Abstract: Proteins of WRKY family，one of the largest families of transcription factors，play an important role in regulation of plant growth and development.In this study，The full length cDNA sequence of OsWRKY71 gene was cloned by SMART\|RACE\|PCR from rice（Oryza sativa L.），which was named as OsWRKY71（GenBank Accession No.KJ137000）. The full length cDNA of OsWRKY71 is 1 245 bp，which includes 1 047 bp complete CDS and encodes 348 amino acid residues with a putative molecular mass of 3828 kDa. The bioinformatics characterization indicated that the protein encoded by OsWRKY71 gene has a typically conserved domain structure of WRKY family，and it shared 69% amino acid sequences similarity with WRKYs of wheat. It shared over 68% amino acid sequences similarity with WRKYs of barley. The results of quantitative real\|time PCR（qRT\|PCR） showed that the highest expression level appeared in flag leaf at heading stage. The lowest expression level appeared in root. The expression of OsWRKY71 was up\|regulated at first and then down\|regulated when the resistant line IR28 was inoculated with Ustilaginoidea virens，and expression was suppressed in the infectious line Yongyou 9. These results implied that OsWRKY71 was a pathogen\|responsive gene and might play an important role in rice pathogen resistance.

Key words: rice, WRKY transcription factor, expression analysis, quantitative real\, time PCR