Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (3): 590-598.DOI: 10.3969/j.issn.1004-1524.2022.03.20
• Biosystems Engineening • Previous Articles Next Articles
YAN Ning1,2(
), ZHANG Han2,*(
), DONG Hongtu3, KANG Kai3, LUO Bin2
Received:2021-07-19
Online:2022-03-25
Published:2022-03-30
Contact:
ZHANG Han
CLC Number:
YAN Ning, ZHANG Han, DONG Hongtu, KANG Kai, LUO Bin. Wheat variety recognition method based on same position segmentation of transmitted light and reflected light images[J]. Acta Agriculturae Zhejiangensis, 2022, 34(3): 590-598.
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URL: http://www.zjnyxb.cn/EN/10.3969/j.issn.1004-1524.2022.03.20
Fig.1 Purity detection device 1, Darkroom; 2, Industrial camera; 3, Microscope; 4, Active ring light source; 5, Back light source plate; 6, Seed samples; 7, Stage; 8, Wheat seeds; 9, Computer.
Fig.5 Morphological treatment of embryonic region a, Embryo binarization; b, Split connected domain; c, Embryo contour selection; d, Region closed operation.
Fig.6 Images of embryo and endosperm sections a, Reflected light image of embryo; b, Transmitted light image of embryo; c, Reflected light image of endosperm; d, Transmitted light image of endosperm.
| 特征分类 Feature classification | 提取特征 Extract features | 分选区间 Sorting interval | 京麦9 Jingmai 9 | 京麦11 Jingmai 11 | 济麦22 Jimai 22 | 济麦44 Jimai 44 | 准确率 Accuracy/% |
|---|---|---|---|---|---|---|---|
| 整粒Seeds | Btm | <84.215 | 3 | 4 | 49 | 47 | 94.5 |
| ≥84.215 | 47 | 46 | 1 | 3 | |||
| Rtd | <35.09 | 2 | 4 | 49 | 43 | 93.0 | |
| ≥35.09 | 48 | 46 | 1 | 7 | |||
| Gtd | <33.395 | 4 | 4 | 49 | 43 | 92.0 | |
| ≥33.395 | 46 | 46 | 1 | 7 | |||
| Vtd | <35.22 | 2 | 4 | 49 | 43 | 93.0 | |
| ≥35.22 | 48 | 46 | 1 | 7 | |||
| 胚部Seed embryo | Btm | <59.365 | 3 | 2 | 46 | 44 | 92.5 |
| ≥59.365 | 47 | 48 | 4 | 6 | |||
| 胚乳部Seed endosperm | Btm | <89.78 | 4 | 3 | 49 | 46 | 94.0 |
| ≥89.78 | 46 | 47 | 1 | 4 | |||
| Rtd | <30.405 | 5 | 5 | 48 | 43 | 90.5 | |
| ≥30.405 | 45 | 45 | 2 | 7 | |||
| Vtd | <30.52 | 5 | 5 | 48 | 43 | 90.5 | |
| ≥30.52 | 45 | 45 | 2 | 7 |
Table 1 Identification results based on threshold interval according to a single feature
| 特征分类 Feature classification | 提取特征 Extract features | 分选区间 Sorting interval | 京麦9 Jingmai 9 | 京麦11 Jingmai 11 | 济麦22 Jimai 22 | 济麦44 Jimai 44 | 准确率 Accuracy/% |
|---|---|---|---|---|---|---|---|
| 整粒Seeds | Btm | <84.215 | 3 | 4 | 49 | 47 | 94.5 |
| ≥84.215 | 47 | 46 | 1 | 3 | |||
| Rtd | <35.09 | 2 | 4 | 49 | 43 | 93.0 | |
| ≥35.09 | 48 | 46 | 1 | 7 | |||
| Gtd | <33.395 | 4 | 4 | 49 | 43 | 92.0 | |
| ≥33.395 | 46 | 46 | 1 | 7 | |||
