Phyllosphere microecology of brown spot and healthy tobacco leaves after application of myclobutanil

doi:10.3969/j.issn.1004-1524.20230242

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (1): 156-167.DOI: 10.3969/j.issn.1004-1524.20230242

• Plant Protection • Previous Articles Next Articles

Phyllosphere microecology of brown spot and healthy tobacco leaves after application of myclobutanil

ZHANG Yi¹^,²(), WANG Feng²^,^*(), CAI Liuti², WANG Hancheng², XIONG Jing³, CHEN Xingjiang²

1. College of Agriculture, Guizhou University, Guiyang 550025,China
2. Guizhou Academy of Tobacco Science, Guiyang 550081,China
3. Bijie Tobacco Company of Guizhou Province, Bijie 551700, Guizhou, China

Received:2023-02-28 Online:2024-01-25 Published:2024-02-18

Abstract

Abstract:

To understand the differences in the structure and metabolic functions of the phyllosphere microorganisms of tobacco leaves in the control of brown spot by myclobutanil, Illumina high-throughput sequencing and Biolog-ECO technology was conducted to investigate the structure and diversity of phyllospheric microorganisms community and their metabolic function. The results showed that the phyllosphere fungi of the diseased and healthy tobacco leaves included Alternaria, Archaeorhizomyces, Boeremia, Cladosporium, etc., of which Alternaria was the dominant genus; the bacteria were Kosakonia, Pantoea and Methylobacterium. Among them, Kosakonia is the dominant genus, and the relative abundance of the Alternaria and Kosakonia was higher in diseased tobacco leaves than in healthy tobacco leaves. The relative abundance of Alternaria and Cladosporium decreased, while the relative abundance of Epicoccum and Didymella decreased and then increased when treated with 60 mL 15% myclobutanil microemulsion per 667 m² for 5, 10 and 15 d; the relative abundance of phyllosphere fungi in healthy tobacco leaves decreased in the genera Alternaria, Symmetrospora, and Didymella. The relative abundance of phyllosphere Kosakonia in diseased tobacco leaves decreased at 5 d of application and increased at 10 and 15 d, while the opposite was true for Pseudomonas. The relative abundance of Pantoea and Pseudomonas decreased at 5 and 10 d and increased at 15 d. The phyllospheric microorganism metabolic activity of brown spot-infected leaves was lower than that of healthy tobacco leaves. The metabolism of carbon sources other than α-butyric acid, L-threonine and 4-hydroxybenzoic acid by phyllosphere microorganisms was strong. The metabolic activity of phyllosphere microorganisms in diseased and healthy tobacco leaves were reduced after myclobutanil treatment, and the metabolic activity of phyllospheric microorganisms gradually recovered with the increasing of application time. The results of the study revealed the differences in phyllosphere microecological changes between diseased and healthy tobacco leaves during different periods of myclobutanil application, providing a reference for the scientific application of myclobutanil in the control of tobacco brown spot.

Key words: tobacco brown spot, myclobutanil, phyllosphere microecology, Alternaria sp., metabolic function

CLC Number:

S482.2

ZHANG Yi, WANG Feng, CAI Liuti, WANG Hancheng, XIONG Jing, CHEN Xingjiang. Phyllosphere microecology of brown spot and healthy tobacco leaves after application of myclobutanil[J]. Acta Agriculturae Zhejiangensis, 2024, 36(1): 156-167.

Figures/Tables 7

References 36

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样品采集 Sampling		施药前0 d 0 day before application	施药后5 d 5 days after application	施药后10 d 10 days after application	施药后15 d 15 days after application
健康组织Healthy tissue	样品编号Sample No.	JJJ01	JJJ11	JJJ21	JJJ31
		JJJ02	JJJ12	JJJ22	JJJ32
		JJJ03	JJJ13	JJJ23	JJJ33
	分组编号Group No.	JJJ0	JJJ1	JJJ2	JJJ3
感病组织Diseased tissue	样品编号Sample No.	JJB01	JJB11	JJB21	JJB31
		JJB02	JJB12	JJB22	JJB32
		JJB03	JJB13	JJB23	JJB33
	分组编号Group No.	JJB0	JJB1	JJB2	JJB3

