Use of green fluorescent protein gene to label Bacillus subtilis strains

›› 2009, Vol. 21 ›› Issue (3): 0-206.

• 论文 •

Use of green fluorescent protein gene to label Bacillus subtilis strains

SHEN Wei—feng;NIU Bao-long;WENG Hong-biao;LIU Yah;HE Li—hua;MENG Zhi—qi*

Institute of sericulatre，Zhejiang Academy of Agricultural Sciences，Hangzhou 310021，China

Received:1900-01-01 Revised:1900-01-01 Online:2009-05-25 Published:2009-05-25

Abstract

Abstract: The xylR promoter from B．subtilis 168 chromosomal DNA was ligated with GFP germ from plasmid GFPuv．The expression fragment xylR-GFP was inserted into the shuttle-vector pIM．The recombinant vector plM-GFP was successfully
obtained．The pIM-GFP was transformed into BS523 strain．Transformants were identified by green fluorescence observation with microscope．The results showed that pIM-GFP was successfiflly integrated into the strain BS523．

Key words: Bacillus subtilis, green fluorescent protein, recombinant vector

SHEN Wei—feng;NIU Bao-long;WENG Hong-biao;LIU Yah;HE Li—hua;MENG Zhi—qi*. Use of green fluorescent protein gene to label Bacillus subtilis strains[J]. , 2009, 21(3): 0-206.

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Use of green fluorescent protein gene to label Bacillus subtilis strains

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