Overexpression of SgP5CS gene from Suaeda salsa enhances drought tolerance of transgenic Arabidopsis

doi:10.3969/j.issn.1004-1524.2019.04.09

›› 2019, Vol. 31 ›› Issue (4): 572-578.DOI: 10.3969/j.issn.1004-1524.2019.04.09

• Horticultural Science • Previous Articles Next Articles

Overexpression of SgP5CS gene from Suaeda salsa enhances drought tolerance of transgenic Arabidopsis

GUO Dandan¹, YANG Qinghua², ZHU Danhua², JIN Hangxia^2,*

1.College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China;
2. Institute of Crop Science and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2018-09-27 Online:2019-04-25 Published:2019-04-19

Abstract

Abstract: Δ¹-pyrroline-5-carboxylate synthetase (P5CS) is an important regulatory factor in synthesis of proline, an osmotic regulator in plants. SgP5CS gene of Suaeda salsa was cloned and introduced into Arabidopsis, the related physiological indexes and drought tolerance were determined, and the function of SgP5CS gene and regulation mechanism under drought stress were studied. The results showed that Arabidopsis with SgP5CS overexpression had long roots, while the content of free proline increased significantly and content of malondialdehyde decreased after two weeks of drought stress. It was found that overexpression of SgP5CS gene could enhance drought tolerance of transgenic Arabidopsis. These results laid a foundation to further study the SgP5CS gene function in Suaeda glauca, which might provide reference for the drought resistance mechanism in plants.

Key words: Suaeda salsa, SgP5CS gene, drought tolerance, Arabidopsis

CLC Number:

S184
Q496.1

GUO Dandan, YANG Qinghua, ZHU Danhua, JIN Hangxia. Overexpression of SgP5CS gene from Suaeda salsa enhances drought tolerance of transgenic Arabidopsis[J]. , 2019, 31(4): 572-578.

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Overexpression of SgP5CS gene from Suaeda salsa enhances drought tolerance of transgenic Arabidopsis

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