Abstract: Tissue culture was carried out by using peach stems of Zhenxuan NO.1， Dongxixiaoxiantao and Maotao as the external plants. Effects of different sterilization methods， base media， kinds and concentration of phytohormone on the primary culture， multiplication culture and rooting culture of shoot tips of three peach rootstocks were studied. The results showed that the most optimal sterilization method for primary culture was sterilization with 75% alcohol for 30 s or 0.1% HgCl2 for 3 min， in which condition the higher survival rate of the bottom part of explants was found. The MS medium added with 0.5 mg·L-1 6\|BA and 0.2 mg·L-1 IBA was most optimal for the primary culture of Dongxixiaoxiantao and Zhenxuan NO.1， whose survival rate was 73.33% and 76.67%， respectively. The MS medium added with 1.5 mg·L-1 6\|BA and 0.2 mg·L-1 IBA was suitable for the multiplication culture of Maotao and Zhenxuan NO.1. The MS medium added with 2.0 mg·L-1 6\|BA and 0.5 mg·L-1 IBA was suitable for multiplication culture of Dongxixiaoxiantao. The 1/2 MS medium was the most suitable medium for rooting culture. The 1/2 MS medium added with 1.0 mg·L-1 IBA was optimal for rooting culture of Maotao and Zhenxuan NO.1， and 1/2 MS medium added with 0.5 mg·L-1IBA was suitable for Dongxixiaoxiantao.

Key words: peach, rootstock, shoot tip culture, primary culture