Abstract:

This paper aims to find out a suitable method for extracting, purifying and analyzing endogenous gibberellin A3 of rice panicle by highperformance liquid chromatography method. To deal with the existence of large amount of pigments, polyphenols and other impurities,pigment was eliminated by chloroform and then gibberellin A3 was purified by liquidliquid extraction of three steps. On the basis of former reports, the highperformance liquid chromatographic conditions for analyzing endogenous hormone of plant were improved. It was found that the optimal chromatographic conditions included that detection wave length was made by the UV detector at 254 nm and the mobile phase was composed of acetonitrilewateracetic acid liquid (volume ratio 20∶79∶1) at the velocity of 1.0 mL·min-1, et al. The peak shape was good and retention time was stable under these chromatographic conditions. The results showed that the add mark recovery rate of gibberellin A3 was above 80% and the relative standard deviation was less than 3% according to this test procedure, which demonstrated that the test procedure could detect endogenous gibberellin A3 of rice panicle excisely.

Key words: rice, panicle, highperformance liquid chromatography, gibberellin A3