Identification, biological characteristics and control agents screening of pathogens of curvular leaf spot disease of sorghum

doi:10.3969/j.issn.1004-1524.20240353

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (7): 1492-1500.DOI: 10.3969/j.issn.1004-1524.20240353

• Plant Protection • Previous Articles Next Articles

Identification, biological characteristics and control agents screening of pathogens of curvular leaf spot disease of sorghum

SHEN Guoqiang¹(), WANG Mingde², GUAN Yawen¹, WU Jun³^,^*(), WANG Hanrong³^,^*()

1. Shaoxing Institute of Agricultural Sciences, Shaoxing 312000, Zhejiang, China
2. Zhejiang Kaihua County Agricultural Technology Extension Center, Kaihua 324000, Zhejiang, China
3. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2024-04-12 Online:2025-07-25 Published:2025-08-20

Abstract

Abstract:

To identify the pathogen causing sorghum leaf spot, diseased sorghum leaves were collected from Pingshui Town, Shaoxing City, Zhejiang Province. Pathogen isolation, pathogenicity tests, and molecular biological identification were conducted. The biological characteristics of the pathogen were determined under different carbon sources, nitrogen sources, temperatures, pH values and light conditions. The sensitivities of the pathogen to different fungicides were tested by hyphal growth rate method. The results showed that the pathogen causing sorghum leaf spot was Curvularia lunata, and the isolated strain was designated GLYB3. Strain GLYB3 could make full use of six carbon sources, the most suitable nitrogen source was yeast powder, the optimal temperature was 25 ℃, and the optimal pH value was 7. 30% hexaconazole suspension, 43% fluopyram trifloxystrobin·suspension, 450 g·L^-1 prochloraz aqueous emulsion and 50% acetimidamine water dispersible granules had a strongly inhibitory effect on GLYB3, with EC₅₀ values of 0.001 6, 0.001 9, 0.003 1, 0.007 8 mg·L^-1, respectively. The field control experiments showed that the relative control efficiency of the four fungicides were 79.0%, 77.8%, 79.5% and 81.5%, respectively.

Key words: sorghum (Sorghum bicolor), leaf spot, biological characteristics, chemical control

CLC Number:

S435.14

SHEN Guoqiang, WANG Mingde, GUAN Yawen, WU Jun, WANG Hanrong. Identification, biological characteristics and control agents screening of pathogens of curvular leaf spot disease of sorghum[J]. Acta Agriculturae Zhejiangensis, 2025, 37(7): 1492-1500.

Figures/Tables 8

References 23

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碳源 Carbon source	菌落直径 Colony diameter	氮源 Nitrogen source	菌落直径 Colony diameter
蔗糖Source	3.57±0.06 a	酵母粉Yeast powder	3.42±0.09 a
葡萄糖Glucose	3.55±0.04 a	L-丙氨酸L-alanine	3.37±0.02 ab
可溶性淀粉Soluble starch	3.55±0.08 a	硝酸钾Potassium nitrate	3.25±0.08 b
木糖Xylose	3.55±0.16 a	牛肉粉Beef extract powder	3.18±0.12 b
肌醇Inositol	3.53±0.02 a	胰蛋白胨Tryptone	2.30±0.15 c
果糖Fructose	3.50±0.04 a	硫酸铵Ammonium sulfate	0.50±0.01 d
对照组Control	3.55±0.01 a	对照组Control	3.42±0.09 a

碳源 Carbon source	菌落直径 Colony diameter	氮源 Nitrogen source	菌落直径 Colony diameter
蔗糖Source	3.57±0.06 a	酵母粉Yeast powder	3.42±0.09 a
葡萄糖Glucose	3.55±0.04 a	L-丙氨酸L-alanine	3.37±0.02 ab
可溶性淀粉Soluble starch	3.55±0.08 a	硝酸钾Potassium nitrate	3.25±0.08 b
木糖Xylose	3.55±0.16 a	牛肉粉Beef extract powder	3.18±0.12 b
肌醇Inositol	3.53±0.02 a	胰蛋白胨Tryptone	2.30±0.15 c
果糖Fructose	3.50±0.04 a	硫酸铵Ammonium sulfate	0.50±0.01 d
对照组Control	3.55±0.01 a	对照组Control	3.42±0.09 a

