Genome-wide identification and bioinformatics analysis of BES1 transcription factor family in mango

doi:10.3969/j.issn.1004-1524.2022.05.13

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (5): 984-994.DOI: 10.3969/j.issn.1004-1524.2022.05.13

• Horticultural Science • Previous Articles Next Articles

Genome-wide identification and bioinformatics analysis of BES1 transcription factor family in mango

XIA Yuqi¹^,²(), SUN Yu¹^,², LIU Zhixin², SUN Ruiqing¹^,², YANG Nan¹^,², PU Jinji²^,³, ZHANG He¹^,²^,³^,^*()

1. College of Plant Protection, College of Tropical Crops, Hainan University, Haikou 570228, China
2. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture and Rural Affairs, Haikou 571101, China
3. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China

Received:2021-03-15 Online:2022-05-25 Published:2022-06-06
Contact: ZHANG He

Abstract

Abstract:

BES1/BZR1 is a unique transcription factor in the plant and the only transcription factor in the brassinolide (BR) signal transduction pathway. To explore the role BES1 plays on the stress resistance and diseases resistance in mango, we used bioinformatics methods to analyze the physicochemical properties, domains, protein structures and gene expression of BES1s family in mango. The results showed that in mango that the number of exons varies greatly, and all BES1s family members have the BES1_N domain. Evolution analysis and protein secondary structures showed that three groups were divided in BES1s family of mango, and there were significant differences among different groups. The qRT-PCR assay’s results showed that during the infection process of Cg, MiBES1.1 and MiBES1.5 were continuously up-regulated expression, and MiBES1.12 and MiBES1.13 lasted down-regulated expression; during the infection process of Xcm, mango’s BES1s family up-regulated expression at 12 h, except MiBES1.7 and MiBES1.9, and MiBES1.1~MiBES1.5 and MiBES1.11 down-regulated expression at 3 h; during the MeJA treatment process, MiBES1.7、MiBES1.9 and MiBES1.2 lasted down-regulation at 48 h, mango’s BES1s gene family down-regulated expression, except MiBES1.5 and MiBES1.11~MiBES1.13. A scientific basis is provided for the further study of the BES1s family function in mango.

Key words: transcription factor, mango, bioinformatics

CLC Number:

S667.7

XIA Yuqi, SUN Yu, LIU Zhixin, SUN Ruiqing, YANG Nan, PU Jinji, ZHANG He. Genome-wide identification and bioinformatics analysis of BES1 transcription factor family in mango[J]. Acta Agriculturae Zhejiangensis, 2022, 34(5): 984-994.

Figures/Tables 9

References 38

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基因 Gene	氨基酸数 Number of amino acids/aa	蛋白质分子量 MW/ku	蛋白等电点 pI	亲水性平均系数 GRAVY	脂溶指数 Aliphatic index	不稳定指数 Instability index	外显子 Exon
MiBES1.1	629	71.63	5.98	-0.400	72.23	38.79	2
MiBES1.2	722	81.87	6.12	-0.502	70.11	44.91	11
MiBES1.3	697	77.81	5.60	-0.411	74.40	46.53	11
MiBES1.4	697	77.94	5.66	-0.414	74.68	46.41	10
MiBES1.5	303	33.38	9.06	-0.624	62.18	65.57	10
MiBES1.6	303	33.42	9.20	-0.669	62.18	66.06	2
MiBES1.7	326	35.26	8.38	-0.588	57.24	62.79	2
MiBES1.8	1 278	142.67	7.57	-0.434	78.42	47.88	21
MiBES1.9	321	34.63	8.62	-0.551	62.02	67.39	2
MiBES1.10	441	47.89	8.70	-0.375	64.85	55.19	6
MiBES1.11	322	34.83	8.86	-0.570	63.70	66.21	2
MiBES1.12	345	36.84	7.98	-0.482	59.19	66.70	4
MiBES1.13	652	73.06	6.59	-0.337	76.41	41.30	9

