PCR identification of the wheat polymers with high quality subunit and powdery mildew resistance Pm4a gene

（1. Key Laboratory of Crop Breeding South Zhejiang， Wenzhou 325006， China; 2. Institute of Crop Research, Wenzhou Academy of Agricultural Sciences， Wenzhou 325006， China）

Online:2016-04-25 Published:2016-04-27

Abstract

Abstract: In order to improve the quality， yield and disease resistance of wheat lines （varieties）， Ningchun 4， and Yangmai 19 which have 1Dx5+1Dy10 subunit and Pm4a powdery mildew resistance gene were used as parents in present study， respectively. Genomic DNA was extracted from the 1/31/2 grain endosperm of their F2 individuals. PCR identification was conducted with the specific markers of 1Dx5 subunit and Pm4a genes. The results showed that 51 seeds were identified as 1Dx5 subunit and Pm4a gene polymers among 100 F2 individuals. PCR identification used in present study could overcome the time consuming and being easy to confuse the subunits with similar migration rate of SDSPAGE electrophoresis， get rid of the restrictions of climate and farming season of field and greenhouse identification， and improve the efficiency and accuracy of identification greatly. With further evaluation of the selected polymers， the lines with good agronomic traits， high yield and quality， resistance to powdery mildew would be hopefully developed.

Key words: wheat, high molecular weight glutenin subunit （HMWGS）, powdery mildew, polymer, PCR

LIU Yongan1，2，PAN Binrong1，2，YUE Gaohong1，2，MEI Xixue2，XU Likui1，2，*，ZHANG Zongchen1，2，ZHOU Zhihui1，2. PCR identification of the wheat polymers with high quality subunit and powdery mildew resistance Pm4a gene[J]. .

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PCR identification of the wheat polymers with high quality subunit and powdery mildew resistance Pm4a gene

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