Abstract: Twenty\|four Vitis germplasm were used as materials for analyzing their genome polymorphism by ISSR markers. Six primers with stable amplification and rich polymorphism selected from 50 primers and used for ISSR amplification. A total of 59 bands were generated， of which 53 bands were polymorphic bands （90.0%）. Based on ISSR amplification and analysis by NTSYSpc2.10e software， the genetic similarity coefficient varied from 0.42 to 0.88 with an average of 0.6495. The clustering dendrogram was constructed by UPGMA method. Twenty-four Vitis materials were divided into two major groups at the similarity of 055. The first group included 10 cultivars of Euro-American hybrids， 7 cultivars of V. vinifera and 1 cultivar of China European hybrid（V. pseudoreticulat ×V. vinifera）. The second group included 3 cultivars of American hybrids， 1 cultivar of Riverside grape， 1 cultivar of V. berlandieri grape and 1 cultivar of East Asia grape. The results showed that American hybrids and Euro-American hybrids had a distant relationship， while Euro-American hybrids and V. vinifera had a closer similarity. In addition， only one specific band was amplified from Huajia No.8 using primer BC820 and Kangzhen No.3 using primer BC847， respectively, which would be helpful to provide reference for identification of grape varieties or lines using ISSR markers.

Key words: Vitis, ISSR, genetic relationship, cluster analysis