样品采集 Sampling		施药前0 d 0 day before application	施药后5 d 5 days after application	施药后10 d 10 days after application	施药后15 d 15 days after application
健康组织Healthy tissue	样品编号Sample No.	JJJ01	JJJ11	JJJ21	JJJ31
		JJJ02	JJJ12	JJJ22	JJJ32
		JJJ03	JJJ13	JJJ23	JJJ33
	分组编号Group No.	JJJ0	JJJ1	JJJ2	JJJ3
感病组织Diseased tissue	样品编号Sample No.	JJB01	JJB11	JJB21	JJB31
		JJB02	JJB12	JJB22	JJB32
		JJB03	JJB13	JJB23	JJB33
	分组编号Group No.	JJB0	JJB1	JJB2	JJB3

类型 Type	样品 Sample	多样性指数Diversity index	丰富度指数Richness index	覆盖度指数 Coverage index
类型 Type	样品 Sample	Shannon	ACE	覆盖度指数 Coverage index
真菌Fungi	JJB0	0.99±0.72 d	40.08±18.27 e	0.99±0.00 a
	JJJ0	3.08±0.23 bc	111.36±30.10 cd	0.99±0.00 b
	JJB1	1.24±1.23 d	45.89±22.40 e	0.99±0.00 ab
	JJJ1	3.53±0.28 b	130.05±47.47 c	0.99±0.01 b
	JJB2	0.75±0.32 ad	62.88±40.26 de	0.99±0.00 ab
	JJJ2	4.79±0.22 a	242.72±28.89 b	0.98±0.01 c
	JJB3	2.48±0.39 c	254.21±6.89 b	0.97±0.00 c
	JJJ3	4.84±0.14 a	323.81±26.59 b	0.97±0.00 d
细菌Bacteria	JJB0	1.25±0.95 c	32.09±9.86 b	0.98±0.01 ab
	JJJ0	1.19±0.06 c	22.12±6.16 b	0.98±0.01 a
	JJB1	1.21±0.71 c	17.02±5.36 b	0.99±0.00 a
	JJJ1	0.66±0.28 c	28.47±14.15 b	0.97±0.01 ab
	JJB2	2.58±0.78 ab	264.20±299.63 ab	0.87±0.10 bc
	JJJ2	1.49±0.26 bc	328.64±192.21 ab	0.88±0.03 abc
	JJB3	3.34±1.36 a	623.03±524.08 a	0.81±0.11 c
	JJJ3	2.94±0.49 a	482.17±272.18 a	0.83±0.07 c

类型 Type	样品 Sample	多样性指数Diversity index	丰富度指数Richness index	覆盖度指数 Coverage index
类型 Type	样品 Sample	Shannon	ACE	覆盖度指数 Coverage index
真菌Fungi	JJB0	0.99±0.72 d	40.08±18.27 e	0.99±0.00 a
	JJJ0	3.08±0.23 bc	111.36±30.10 cd	0.99±0.00 b
	JJB1	1.24±1.23 d	45.89±22.40 e	0.99±0.00 ab
	JJJ1	3.53±0.28 b	130.05±47.47 c	0.99±0.01 b
	JJB2	0.75±0.32 ad	62.88±40.26 de	0.99±0.00 ab
	JJJ2	4.79±0.22 a	242.72±28.89 b	0.98±0.01 c
	JJB3	2.48±0.39 c	254.21±6.89 b	0.97±0.00 c
	JJJ3	4.84±0.14 a	323.81±26.59 b	0.97±0.00 d
细菌Bacteria	JJB0	1.25±0.95 c	32.09±9.86 b	0.98±0.01 ab
	JJJ0	1.19±0.06 c	22.12±6.16 b	0.98±0.01 a
	JJB1	1.21±0.71 c	17.02±5.36 b	0.99±0.00 a
	JJJ1	0.66±0.28 c	28.47±14.15 b	0.97±0.01 ab
	JJB2	2.58±0.78 ab	264.20±299.63 ab	0.87±0.10 bc
	JJJ2	1.49±0.26 bc	328.64±192.21 ab	0.88±0.03 abc
	JJB3	3.34±1.36 a	623.03±524.08 a	0.81±0.11 c
	JJJ3	2.94±0.49 a	482.17±272.18 a	0.83±0.07 c

环境因子及病情指数	施药前0 d	施药后5 d	施药后10 d	施药后15 d
Environmental factors and disease index	0 day before application	5 days after application	10 days after application	15 days after application
降雨量Rainfall/mm	13.60	19.00	11.30	25.40
气温Temperature/℃	18.32	18.97	17.51	16.54
空气相对湿度Relative humidity/%	82.49	83.81	78.99	90.98
病情指数Disease index	44.44	48.14	63.58	70.55

Phyllosphere microecology of brown spot and healthy tobacco leaves after application of myclobutanil

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