Abstract: The coding sequence （CDS）， nucleotide diversity and amino acid sequence of Atrn gene from Homo sapiens， Bos Taurus， Bos mutus， Balaenoptera acutorostrata， Bubalus bubalis， Canis lupus， Callithrix jacchus， Chinchilla lanigera， Ceratotherium simum， Condylura cristata， Dasypus novemcinctus， Echinops telfairi， Elephantulus edwardii， Felis catus， Heterocephalus glaber， Macaca fascicularis， Macaca mulatta， Mustela putorius furo， Mus musculus， Nomascus leucogenys， Pteropus alecto， Rattus norvegicus， Sus scrofa and Spermophilus tridecemlineatus were analyzed using the method of bioinformatics. The results showed that the Atrn gene CDS had abundant genetic diversity between intraspecific and interspecific species， and a total of 899 polymorphic sites were detected from 61 sequences of 24 species which generated 31 haplotypes. The results also showed that Atrn has trans\|membrane domain and signal peptide in N\|terminal and belonged to membrane protein or secretion protein which were located on the biofilm. The protein was hydrophilic， instable and alkalescent， with an isoelectric point of 7.17. The main structural element of predicted secondary structure of Atrn were random coil and alpha helix.

Key words: species, Atrn gene, bioinformatics analysis