Expression analysis of JAZ family genes in harvested cucumber fruit under cold storage condition

doi:10.3969/j.issn.1004-1524.20231044

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (8): 1820-1831.DOI: 10.3969/j.issn.1004-1524.20231044

• Horticultural Science • Previous Articles Next Articles

Expression analysis of JAZ family genes in harvested cucumber fruit under cold storage condition

YUAN Xiao¹^,²(), JIANG Yuanyuan¹, ZHU Yunna¹, QU Shanshan³^,^*(), WANG Yukun¹, YUAN Yuan¹, WANG Bin¹^,^*()

1. Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region/College of Biology and Agriculture, Shaoguan University, Shaoguan 512005, Guangdong, China
2. College of Horticulture, South China Agricultural University/Guangdong Province Key Laboratory of Postharvest Science of Fruits and Vegetables, Guangzhou 510642, China
3. College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China

Received:2023-08-31 Online:2024-08-25 Published:2024-09-06
Contact: QU Shanshan,WANG Bin

Abstract

Abstract:

Chilling injury poses a significant challenge to the postharvest storage and transportation of cucumber fruits. Jasmonic acid (JA) plays a crucial role in regulating the chilling resistance in plants. JAZ (JA ZIM-domain) proteins act as transcription repressors of JA signaling, inhibiting both the synthesis and transmission of JA. To explore the role of JAZ family genes during cold storage of harvested cucumber fruits, JAZ family genes were identified and screened from the cucumber genome, and their sequence features, physicochemical properties, and spatial-temporal expression patterns were analyzed. The findings revealed a total of 17 JAZ genes in the cucumber genome, which could be divided into 8 subfamilies, all containing the conserved Tify or CCT domains in their protein sequences. Most of these JAZ genes responded to cold storage (5 ℃ and 10 ℃) treatments, suggesting the involvement of JAZ family proteins in regulating the chilling tolerance in cucumber fruits. However, different JAZ genes exhibited varying degrees of response to cold treatments. The promoters of cucumber JAZ family genes had numerous stress-responsive elements, though the specific types of elements varied. During 5 ℃ storage period, the expression levels of 14 JAZ genes gradually decreased, while the expression of JAZ11 significantly increased with storage time. Exogenous treatments with abscisic acid (ABA), methyl jasmonate (MeJA), melatonin (MT), as well as the silencing HHO2 and GRP3 expression in harvested cucumbers, significantly impacted the expression of JAZ family genes. These results further confirmed the involvement of cucumber JAZ family genes in regulating chilling tolerance. ABA, MeJA, and MT treatments significantly downregulated JAZ11 expression, whereas silencing HHO2 and GRP3 significantly upregulated its expression. These results together suggest that JAZ11 negatively regulates JA signaling and cold tolerance in cucumber fruits. This study provides novel insights into the physicochemical properties and expression patterns of cucumber JAZ family genes under cold conditions, and suggesting that JAZ11 may be the key factor responsible for negative regulation of chilling tolerance in harvested cucumbers among JAZ family proteins.

Key words: JAZ gene, harvested cucumber, cold storage, expression analysis

CLC Number:

S642.2

YUAN Xiao, JIANG Yuanyuan, ZHU Yunna, QU Shanshan, WANG Yukun, YUAN Yuan, WANG Bin. Expression analysis of JAZ family genes in harvested cucumber fruit under cold storage condition[J]. Acta Agriculturae Zhejiangensis, 2024, 36(8): 1820-1831.

