Molecular identification and genetic diversity of a traditional Chinese medicinal fungus Sanghuang

doi:10.3969/j.issn.1004-1524.2019.02.17

›› 2019, Vol. 31 ›› Issue (2): 307-314.DOI: 10.3969/j.issn.1004-1524.2019.02.17

• Food Science • Previous Articles Next Articles

Molecular identification and genetic diversity of a traditional Chinese medicinal fungus Sanghuang

WANG Weike¹, SONG Jiling¹, WU Youliang², YAN Jing¹, LU Na¹, YUAN Weidong¹, CHEN Guanping³

1. Institute of Vegetables, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China;
2. Jiangshan Institute of Agricultural Sciences, Jiangshan 324104, China;
3.Zhejiang Academy of Chinese Medicine, Hangzhou 310012, China

Received:2018-05-09 Online:2019-02-25 Published:2019-03-06

Abstract

Abstract: To study the genetic diversity and genetic relationship of Phellinus baumii, and their relative species by ITS molecular marker technique, and to provide the reference for Phellinus baumii identification and breeding, the ribosomal DNA internal transcribed spacer (rDNA ITS) region was amplified and sequenced to identify the 22 species of Sanghuang which were collected from domestic and detect its genetic diversity. Based on rDNA ITS sequences, the genetic distance and phylogenetic tree were obtained. Results showed that there were distinct genetic divergences among 22 species of Sanghuang, which formed three separate species cluster in the topology tree obviously with high bootstrap value. Meanwhile, two counterfeit Sanghuang strain, Fuscoporia gilva and Inonotus baumii, were detected successfully, and the certified Sanghuang germplasms were distinguished as Sanghuangporus sanghuang unambiguously by BLAST analysis. The results of ITS analysis revealed that Phellinus baumii had the plentiful genetic diversity and the genetic relationships were consistent with morphological characters of taxonomy. ITS method was efficient for identification of Phellinus baumii, which could provide a scientific basis for the resource collection and identification of the species in Phellinus baumii.

Key words: Sanghuang, ribosomal DNA internal transcribed spacer, molecular identification, genetic biodiversity analysis

CLC Number:

S567.9
R282

WANG Weike, SONG Jiling, WU Youliang, YAN Jing, LU Na, YUAN Weidong, CHEN Guanping. Molecular identification and genetic diversity of a traditional Chinese medicinal fungus Sanghuang[J]. , 2019, 31(2): 307-314.

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Molecular identification and genetic diversity of a traditional Chinese medicinal fungus Sanghuang

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