Identification and main biological characteristics of pathogen of Clematis leaf blight in Yunnan

doi:10.3969/j.issn.1004-1524.2022.07.12

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (7): 1449-1456.DOI: 10.3969/j.issn.1004-1524.2022.07.12

• Plant protection • Previous Articles Next Articles

Identification and main biological characteristics of pathogen of Clematis leaf blight in Yunnan

YANG Ling¹^,²(), SHA Nanjing², PAN Pengju², WU Bozhi¹^,^*()

1. College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201,China
2. Key Laboratory for Forest Biotechnology in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224,China

Received:2021-02-23 Online:2022-07-25 Published:2022-07-26
Contact: WU Bozhi

Abstract

Abstract:

In order to identify the pathogen of Clematis leaf blight in Yunnan Province, the typical diseased leaves were collected from the diseased plants. The pathogen was isolated and purified by tissue separation method, and identified by morphological characteristics combined with sequence analysis of ribosomal internal transcribed spacer (ITS) and large subunit (LSU), and verified by Koch postulates. The main biological characteristics of the pathogen were determined with different media, temperature, light, pH, carbon and nitrogen sources. The results showed that the pathogen of Clematis leaf blight was Spencermartinia sp., according to Koch postulates, all the leaves inoculated with the pathogen were infected. Potato dextrose agar (PDA) and potato saccharose agar(PSA) were the suitable media, the optimum temperature for growth was 25 ℃, the growth was not affected under different light treatments, the optimum pH for growth was 6.5-7.5, the best carbon source was glucose and sucrose, and the best nitrogen source was sodium nitrate.

Key words: Clematis leaf blight, pathogen identification, biological characteristics

CLC Number:

S435.67

YANG Ling, SHA Nanjing, PAN Pengju, WU Bozhi. Identification and main biological characteristics of pathogen of Clematis leaf blight in Yunnan[J]. Acta Agriculturae Zhejiangensis, 2022, 34(7): 1449-1456.

Figures/Tables 7

Fig.1 Comparison of diseased and inoculated plants of Clematis A, Symptoms of naturally diseased plant; B, Symtoms of the inoculated plant; C, Control.

Fig.2 Morphological characteristics of the pathogen A, The front of colony on the 5th day; B, The back of colony on the 5th day; C, Loose and aggregated conidiomata; D,Pycnidia; E, Conidia; F, Conidiogenous cells and young conidia.

Fig.3 Phylogram generated from maximum likeihood analysis based on combined ITS and LSU Type species were given in bold typeface;★ represented data from the study; In the figure, the value before the slash represented the support value, and the value after the slash represented the ultrafast bootstrap value.

Fig.4 Effect of different media on the pathogen growth Different lowercase letters represent significant difference at 0.05 level. The same as below.

Fig.5 Effect of different temperatures on the pathogen growth

Fig.6 Effect of different pH values on the pathogen growth

Fig.7 Effect of carbon source and nitrogen source on the pathogen growth A, Effect of carbon source on the pathogen growth; B, Effect of nitrogen source on the pathogen growth.

