Isolation, identification and pathogenicity of two novel goose astrovirus strains

doi:10.3969/j.issn.1004-1524.20241082

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (12): 2458-2467.DOI: 10.3969/j.issn.1004-1524.20241082

• Animal Science • Previous Articles Next Articles

Isolation, identification and pathogenicity of two novel goose astrovirus strains

DING Yingying¹^,²(), YANG Linping¹, YANG Qing¹, ZHANG Zichen¹, CAI Linying¹, YANG Kang¹, LI Chen¹, LIU Huiwen¹, BAO Guangbin¹, WANG Qing¹, WANG Guijun¹^,²^,^*()

1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
2. Anhui Provincial Key Laboratory of Veterinary Pathobiology and Disease Control, Hefei 230036, China

Received:2024-12-12 Online:2025-12-25 Published:2026-01-09

Abstract

Abstract:

To determine the etiology of disease outbreaks in goose flocks at two farms in Anhui Province of China, viruses were isolated from two suspected cases of novel goose astrovirus (GAstV) infection collected between June and July 2023 (one case involving 70-day-old growing geese), followed by whole-genome sequencing and animal regression assays. The results demonstrated the successful isolation and identification of two novel GAstV strains, which were designated as HR2306/1 and MG-23. Genomic sequence analysis revealed that both strains had a complete genome length of 7 175 nucleotides (nt). Their ORF2 sequences exhibited nucleotide and amino acid sequence homologies exceeding 97% compared with strains isolated from 2016 to 2023. The alignment of ORF2 encoding amino acid sequences indicated shared variation sites between these two strains and the strain HR2110/1 (a GAstV strain isolated by the authors’ team in 2021), suggesting that HR2306/1 and MG-23 might be evolved from HR2110/1. Animal regression assays confirmed the high pathogenicity of both strains in goslings, with clinical manifestations consistent with natural infections. Characteristic urate deposits were observed on kidney surfaces, while histopathological sections and immunohistochemical analysis revealed significant pathological changes and astrovirus antigens, identifying the kidney as a target organ of GAstV. These findings indicated that GAstV could infect 70-day-old growing geese and retain strong pathogenicity in young goslings.

Key words: novel goose astrovirus, growing goose, isolation and identification, pathogenicity

CLC Number:

S852.657

DING Yingying, YANG Linping, YANG Qing, ZHANG Zichen, CAI Linying, YANG Kang, LI Chen, LIU Huiwen, BAO Guangbin, WANG Qing, WANG Guijun. Isolation, identification and pathogenicity of two novel goose astrovirus strains[J]. Acta Agriculturae Zhejiangensis, 2025, 37(12): 2458-2467.

Figures/Tables 11

References 30

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引物名称 Primer name	引物序列(5'→3') Primer sequences (5'→3')	引物位置 Primer location
GASTV-1-F	AAACAGCGATATGGCGGC	1~18
GASTV-1-R	TGTACGACTTAGTCCAAGGAACG	823~845
GASTV-2-F	CACAGAGGCTACAATGCT	378~395
GASTV-2-R	CCTTCAACAACGACAATGG	1 665~1 683
GASTV-3-F	CAGTCCCTGTACAGATTTTA	1 446~1 465
GASTV-3-R	TCAACTTGTTCATCCTTTAC	2 792~2 811
GASTV-4-F	AGATTGATGAAGCCATTGAG	2 604~2 623
GASTV-4-R	CAGCCCGCCGTTCTGTCTGT	3 939~3 958
GASTV-5-F	AGGCTGTATCAGATATTGAT	3 755~3 774
GASTV-5-R	TCATTTTGTCATTAACGGG	5 014~5 032
GASTV-6-F	TCTGGTGAGTGGCGGACCGA	4 799~4 818
GASTV-6-R	TGTCATAACAGCCCACCAATTGTGT	6 273~6 297
GASTV-7-F	ACAACTGGACAAGGTACC	6 028~6 045
GASTV-7-R	TTTGCGGATTTTAAATGC	7 131~7 148

