural Sciences, 2010, 23(6): 1992-1998.(in Chinese with English abstract)
[17] 冯月霞, 王琴, 吴海山, 等. 蒙古口蘑生物学特性研究[J]. 辽宁林业科技, 2013(4): 12-13.
FENG Y X, WANG Q, WU H S, et al.Biological characteristics of Tricholoma mongolium[J]. Liaoning Forestry Science and Technology, 2013(4): 12-13.(in Chinese with English abstract)
[18] 王守现, 刘宇, 许峰, 等. 荷叶离褶伞菌株的ITS鉴定及生物学特性研究[J]. 中国农学通报, 2012, 28(28): 148-152.
WANG S X, LIU Y, XU F, et al.Study on identification and biological characteristics of wild Lyophyllum decastes strain[J]. Chinese Agricultural Science Bulletin, 2012, 28(28): 148-152.(in Chinese with English abstract)
[19] 曾先富, 陈阳婷, 熊维全, 等. 鸡枞菌菌丝体培养试验[J]. 食用菌, 2012(6): 9-10.
ZENG X F, CHEN Y T, XIONG W Q, et al.The mycelium culture test of Collybia albuminosa[J]. Edible Fungi, 2012(6): 9-10. (in Chinese)
[20] 熊亚, 李敏杰, 周君. pH和温度对攀枝花野生鸡枞菌PXT-1菌株生长的影响[J]. 北方园艺, 2011(11): 164-165.
XIONG Y, LI M J, ZHOU J.Effect of pH value and temperature on growth of strain PXT-1 isolated from wild Termitomyces albuminosus in Panzhihua[J]. Northern Horticulture, 2011(11): 164-165.(in Chinese with English abstract)
[21] 胡尚勤, 刘天贵, 李贤柏. 鸡枞菌的培养条件研究[J]. 食品与生物技术学报, 2008, 27(1): 67-70.
HU S Q, LIU T G, LI X B.Research of the nutrition requirement of the Termitomyces albuminosu[J]. Journal of Food Science and Biotechnology, 2008, 27(1): 67-70.(in Chinese with English abstract)
[22] NOBRE T, KONÉ N A, KONATÉ S, et al.Dating the fungus-growing termites'mutualism shows a mixture between ancient codiversification and recent symbiont dispersal across divergent hosts[J]. Molecular Ecology, 2011, 20(12): 2619-2627.
[23] AANEN D K, EGGLETON P, ROULAND-LEFÈVRE C, et al. The evolution of fungus-growing termites and their mutualistic fungal symbionts[J]. Proceedings of the National Academy of Sciences of the United States of America, 2002, 99(23): 14887-14892.

Identification and culture conditions of a wild-type Termitomyces aurantiacus strain from Lincang

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