Rapid propagation system establishment of Lanzhou lily

doi:10.3969/j.issn.1004-1524.2020.06.08

›› 2020, Vol. 32 ›› Issue (6): 1000-1008.DOI: 10.3969/j.issn.1004-1524.2020.06.08

• Horticultural Science • Previous Articles Next Articles

Rapid propagation system establishment of Lanzhou lily

KE Yiqiang^1,2, GUO Penghui¹, MA Hongxin^1,2, YANG Xuhua^1,2, GAO Dandan^1,2,*, LIU Xiangjun¹, MA Zhongren², DING Gongtao²

1. College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730124, China;
2. Biomedical Research Center, Northwest Minzu University, Lanzhou 730030, China

Received:2020-01-08 Online:2020-06-25 Published:2020-06-24

Abstract

Abstract: To solve the shortage of Lanzhou lily seedlings, micro-propagation system for mass production of Lanzhou lily seedlings was discussed in this study. Fresh bulbs of Lanzhou lily were used as the initial plant material, and MS and 1/2MS medium were used as the basic medium. The orthogonal design was used to study the effects of different disinfectants on survival rate of explants, and different plant growth regulators on callus and clustered shoots induction. The response surface method also was used to reveal the effects of different hormones combinations on rooting. The results were as follows: (1) The best disinfectant combination was 75% alcohol disinfection for 20 s+2% sodium hypochlorite solution for 10 min. The survival rate of explant was 96.30%. (2) The best medium formulation for callus induction was MS + 0.3 mg·L^-1 NAA + 3 mg·L^-1 6-BA, and the induction rate could reach 96.33%. (3) The best medium formula to promote clustered shoots induction was MS + 0.3 mg·L^-1 NAA +3 mg·L^-1 6-BA, and the induction rate could reach 98.00%. (4) The best medium formula for rooting was 1/2 MS + 0.5 mg·L^-1 NAA + 3 mg·L^-1 6-BA + 2 mg·L^-1 activated carbon, the rooting rate could reach 94.00%.

Key words: Lanzhou lily, rapid propagation system, plant tissue culture, response surface method

CLC Number:

KE Yiqiang, GUO Penghui, MA Hongxin, YANG Xuhua, GAO Dandan, LIU Xiangjun, MA Zhongren, DING Gongtao. Rapid propagation system establishment of Lanzhou lily[J]. , 2020, 32(6): 1000-1008.

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Rapid propagation system establishment of Lanzhou lily

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