引物名称 Primer name	引物序列(5'→3') Primer sequences (5'→3')	引物位置 Primer location
GASTV-1-F	AAACAGCGATATGGCGGC	1~18
GASTV-1-R	TGTACGACTTAGTCCAAGGAACG	823~845
GASTV-2-F	CACAGAGGCTACAATGCT	378~395
GASTV-2-R	CCTTCAACAACGACAATGG	1 665~1 683
GASTV-3-F	CAGTCCCTGTACAGATTTTA	1 446~1 465
GASTV-3-R	TCAACTTGTTCATCCTTTAC	2 792~2 811
GASTV-4-F	AGATTGATGAAGCCATTGAG	2 604~2 623
GASTV-4-R	CAGCCCGCCGTTCTGTCTGT	3 939~3 958
GASTV-5-F	AGGCTGTATCAGATATTGAT	3 755~3 774
GASTV-5-R	TCATTTTGTCATTAACGGG	5 014~5 032
GASTV-6-F	TCTGGTGAGTGGCGGACCGA	4 799~4 818
GASTV-6-R	TGTCATAACAGCCCACCAATTGTGT	6 273~6 297
GASTV-7-F	ACAACTGGACAAGGTACC	6 028~6 045
GASTV-7-R	TTTGCGGATTTTAAATGC	7 131~7 148

年份 Year	分离毒株 Virus strains	核苷酸序列同源性 Nucleotide homology/%						氨基酸序列同源性 Amino acid homology/%		突变位点及其氨基酸 Mutation site and amino acid
		HR2306/1			MG-23			HR2306/1	MG-23	HR2306/1						MG-23
				ORF1a	ORF1b	ORF2	ORF1a	ORF1b	ORF2			26	228	380	608	614	630	228	608	614	695
												Q	T	S	T	T	A	S	T	T	T
2023	GXNN	98.6	99.3	98.3	98.4	99.2	98.7	99.0	99.4	H	S	N	T	T	T	S	T	T	A
	Q2001	97.3	99.0	97.6	97.0	98.6	97.5	97.9	98.2	H	A	N	S	A	T	A	S	A	A
	XJHT-2	98.3	99.2	99.1	97.8	98.9	99.0	99.4	99.7	H	T	N	T	T	T	T	T	T	S
2022	SD05	97.5	98.8	98.0	97.2	98.3	97.8	98.0	98.3	H	A	N	S	A	T	A	S	A	A
	GD2105	96.9	98.7	97.3	96.3	98.2	97.2	97.4	97.9	H	S	N	S	N	T	S	S	N	A
2021	HR2105/1	99.2	99.5	98.7	98.6	98.9	98.4	99.0	99.0	H	A	N	S	N	A	A	S	N	A
	HR2105/2	98.6	98.5	98.2	97.9	98.0	97.9	98.4	98.9	H	S	N	S	N	T	S	S	N	A
	HR2110/1	99.4	99.5	99.3	98.5	99.0	98.8	99.6	99.4	Q	T	N	T	T	T	T	T	T	A
	ZY02	98.6	98.7	98.0	98.0	98.2	97.7	97.9	98.3	H	S	N	S	N	T	S	S	N	A
	ZM	98.7	98.6	98.3	98.1	98.1	98.0	98.6	99.0	H	S	N	S	N	T	S	S	N	A
	GD2104	98.8	99.3	98.5	99.2	99.3	98.7	98.9	99.3	H	S	N	S	N	T	S	S	N	A
2020	HNKF-1	99.0	99.4	99.1	98.6	99.0	98.9	99.0	99.3	H	A	N	S	A	T	A	S	A	A
	HNSQ-6	98.9	99.4	99.1	98.5	98.9	98.9	99.0	99.3	H	A	N	S	A	T	A	S	A	A
2019	DY-19	99.0	99.5	98.8	98.6	99.0	98.6	98.4	98.7	H	A	N	S	A	T	A	S	A	A
	SDXT	96.3	98.2	98.2	96.0	97.8	97.9	98.0	98.3	H	A	N	S	A	T	A	S	A	A
2018	AHAU2018	97.5	98.7	98.0	97.1	98.1	97.8	97.9	98.2	H	A	N	S	A	T	A	S	A	A
	CXZ	96.7	98.5	98.3	96.3	98.0	98.0	98.2	98.4	H	A	N	S	A	T	A	S	A	A
	AHAU1	97.4	98.5	98.1	96.6	98.0	98.0	98.0	98.6	H	A	N	S	A	T	A	S	A	T
2017	SD01	97.8	98.5	97.8	97.3	98.1	97.5	97.4	97.7	H	A	N	S	A	T	A	S	A	A
	GD	97.5	98.5	97.6	96.9	98.0	97.4	98.0	98.3	H	A	N	S	A	T	A	S	A	A
	SDPY	97.1	98.6	98.2	96.7	98.2	98.1	98.4	99.0	H	A	N	S	A	T	A	S	A	T
2016	JSHA	97.2	98.6	98.0	96.9	98.0	97.7	98.0	98.3	H	A	N	S	A	T	A	S	A	A