| Vtd | <35.22 | 2 | 4 | 49 | 43 | 93.0 | |
| ≥35.22 | 48 | 46 | 1 | 7 | |||
| 胚部Seed embryo | Btm | <59.365 | 3 | 2 | 46 | 44 | 92.5 |
| ≥59.365 | 47 | 48 | 4 | 6 | |||
| 胚乳部Seed endosperm | Btm | <89.78 | 4 | 3 | 49 | 46 | 94.0 |
| ≥89.78 | 46 | 47 | 1 | 4 | |||
| Rtd | <30.405 | 5 | 5 | 48 | 43 | 90.5 | |
| ≥30.405 | 45 | 45 | 2 | 7 | |||
| Vtd | <30.52 | 5 | 5 | 48 | 43 | 90.5 | |
| ≥30.52 | 45 | 45 | 2 | 7 |
| 二分类小麦品种 Binary classification of wheat varieties | 整粒透射光 Seeds transmitted light | 整粒反射光 Seeds reflected light | 整粒全光 Seeds full light | 分割透射光 Transmitted light after seeds segmentation | 分割反射光 Reflected light after seeds segmentation | 分割全光 Full light after seeds segmentation | 全部特征 All features |
|---|---|---|---|---|---|---|---|
| 京麦9、济麦22 | 98.88 | 95.50 | 99.43 | 99.43 | 96.38 | 99.60 | 99.98 |
| Jingmai 9、Jimai 22 | |||||||
| 京麦9、济麦44 | 98.90 | 95.55 | 99.10 | 99.45 | 97.63 | 99.53 | 99.90 |
| Jingmai 9、Jimai 44 | |||||||
| 京麦11、济麦22 | 98.68 | 95.25 | 99.00 | 99.10 | 95.18 | 99.18 | 99.58 |
| Jingmai 11、Jimai 22 | |||||||
| 京麦11、济麦44 | 98.43 | 95.08 | 99.10 | 99.33 | 94.53 | 99.60 | 99.68 |
| Jingmai 11、Jimai 44 | |||||||
| 京麦组、济麦组 | 99.38 | 95.78 | 99.69 | 99.61 | 97.58 | 99.78 | 99.84 |
| Jingmai Group、Jimai Group | |||||||
| 济麦22、济麦44 | 69.48 | 73.28 | 83.00 | 81.60 | 76.88 | 83.75 | 84.60 |
| Jimai 22、Jimai 44 | |||||||
| 京麦9、京麦11 | 59.98 | 74.15 | 75.28 | 72.45 | 76.20 | 81.83 | 83.73 |
| Jingmai 9、Jingmai 11 |
Table 2 Classification accuracy of PLS-DA seeds analysis %
| 二分类小麦品种 Binary classification of wheat varieties | 整粒透射光 Seeds transmitted light | 整粒反射光 Seeds reflected light | 整粒全光 Seeds full light | 分割透射光 Transmitted light after seeds segmentation | 分割反射光 Reflected light after seeds segmentation | 分割全光 Full light after seeds segmentation | 全部特征 All features |
|---|---|---|---|---|---|---|---|
| 京麦9、济麦22 | 98.88 | 95.50 | 99.43 | 99.43 | 96.38 | 99.60 | 99.98 |
| Jingmai 9、Jimai 22 | |||||||
| 京麦9、济麦44 | 98.90 | 95.55 | 99.10 | 99.45 | 97.63 | 99.53 | 99.90 |
| Jingmai 9、Jimai 44 | |||||||
| 京麦11、济麦22 | 98.68 | 95.25 | 99.00 | 99.10 | 95.18 | 99.18 | 99.58 |
| Jingmai 11、Jimai 22 | |||||||
| 京麦11、济麦44 | 98.43 | 95.08 | 99.10 | 99.33 | 94.53 | 99.60 | 99.68 |
| Jingmai 11、Jimai 44 | |||||||
| 京麦组、济麦组 | 99.38 | 95.78 | 99.69 | 99.61 | 97.58 | 99.78 | 99.84 |
| Jingmai Group、Jimai Group | |||||||
| 济麦22、济麦44 | 69.48 | 73.28 | 83.00 | 81.60 | 76.88 | 83.75 | 84.60 |
| Jimai 22、Jimai 44 | |||||||
| 京麦9、京麦11 | 59.98 | 74.15 | 75.28 | 72.45 | 76.20 | 81.83 | 83.73 |
| Jingmai 9、Jingmai 11 |
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