Cloning and expression analysis of BoBURP1 gene from Brassica oleracea var. italica

doi:10.3969/j.issn.1004-1524.2016.09.07

›› 2016, Vol. 28 ›› Issue (9): 1501-1507.DOI: 10.3969/j.issn.1004-1524.2016.09.07

• Horticultural • Previous Articles Next Articles

Cloning and expression analysis of BoBURP1 gene from Brassica oleracea var. italica

ZHANG Yan-ru, YU Ke-ke, GONG Xiu, ZHOU Xiu-qian, ZHU Qi, XU Xin, ZHU Xin, LYU Yi, JIANG Xiao-ying, JIANG Ming^*

College of Life Sciences, Taizhou University, Jiaojiang 318000, China

Received:2016-05-04 Online:2016-09-15 Published:2016-11-23

Abstract

Abstract: BURPs are plant-specific proteins, which play important roles in growth, development as well as stress responses. In this study, a BURP gene, designated as BoBURP1, was isolated from broccoli. Bioinformatics analysis has been performed, and expression patterns in different organs were then conducted by means of RT-PCR. Results indicated that the genomic DNA of BoBURP1 was 2 030 bp in length with 2 introns, and the full length coding sequence was 1 872 bp in size encoding 623 amino acids. The molecular weight of the deduced protein was 70.7956 ku with an isoelectric point of 8.65. Phylogenetic analysis results revealed that BoBURP1 and its 12 homologous sequences could be divided into three groups, and the highest identities existed among Cruciferae plants which clustered in the same group. RT-PCR results showed BoBURP1 gene expressed in roots, stems, leaves, flower buds, flowers and young siliques, and the highest was observed in leaves, flower buds and flowers, while the lowest were found in both roots and stems.

Key words: Brassica oleracea var. italica, BURP, cloning, expression

CLC Number:

S635.3
Q78

ZHANG Yan-ru, YU Ke-ke, GONG Xiu, ZHOU Xiu-qian, ZHU Qi, XU Xin, ZHU Xin, LYU Yi, JIANG Xiao-ying, JIANG Ming. Cloning and expression analysis of BoBURP1 gene from Brassica oleracea var. italica[J]. , 2016, 28(9): 1501-1507.

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Cloning and expression analysis of BoBURP1 gene from Brassica oleracea var. italica

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