References 16

[1]	李志坚, 管开云, 李景秀, 等. 铁线莲属植物上的病虫害及防治[J]. 植物保护, 2002, 28(2): 35-36.
	LI Z J, GUAN K Y, LI J X, et al. Diseases and pests of cultivated Clematis and their control[J]. Plant Protection, 2002, 28(2): 35-36. (in Chinese with English abstract)
[2]	GOLZAR H, WANG C, WILLYAMS D. First report of Phoma clematidina the cause of leaf spot-wilt disease of Clematis pubescens in Australia[J]. Australasian Plant Disease Notes, 2011, 6(1): 87-90. DOI URL
[3]	李同水. 藤本皇后铁线莲[M]. 长春: 吉林科学技术出版社, 2012.
[4]	新锐园艺工作室组. 铁线莲初学者手册[M]. 2版. 北京: 中国农业出版社, 2019.
[5]	方中达. 植病研究方法[M]. 3版. 北京: 中国农业出版社, 1998.
[6]	ZHANG D, GAO F L, JAKOVLIĆ I, et al. PhyloSuite: an integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies[J]. Molecular Ecology Resources, 2020, 20(1): 348-355. DOI URL
[7]	DISSANAYAKE A J. Botryosphaeriaceae: current status of genera and species[J]. Mycosphere, 2016, 7(7): 1001-1073. DOI URL
[8]	PHILLIPS A J L, ALVES A, ABDOLLAHZADEH J, et al. The Botryosphaeriaceae: genera and species known from culture[J]. Studies in Mycology, 2013, 76: 51-167. DOI URL
[9]	ABDOLLAHZADEH J, JAVADI A, ZARE R, et al. A phylogenetic study of Dothiorella and Spencermartinsia species associated with woody plants in Iran, New Zealand, Portugal and Spain[J]. Persoonia-Molecular Phylogeny and Evolution of Fungi, 2014, 32(1): 1-12. DOI URL
[10]	ZHANG M. Two new species of Spencermartinsia(Botryosphaeriaceae, botryosphaeriales) from China[J]. Mycosphere, 2016, 7(7): 942-949. DOI URL
[11]	ADESEMOYE A O, ESKALEN A. First report of Spencermartinsia viticola, Neofusicoccum australe, and N. parvum causing branch canker of Citrus in California[J]. Plant Disease, 2011, 95(6): 770.
[12]	JAMI F, SLIPPERS B, WINGFIELD M J, et al. Greater Botryosphaeriaceae diversity in healthy than associated diseased Acacia karroo tree tissues[J]. Australasian Plant Pathology, 2013, 42(4): 421-430. DOI URL
[13]	HASHEMI H, MOHAMMADI H, ABDOLLAHZADEH J. Symptoms and fungi associated with elm trees decline in Iran[J]. European Journal of Forest Research, 2017, 136(5/6): 857-879. DOI URL
[14]	GONZÁLEZ-DOMÍNGUEZ E, ALVES A, LEÓN M, et al. Characterization of Botryosphaeriaceae species associated with diseased loquat (Eriobotrya japonica) in Spain[J]. Plant Pathology, 2017, 66(1): 77-89. DOI URL
[15]	叶清桐, 李亚萌, 周悦妍, 等. 国内外葡萄枝干病害的发生危害与病原菌种类[J]. 果树学报, 2021, 38(2): 278-292.
	YE Q T, LI Y M, ZHOU Y Y, et al. Occurrence of grapevine trunk diseases caused by fungal pathogens in the domestic and overseas[J]. Journal of Fruit Science, 2021, 38(2): 278-292. (in Chinese with English abstract)
[16]	郭徐鹏. 毛白杨叶际微生物多样性及对杨树溃疡病生物防治研究[D]. 杨凌: 西北农林科技大学, 2009.
	GUO X P. Diversity of phyllosphere of Populus tomentosa and control effect against canker of poplar[D]. Yangling: Northwest A & F University, 2009. (in Chinese with English abstract)

Identification and main biological characteristics of pathogen of Clematis leaf blight in Yunnan

