Abstract: In this study， the infection effect of canker pathogens was determined by the inoculation of the branches of Hongyang kiwifruit in vitro under different pathogenicity，inoculation concentration，cultivation humidity，and inoculation methods.Then，the resistance of 24 kiwifruit varieties were evaluated.The result showed as follows: pathogens with high pathogenicity，high concentration，high humidity，and wound area of branch all had remarkable effect on the disease index of kiwifruit branches；Different kiwifruit varieties showed different resistance to canker.Among the branches of 24 varieties，White and Xuxiang showed high resistance; Miniwhite，Jinqui，Excellentgreenflesh(GHZ201201)and Maoxiong showed resistance；Dahong，Zaoxian，Zaoyan and Excellentyellowflesh(YHZ201201)showed highly susceptible characteristic.In addition，the potted seedlings of White，Bruno and Hongyang were inoculated with canker pathogens and the related physiological parameters between inoculated plants and control plants were measured. The result showed that the resistance rank of 3 varieties with potted seedlings was correspond to that with blanches in vitro.Enzymatic activity of kiwifruit varieties with different resistance had a linear relationship with their disease index.CAT enzyme activity in phloem of healthy plants had the highest relevancy(r=0999*)，and CAT enzyme activity of blades took the second place(r=0994*).Relevancies between disease index and POD enzyme activity of healthy plants phloem and blades were r=0979 and r=0969 respectively.The relevancy of SOD enzyme activity were r=-0924 and r=-0938.Soluble sugar，soluble starch and soluble protein also had a linear relation with disease index.Soluble protein had the highest relevancy with disease index of r=-0972 and r=-0962 in healthy plants phloem and blades.The relevancy between soluble sugar and disease index was r=-0934 and r=-0940.To sum up，the resistance of different varieties of kiwifruit to canker had a significant difference，and the activity of CAT，POD and SOD， as well as the content of soluble protein and soluble sugar could be used as the physiological index in the identification of kiwifruit resistance to canker．

Key words: kiwifruit, P. syringae pv. actinidiae, disease resistance