Cloning and analysis of rDNA ITS sequences from Vanda plants#br#

Cloning and analysis of rDNA ITS sequences from Vanda plants#br#

（College of Life Science， Taizhou University， Jiaojiang 318000， China）

Online:2015-08-25 Published:2015-08-21

Abstract

Abstract: To provide evidences for molecular identification and genetic diversity studies， rDNA ITS sequences of 7 Vanda plants were isolated. ITS sequences were cloned by polymerase chain reaction using leaf genomic DNA as templates， and bioinformatic analysis was performed. Results indicated that the full length of ITS sequences ranged from 653 to 657 bp， and the sequences of 58S and ITS2 were conserved with an identical length of 163 and 260 bp， respectively; however， sequence length of ITS1 varied from 230-234 bp. The 58S sequences of 7 Vanda plants were identical， while some cases of transitions， transversion， and deletions/insertions were identified in both ITS1 and ITS2 sequences. Phylogenetic analysis results showed that the average genetic distance of 7 Vanda plants was 0021， and they were divided into 3 groups.

Key words: Vanda, ITS sequence, cloning, molecular identification

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Cloning and analysis of rDNA ITS sequences from Vanda plants#br#

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