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References 16

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SHEN Guoqiang, WANG Mingde, GUAN Yawen, WU Jun, WANG Hanrong. Identification, biological characteristics and control agents screening of pathogens of curvular leaf spot disease of sorghum [J]. Acta Agriculturae Zhejiangensis, 2025, 37(7): 1492-1500.
[2]	LIU Pengfei, ZHANG Shuhan, HONG Kai, SHAO Yue, LOU Binggan. Isolation and identification of the pathogen causing tomato canker in Zhejiang Province of China [J]. Acta Agriculturae Zhejiangensis, 2025, 37(6): 1293-1300.
[3]	WANG Chao, LI Yanjie, NIU Yun, WEN Lianhao, CHEN Jingjing, WU Hongzhi, YANG Yuyong, WU Yandi. Biological characterization of black spot pathogens in Rosa chinensis and evaluation of varietal resistance [J]. Acta Agriculturae Zhejiangensis, 2025, 37(5): 1087-1096.
[4]	JI Mengting, CHEN Changjiang, ZHU Ling, ZHAN Menglin, XIAO Shun, CAI Xueqing. Identification of etiological bacterium causing leaf spot disease on Ficus carica [J]. Acta Agriculturae Zhejiangensis, 2025, 37(5): 1097-1106.
[5]	LI Yani, CHEN Weiliang, MAO Bizeng. Pathogen identification of root rot of Curcuma wenyujin [J]. Acta Agriculturae Zhejiangensis, 2024, 36(5): 1086-1093.
[6]	LUO Zhihan, LIU Pengfei, YU Jun, QI He, CHEN Xiaoguang, LOU Binggan. Identification and biological characteristics of pathogen causing branch dieback on Styphnolobium japonicum (L.) Schott [J]. Acta Agriculturae Zhejiangensis, 2024, 36(3): 579-588.
[7]	SUN Shanshan, CHEMI Lhamo, LI Qiang, ZENG Nanfang, ZHENG Cheng, ZHANG Baiyu, YAN Qigui. Construction and biological characteristics of a recombinant pseudorabies virus expressing GP5-M of PRRSV NADC30-like virus strain [J]. Acta Agriculturae Zhejiangensis, 2023, 35(11): 2555-2567.
[8]	WANG Zhipeng, ZHAO Jian, HUANG Pan, CUI Xuemei, NAN Li, SONG Houhui, BAO Guolian, LIU Yan. Isolation, identification and biological characteristics of rabbit-derived Escherichia coli bacteriophage [J]. Acta Agriculturae Zhejiangensis, 2022, 34(8): 1599-1608.
[9]	LIU Na, FAN Qiaochu, ZHOU Jia, SONG Yajing, ZHANG Guwen, FENG Zhijuan, BO Yuanpeng, WANG Bin, GONG Yaming. Identification and control of anthracnose in vegetable soybean [J]. Acta Agriculturae Zhejiangensis, 2022, 34(12): 2682-2688.
[10]	CHEN Runchen, WANG Yining, LIU Xiaowen, WANG Hongyan, DING Qiang, WANG Honglei. Identification, artificial cultivation and nutritional analysis of wild Pholiota adiposa [J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2330-2338.
[11]	LI Xuqing, YAN Jianli, RUAN Songlin. Identification and biological characteristics of anthracnose pathogen on Tetrastigma hemsleyanum [J]. , 2020, 32(11): 2009-2019.
[12]	LI Xuqing, ZHANG Jingze, ZHANG Ya, WU Genliang. Identification of pathotype of Verticillium dahlida isolates on eggplant and their biological characteristics in Shaoxing City, Zhejiang Province [J]. , 2019, 31(5): 784-789.
[13]	ZHOU Huiming, ZHANG Yanzhen, CHAI Hongmei, YANG Rongqing, TAN Ying, ZHANG Pingping, BAI Yuying, ZHAO Yilian, JIN Yujie. Identification and culture conditions of a wild-type Termitomyces aurantiacus strain from Lincang [J]. , 2019, 31(10): 1655-1662.
[14]	MA Xiaoping, YANG Qiuxia, YU Yan, LI Desheng, WANG Chengdong, LING Shanshan, GU Yu. Comparison of partial biological characteristics and drug sensitivity between Cladosporium cladosporioides wild strain (Z20) from giant panda and mutant strain (Zt) [J]. , 2018, 30(8): 1328-1335.
[15]	LEI Xueping, GENG Yi, YU Zehui, ZHENG Liping, CAO Shiqi, HUANG Xiaoli, CHEN Defang, OUYANG Ping, LIU Kairui. Isolation and identification of Elizabethkingia meningoseptica from Chinese spiny frog (Quasipaa spinosa) and pathological lesions of its infection [J]. , 2018, 30(3): 371-377.