年份 Year	分离毒株 Virus strains	核苷酸序列同源性 Nucleotide homology/%						氨基酸序列同源性 Amino acid homology/%		突变位点及其氨基酸 Mutation site and amino acid
		HR2306/1			MG-23			HR2306/1	MG-23	HR2306/1						MG-23
				ORF1a	ORF1b	ORF2	ORF1a	ORF1b	ORF2			26	228	380	608	614	630	228	608	614	695
												Q	T	S	T	T	A	S	T	T	T
2023	GXNN	98.6	99.3	98.3	98.4	99.2	98.7	99.0	99.4	H	S	N	T	T	T	S	T	T	A
	Q2001	97.3	99.0	97.6	97.0	98.6	97.5	97.9	98.2	H	A	N	S	A	T	A	S	A	A
	XJHT-2	98.3	99.2	99.1	97.8	98.9	99.0	99.4	99.7	H	T	N	T	T	T	T	T	T	S
2022	SD05	97.5	98.8	98.0	97.2	98.3	97.8	98.0	98.3	H	A	N	S	A	T	A	S	A	A
	GD2105	96.9	98.7	97.3	96.3	98.2	97.2	97.4	97.9	H	S	N	S	N	T	S	S	N	A
2021	HR2105/1	99.2	99.5	98.7	98.6	98.9	98.4	99.0	99.0	H	A	N	S	N	A	A	S	N	A
	HR2105/2	98.6	98.5	98.2	97.9	98.0	97.9	98.4	98.9	H	S	N	S	N	T	S	S	N	A
	HR2110/1	99.4	99.5	99.3	98.5	99.0	98.8	99.6	99.4	Q	T	N	T	T	T	T	T	T	A
	ZY02	98.6	98.7	98.0	98.0	98.2	97.7	97.9	98.3	H	S	N	S	N	T	S	S	N	A
	ZM	98.7	98.6	98.3	98.1	98.1	98.0	98.6	99.0	H	S	N	S	N	T	S	S	N	A
	GD2104	98.8	99.3	98.5	99.2	99.3	98.7	98.9	99.3	H	S	N	S	N	T	S	S	N	A
2020	HNKF-1	99.0	99.4	99.1	98.6	99.0	98.9	99.0	99.3	H	A	N	S	A	T	A	S	A	A
	HNSQ-6	98.9	99.4	99.1	98.5	98.9	98.9	99.0	99.3	H	A	N	S	A	T	A	S	A	A
2019	DY-19	99.0	99.5	98.8	98.6	99.0	98.6	98.4	98.7	H	A	N	S	A	T	A	S	A	A
	SDXT	96.3	98.2	98.2	96.0	97.8	97.9	98.0	98.3	H	A	N	S	A	T	A	S	A	A
2018	AHAU2018	97.5	98.7	98.0	97.1	98.1	97.8	97.9	98.2	H	A	N	S	A	T	A	S	A	A
	CXZ	96.7	98.5	98.3	96.3	98.0	98.0	98.2	98.4	H	A	N	S	A	T	A	S	A	A
	AHAU1	97.4	98.5	98.1	96.6	98.0	98.0	98.0	98.6	H	A	N	S	A	T	A	S	A	T
2017	SD01	97.8	98.5	97.8	97.3	98.1	97.5	97.4	97.7	H	A	N	S	A	T	A	S	A	A
	GD	97.5	98.5	97.6	96.9	98.0	97.4	98.0	98.3	H	A	N	S	A	T	A	S	A	A
	SDPY	97.1	98.6	98.2	96.7	98.2	98.1	98.4	99.0	H	A	N	S	A	T	A	S	A	T
2016	JSHA	97.2	98.6	98.0	96.9	98.0	97.7	98.0	98.3	H	A	N	S	A	T	A	S	A	A

Isolation, identification and pathogenicity of two novel goose astrovirus strains

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