药剂 Fungicide	质量浓度 Mass concentration/ (mg·L^-1)	抑制率 Inhibition rate/%	毒力回归方程 Toxicity equation	EC₅₀/(mg·L^-1)	决定系数R² Coefficient of determination
30%己唑醇悬浮剂	10^-4	44.34	y=0.194 3x+1.235 0	0.001 6	0.966 9
30% hexaconazole suspension	10^-3	63.91
	10^-2	91.30
	10^-1	100
43%氟菌·肟菌酯悬浮剂	10^-4	23.66	y=0.203 6x+1.107 1	0.001 9	0.934 2
43% fluopyram·trifloxystrobin	10^-3	49.11
suspension	10^-2	77.23
	10^-1	100
	1	100
450 g·L^-1咪鲜胺水乳剂	10^-4	12.79	y=0.237 0x+1.116 9	0.003 1	0.940 4
450 g·L^-1 prochloraz aqueous	10^-3	37.44
emulsion	10^-2	71.23
	10^-1	100
	1	100
50%啶酰菌胺水分散粒剂	10^-4	16.74	y=0.187 9x+0.927 2	0.007 8	0.981 0
50% acetimidamine water	10^-3	39.33
dispersible granules	10^-2	49.79
	10^-1	79.08
	1	90.80

药剂 Fungicide	质量浓度 Mass concentration/ (mg·L^-1)	抑制率 Inhibition rate/%	毒力回归方程 Toxicity equation	EC₅₀/(mg·L^-1)	决定系数R² Coefficient of determination
30%己唑醇悬浮剂	10^-4	44.34	y=0.194 3x+1.235 0	0.001 6	0.966 9
30% hexaconazole suspension	10^-3	63.91
	10^-2	91.30
	10^-1	100
43%氟菌·肟菌酯悬浮剂	10^-4	23.66	y=0.203 6x+1.107 1	0.001 9	0.934 2
43% fluopyram·trifloxystrobin	10^-3	49.11
suspension	10^-2	77.23
	10^-1	100
	1	100
450 g·L^-1咪鲜胺水乳剂	10^-4	12.79	y=0.237 0x+1.116 9	0.003 1	0.940 4
450 g·L^-1 prochloraz aqueous	10^-3	37.44
emulsion	10^-2	71.23
	10^-1	100
	1	100
50%啶酰菌胺水分散粒剂	10^-4	16.74	y=0.187 9x+0.927 2	0.007 8	0.981 0
50% acetimidamine water	10^-3	39.33
dispersible granules	10^-2	49.79
	10^-1	79.08
	1	90.80

处理 Treatment	施药前平均病情指数 Average disease index before application	施药2次后10 d 10 days after 2 times of application
处理 Treatment	施药前平均病情指数 Average disease index before application	平均病情指数 Average disease index	相对防治效果平均值 Average value of relative control efficiency/%
30%己唑醇悬浮剂1 000倍液 30% hexaconazole suspension 1 000 times	1.39	1.84	79.0
43%氟菌·肟菌酯悬浮剂1 500倍液 43%fluopyram·trifloxystrobin suspension 1 500 times	1.32	1.85	77.8
450 g·L^-1咪鲜胺水乳剂1 000倍液 450 g·L^-1 prochloraz aqueous emulsion 1 000 times	1.44	1.85	79.5
50%啶酰菌胺水分散粒剂1 000倍液 50% acetimidamine water dispersible granules 1 000 times	1.50	1.79	81.5
对照组Control group	1.47	9.23	/

Identification, biological characteristics and control agents screening of pathogens of curvular leaf spot disease of sorghum

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