Use of green fluorescent protein gene to label Bacillus subtilis strains

›› 2009, Vol. 21 ›› Issue (3): 0-206.

• 论文 •

Use of green fluorescent protein gene to label Bacillus subtilis strains

SHEN Wei—feng;NIU Bao-long;WENG Hong-biao;LIU Yah;HE Li—hua;MENG Zhi—qi*

Institute of sericulatre，Zhejiang Academy of Agricultural Sciences，Hangzhou 310021，China

Received:1900-01-01 Revised:1900-01-01 Online:2009-05-25 Published:2009-05-25

Abstract

Abstract: The xylR promoter from B．subtilis 168 chromosomal DNA was ligated with GFP germ from plasmid GFPuv．The expression fragment xylR-GFP was inserted into the shuttle-vector pIM．The recombinant vector plM-GFP was successfully
obtained．The pIM-GFP was transformed into BS523 strain．Transformants were identified by green fluorescence observation with microscope．The results showed that pIM-GFP was successfiflly integrated into the strain BS523．

Key words: Bacillus subtilis, green fluorescent protein, recombinant vector

SHEN Wei—feng;NIU Bao-long;WENG Hong-biao;LIU Yah;HE Li—hua;MENG Zhi—qi*. Use of green fluorescent protein gene to label Bacillus subtilis strains[J]. , 2009, 21(3): 0-206.

[1]	HE Yu, LYU Weiguang, LI Shuangxi, ZHENG Xianqing, ZHANG Hanlin, ZHANG Juanqin, ZHANG Haiyun, BAI Naling, LIU Shanliang. Effects of γ-polyglutamic acid fermentation broth on growth of pakchoi and utilization rate of nitrogen and phosphorus fertilizer [J]. Acta Agriculturae Zhejiangensis, 2023, 35(2): 329-337.
[2]	GAO Jing, FANG Wei, GU Jiayue, YAN Shuxian, SHAO Shuai, LIANG Chenfei, QIN Hua, CHEN Junhui, XU Qiufang. Colonization of fluorescently labeled Bacillus amylolyticus WK1 in Carya cathayensis Sarg. and soil [J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 77-86.
[3]	GUI Xueer, WANG Zhi, LI Siting, HE Mengchu, ZHU Jie, FENG Shibin, WU Jinjie. Effect of chicken-derived compound probiotics on immunoglobulin and toll-like receptor pathway of white feather broilers [J]. , 2020, 32(9): 1609-1614.
[4]	XU Li, CHEN Xiaojie, CAO Jingting, LIU Chuchu, DING Ting, JIANG Teng. Resistance mechanism of biocontrol strain DZSG23 against wheat scab [J]. , 2020, 32(11): 2001-2008.
[5]	ZHOU Yanchao, WU Yanhong, TIAN Xingwu, ZHOU Haixia, HAN Zeyu, LIU Jiqing, LAN Zhiqian, ZHANG Xueyan. Effect of nano-carbon sol and Bacillus subtilis on cucumber growth and soil environment [J]. , 2019, 31(3): 392-400.
[6]	SUO Guifang, LYU Haohao, WANG Yuying, LIU Yuxue, HE Lili, YANG Shengmao. Preparation technology and properties analysis of biochar-based microbial fertilizers [J]. , 2018, 30(7): 1218-1228.
[7]	ZOU Gaoxi, ZHAO Chuntian, QIU Juanping. Optimization of fermentation technology of biocontrol bacterium Bacillus subtilis 210 by response surface analysis [J]. , 2017, 29(5): 799-805.
[8]	HUANG Huayi, WANG Jialin, MA Rong, LIANG Yingmei, TIAN Chengming. Antifungal activities of Bacillus subtilis STO-12 and analysis on its antifungal substances [J]. , 2017, 29(1): 81-88.
[9]	LI Jing 1，2，TONG Jin 1，2，LUO Mingyin 1，LI Chenwei 1， XIAN Jie 1. Construction of the mutant library of Bacillus subtilis lipaseA gene and study on its transesterification efficiency of biodiesel [J]. , 2016, 28(5): 864-.
[10]	CHEN Hong-na, LI Jian-wen, JIANG Ao, SUN Wen-xiu. Prokaryotic expression and activity analysis of thioredoxin reductase from Bacillus subtilis [J]. , 2016, 28(12): 2054-2059.

Use of green fluorescent protein gene to label Bacillus subtilis strains

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 10

Recommended Articles

Metrics

Comments