基因 Gene	氨基酸数 Number of amino acids/aa	蛋白质分子量 MW/ku	蛋白等电点 pI	亲水性平均系数 GRAVY	脂溶指数 Aliphatic index	不稳定指数 Instability index	外显子 Exon
MiBES1.1	629	71.63	5.98	-0.400	72.23	38.79	2
MiBES1.2	722	81.87	6.12	-0.502	70.11	44.91	11
MiBES1.3	697	77.81	5.60	-0.411	74.40	46.53	11
MiBES1.4	697	77.94	5.66	-0.414	74.68	46.41	10
MiBES1.5	303	33.38	9.06	-0.624	62.18	65.57	10
MiBES1.6	303	33.42	9.20	-0.669	62.18	66.06	2
MiBES1.7	326	35.26	8.38	-0.588	57.24	62.79	2
MiBES1.8	1 278	142.67	7.57	-0.434	78.42	47.88	21
MiBES1.9	321	34.63	8.62	-0.551	62.02	67.39	2
MiBES1.10	441	47.89	8.70	-0.375	64.85	55.19	6
MiBES1.11	322	34.83	8.86	-0.570	63.70	66.21	2
MiBES1.12	345	36.84	7.98	-0.482	59.19	66.70	4
MiBES1.13	652	73.06	6.59	-0.337	76.41	41.30	9

基因 Gene	正向引物 Forward primer(5'→3')	反向引物 Reverse primer (5' →3')
MiBES1.1	GATGACTTGTTTGTGGCGGG	ATGTCCCCGCAATCTAGCAG
MiBES1.2	AGGATGGGATGGAGGTATCC	CCCTCGTTCACAGAAGAAGC
MiBES1.3	GCTGAATGCTGCATGGGATG	CAACAACTGCTTCCCCATGC
MiBES1.4	TCCTGATGGAACCACCTTTC	GCTGAAGATTGGGACTGAGC
MiBES1.5	AGCACCTCAACCAGGATCAC	ATCCGGAGAGGAGGAAGAGA
MiBES1.6	TTTTCCACTCCTCCGAAATG	CAACTGACGATGCATTGGAC
MiBES1.7	AAGAGACGAGAGCGCAGAAG	TAGCTGATGCAGACCCTCCT
MiBES1.8	CTACACAACGATGGGCAATG	TAGCTGACTGAGGCCTGGTT
MiBES1.9	TCTTCATTTCCAAGCCCAAC	TTACCCAACGACCAGAGTCC
MiBES1.10	AGGAAGAAGTTATAGCTGGTGGTG	ACATTCTTCATGAATCCTCTCTCC
MiBES1.11	CCACCTCTATCATCTCCAACTTCT	ATCACATTCTGGTATTGTGACAGG
MiBES1.12	TTAGAGATGAGGTTCTGTCAGGTG	AACTGGTTCTAGAGTTTCCCAGTG
MiBES1.13	TGTAGACCTTTGGAAGAGAAGGAG	CAGGAGAGATTCCTCTTTGAGAAG
Miactin	GGCAAGTCTGGTGCCAG	ACGGTATCTATCTCTTCG

基因 Gene	正向引物 Forward primer(5'→3')	反向引物 Reverse primer (5' →3')
MiBES1.1	GATGACTTGTTTGTGGCGGG	ATGTCCCCGCAATCTAGCAG
MiBES1.2	AGGATGGGATGGAGGTATCC	CCCTCGTTCACAGAAGAAGC
MiBES1.3	GCTGAATGCTGCATGGGATG	CAACAACTGCTTCCCCATGC
MiBES1.4	TCCTGATGGAACCACCTTTC	GCTGAAGATTGGGACTGAGC
MiBES1.5	AGCACCTCAACCAGGATCAC	ATCCGGAGAGGAGGAAGAGA
MiBES1.6	TTTTCCACTCCTCCGAAATG	CAACTGACGATGCATTGGAC
MiBES1.7	AAGAGACGAGAGCGCAGAAG	TAGCTGATGCAGACCCTCCT
MiBES1.8	CTACACAACGATGGGCAATG	TAGCTGACTGAGGCCTGGTT
MiBES1.9	TCTTCATTTCCAAGCCCAAC	TTACCCAACGACCAGAGTCC
MiBES1.10	AGGAAGAAGTTATAGCTGGTGGTG	ACATTCTTCATGAATCCTCTCTCC
MiBES1.11	CCACCTCTATCATCTCCAACTTCT	ATCACATTCTGGTATTGTGACAGG
MiBES1.12	TTAGAGATGAGGTTCTGTCAGGTG	AACTGGTTCTAGAGTTTCCCAGTG
MiBES1.13	TGTAGACCTTTGGAAGAGAAGGAG	CAGGAGAGATTCCTCTTTGAGAAG
Miactin	GGCAAGTCTGGTGCCAG	ACGGTATCTATCTCTTCG

Genome-wide identification and bioinformatics analysis of BES1 transcription factor family in mango

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