Figures/Tables 8

References 35

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基因名称 Gene name	登录号 Accession number	氨基酸 Amino acid
基因名称 Gene name	登录号 Accession number	序列长度 Sequence length	分子量 Molecular weight/ku	等电点 Isoelectric point	不稳定系数 Instability index	脂肪指数 Aliphatic index	亲水性均值 Grand average of hydropathicity	亚细胞定位 Subcellular localization
JAZ1	CsaV3_3G030830	209	22.87	8.94	52.09	62.2	-0.63	细胞核Nucleus
JAZ2	CsaV3_2G030170	352	38.58	4.86	44.39	62.84	-0.71	细胞核Nucleus
JAZ3	CsaV3_4G002460	200	22.49	6.98	62.80	74.60	-0.55	细胞核Nucleus
JAZ4	CsaV3_5G037080	381	39.67	9.30	44.47	68.22	-0.29	细胞核Nucleus
JAZ5	CsaV3_7G033740	303	33.95	6.39	44.74	64.65	-0.87	细胞核Nucleus
JAZ6	CsaV3_7G034270	295	32.09	9.31	52.17	60.98	-0.76	细胞核Nucleus
JAZ7	CsaV3_4G007460	129	14.86	10.42	57.84	68.84	-0.43	细胞核Nucleus
JAZ8	CsaV3_1G032370	119	13.28	10.38	78.91	80.42	-0.44	细胞核Nucleus
JAZ9	CsaV3_2G030180	279	30.47	8.69	45.54	73.01	-0.51	细胞核Nucleus
JAZ10	CsaV3_6G051810	132	14.74	9.50	74.04	64.32	-0.81	细胞核Nucleus
JAZ11	CsaV3_6G007840	184	20.31	9.41	43.64	88.1	-0.32	细胞核Nucleus
JAZ12	CsaV3_1G041270	231	25.03	9.98	71.00	67.97	-0.46	细胞核Nucleus
JAZ13	CsaV3_7G006810	313	33.67	5.13	42.90	69.14	-0.62	细胞核Nucleus
JAZ14	CsaV3_2G013410	336	36.97	6.71	53.52	65.6	-0.75	细胞核Nucleus
JAZ15	CsaV3_1G007260	339	36.49	9.10	53.87	72.51	-0.24	细胞核Nucleus
JAZ16	CsaV3_2G031660	274	28.70	9.30	44.57	66.28	-0.51	细胞核Nucleus
JAZ17	CsaV3_3G046630	376	40.25	8.64	43.82	62.61	-0.62	细胞核Nucleus

基因名称 Gene name	登录号 Accession number	氨基酸 Amino acid
基因名称 Gene name	登录号 Accession number	序列长度 Sequence length	分子量 Molecular weight/ku	等电点 Isoelectric point	不稳定系数 Instability index	脂肪指数 Aliphatic index	亲水性均值 Grand average of hydropathicity	亚细胞定位 Subcellular localization
JAZ1	CsaV3_3G030830	209	22.87	8.94	52.09	62.2	-0.63	细胞核Nucleus
JAZ2	CsaV3_2G030170	352	38.58	4.86	44.39	62.84	-0.71	细胞核Nucleus
JAZ3	CsaV3_4G002460	200	22.49	6.98	62.80	74.60	-0.55	细胞核Nucleus
JAZ4	CsaV3_5G037080	381	39.67	9.30	44.47	68.22	-0.29	细胞核Nucleus
JAZ5	CsaV3_7G033740	303	33.95	6.39	44.74	64.65	-0.87	细胞核Nucleus
JAZ6	CsaV3_7G034270	295	32.09	9.31	52.17	60.98	-0.76	细胞核Nucleus
JAZ7	CsaV3_4G007460	129	14.86	10.42	57.84	68.84	-0.43	细胞核Nucleus
JAZ8	CsaV3_1G032370	119	13.28	10.38	78.91	80.42	-0.44	细胞核Nucleus
JAZ9	CsaV3_2G030180	279	30.47	8.69	45.54	73.01	-0.51	细胞核Nucleus
JAZ10	CsaV3_6G051810	132	14.74	9.50	74.04	64.32	-0.81	细胞核Nucleus
JAZ11	CsaV3_6G007840	184	20.31	9.41	43.64	88.1	-0.32	细胞核Nucleus
JAZ12	CsaV3_1G041270	231	25.03	9.98	71.00	67.97	-0.46	细胞核Nucleus
JAZ13	CsaV3_7G006810	313	33.67	5.13	42.90	69.14	-0.62	细胞核Nucleus
JAZ14	CsaV3_2G013410	336	36.97	6.71	53.52	65.6	-0.75	细胞核Nucleus
JAZ15	CsaV3_1G007260	339	36.49	9.10	53.87	72.51	-0.24	细胞核Nucleus
JAZ16	CsaV3_2G031660	274	28.70	9.30	44.57	66.28	-0.51	细胞核Nucleus
JAZ17	CsaV3_3G046630	376	40.25	8.64	43.82	62.61	-0.62	细胞核Nucleus

Expression analysis of JAZ family genes in harvested cucumber fruit under cold storage condition

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