Abstract: A transient cold condition can induce the fruiting body formation of P. pulmonarius. A subtractive cDNA library using suppressive subtractive hybridisation (SSH) was constructed for a better understanding of the molecular basis of fruitingbody formation of P. pulmonarius after cold stimulation. Based on the SSH library，a total of 185 highquality ESTs were identified by upregulated expression. BLAST searching against the Basidiomycota genomes from JGI showed that 136 EST sequences were homologous to previously known genes，and there were 49 sequences with unknown function. A total of 139 ESTs were categorized into 23 functional groups. This information can help us better understand the molecular basis underlying the fruiting induced by cold stimulation in P. pulmonarius．

Key words: Pleurotus pulmonarius, fruiting after cold stimulation, suppressive subtractive hybridization, cDNA library